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简介：Tumor-targetingantibodieswereinitiallydefinedasagroupoftherapeuticmonoclonalantibodies(mAb)thatrecognizetumor-specificmembraneproteins,blockcellsignaling,andinducetumor-killingthroughFc-driveninnateimmuneresponses.However,inthepastdecade,ampleevidencehasshownthattumor-targetingmAb(TTmAb)eradicatestumorcellsviaactivationofcytotoxicTcells(CTLs).Inthisreview,wespecificallyfocusonhowTTmAbsinduceadaptiveanti-tumorimmunityanditspotentialincombinationtherapywithimmunecytokines,checkpointblockade,radiation,andenzymetargetedsmallmoleculedrugs.ExploringthemechanismsofthesepreclinicalstudiesandretrospectiveclinicaldatawillsignificantlybenefitthedevelopmentofhighlyefficientandspecificTTmAb-orientedanti-tumorremedies.

简介：Overthepastdecades,cellsurfacecharge,althoughexperimentallyobserved,hasnotbeenwellunderstoodparticularlyfromtheviewpointofbiophysics.Ourrecentstudieshaveshownthatallcancercellsexhib让negativesurfacechargesthataredirectlyproportionaltothesecretedlacticacid,auniquecancermetaboliccharacteristic:highrateofglycolysis.Wehavethereforedesignedanddevelopedasetofelectrically-charged,fluorescent,andsuper-paramagneticnanoprobes,capableofsensitivedetectionofcancercellsbasedonthesurfacecharges.Theseprobesareutilizedtobindontocellsviaelectrostaticreactionforcaptureandmagneticseparation.Inthisfashion,weareabletocharacterizecellsurfacechargesthatareregulatedbydifferentmetabolicpatterns,thereforeeffectivelydistinguishingthecancercellsfromthenormalcells.All22cancercellsofdifferentorgansarefoundtobenegativelychargedthereforeboundstronglybythepositively-chargednanoprobes,whereasthenormalcellsshowinsignificantbindingtothenanoprobesofeitherchargesigns(positiveornegative).Thisfindingsuggeststhatalltestedcancercellsarenegatively-chargedandnormalcellsareeithercharge-neutralorslightlypositive.Fordiagnosis,cancercellscanbedetected,electrostaticallybound,andmagneticallyseparatedinbloodbychargedandsuper-paramagneticnanoprobes.Intherapeutics,circulatingcancercells(CTCs)canbefilteredandremovedinacontinuousfashiontoreducetheriskofcancermetastasis.Ifsuccessful,thisnewnanotechnologywillrevolutionizeearlycancerdiagnosisandpotentiallyenablenewtherapeuticsinclinicalsettings.

简介：Insulinsecretorygranules(ISGs),agroupofdistinguishingorganellesinpancreaticβcells,areresponsibleforthestorageandsecretionofinsulintomaintainbloodglucosehomeostasis.ThemolecularmechanismsofISGbiogenesis,maturation,transportation,andexocytosisarestilllargelyunknownbecausetheproteinsinvolvedinthesedistinctstepshavenotbeenfullyidentified.Subcellularfractionationbydensitygradientcentrifugationhasbeensuccessfullyemployedtoanalyzetheproteomesofnumerousorganelles.However,useofthismethodtoelucidatetheISGproteomeislimitedbyco-fractionatedcontaminantsbecause1SGsareverydynamicandhaveabundantexchangesorcontactswithotherorganelles,suchastheGolgiapparatus,lysosomes,andendosomes.Inthisstudy,wedevelopedanewstrategyforidentifyingISGproteinsbyproteincorrelationprofiling(PCP)-basedproteomics,whichincludedISGpurificationbyOptiPrepdensitygradientcentrifugation,label-freequantitativeproteome,andidentificationofISGproteinsbycorrelatingfractionationprofilesbetweencandidatesandknownISGmarkers.Usingthisapproach,wewereabletoidentify81ISGproteins.Amongthem,TM9SF3,anine-transmembraneprotein,wasconsideredahighconfidenceISGcandidateproteinhighlightedinthePCPnetwork.FurtherbiochemicalandimmunofluorescenceassaysindicatedthatTMgSF3localizedinISGs,suggestingthatitisapotentialnewISGmarker.

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简介：Theadaptivetreatmenttolerance(ATT)ofcancercellsisthemainencumbrancetocancerchemotherapy.Apotentialsolutiontothisproblemistotreatcancercellswithmultipledrugsusingnanoparticles(NPs).Inthisstudy,wetestedtheco-administrationofcurcumin(Cur)anddoxorubicin(Dox)toMCF-7resistantbreastcancercellstoblocktheATTandelicitefficientcellkilling.Drugswereco-administeredtocellsbothsequentiallyandsimultaneously.Sequentialdrugco-administrationwascarriedoutbypre-treatingthecellswithalbuminnanoparticles(ANPs)loadedw让hCur(Cur@ANPs)followedbytreatmentwithDox-loadedANPs(Dox@ANPs).Simultaneousdrugco-administrationwascarriedoutbytreatingthecellswithANPsloadedwithboththedrugs(Cur/Dox@ANPs).Wefoundthatthesimultaneousdrugco-administrationledtoagreaterintra-cellularaccumulationofDoxandcellkillingwithrespecttothesequentialdrugco-administration.However;thesimultaneousdrugco-administrationledtoalowerintracellularaccumulationofCurwithrespecttothesequentialdrugco-administration.WeshowedthatthisresultwasduetotheaggregationandentrapmentofCurinthelysosomesassoonasitwasreleasedfromCur@ANPs,aphenomenoncalledlysosomotropism.Incontrast,thesimultaneousreleaseofDoxandCurfromCur/Dox@ANPsintothelysosomesledtolysosomalpHelevation,which,inturn,avoidedCuraggregation,ledtolysosomeswellinganddrugreleaseinthecytosol,andfinallyprovokedefficientcellkilling.Ourstudyshedthelightonthemolecularprocessesdrivingthetherapeuticeffectsofanti-cancerdrugsco-administeredtocancercellsindifferentmanners.

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