简介:Greyself-organizingmap(GSOM)modelisproposedandappliedinthedetectionofintrusion.ThroughtheimprovementoftheweightadjustmentusingtheGRC(greyrelationalcoefficient),thetrainingresultsofSOMgetbetter.Inthedetectionofdenyofservice(DOS)attacks,thismodelcanconsidertherelativityofthedatasetofDOSattacks.Finally,theexperimentsontheDOSdatasetconfirmtheirvaliditiesandfeasibilitiesoverthisGSOMmodel.
简介:Fluorescenceliftimeimaging(FLIM)ofmodifiedhydrophobicbodipydyesthatactasfluorescentmolecularrotorsshowsthatthefluorescencelifetimeoftheseprobesisafunctionofthemicroviscosityoftheirenvironment.Incubatingcellswiththesedyes,wefindapunctateandcontinuousdistributionofthedyeincells.Theviscosityvalueobtainedinwhatappearstobeendocytoticvesiclesinlivingcellsisaround100timeshigherthanthatofwaterandofcellularcytoplasm.Time-resolvedfluorescenceanisotropymeasurementsalsoyieldrotationalcorrelationtimesconsistentwithlargemicroviscosityvalues.Inthisway,wesuccessfullydevelopapracticalandversatileapproachtomapthemicroviscosityincellsbasedonimagingfluorescentmolecularrotors.
简介:排水量操作员是一重要、静止在地图归纳的一个热话题。在归纳产品,符号一定不含糊、容易察觉、容易地听说,它在特征之中做空格竞争在地图的进程的一个重要障碍反对排水量。通过繁殖过程,在目标之间的空间冲突可以被散布,更多的目标可以漂流进它。为了维持符号,在符号的对象,一些不重要的符号或部分之间的平衡和空间关系应该根据视觉图形的分辨率阀值在限制下面被弄歪在地图特征之中弄明白空格竞争。包括位置,易读性和特征的三限制为符号平衡和空间关系的维护是重要的,它在这篇论文被讨论。骨骼被介绍表示区域目标考虑特征,一个区域目标能在被分开到在构造根据他们的重要性分开教主的联盟者。然后,有限元素方法被用于地图目标的排水量和失真,为有限元素方法的参数的策略在被讨论。
简介:SLAMisoneofthemostimportantcomponentsinrobotnavigation.ASLAMalgorithmbasedonimagesequencescapturedbyasingledigitalcameraisproposedinthispaper.Bythisalgorithm,SIFTfeaturepointsareselectedandmatchedbetweenimagepairssequentially.Afterthreeimageshavebeencaptured,theenvironment’s3Dmapandthecamera’spositionsareinitializedbasedonmatchedfeaturepointsandintrinsicparametersofthecamera.Arobustmethodisappliedtoestimatethepositionandorientationofthecameraintheforthcomingimages.Finally,arobustadaptivebundleadjustmentalgorithmisadoptedtooptimizetheenvironment’s3Dmapandthecamera’spositionssimultaneously.Resultsofquantitativeandqualitativeexperimentsshowthatouralgorithmcanreconstructtheenvironmentandlocalizethecameraaccuratelyandefficiently.
简介:Thereareabout17chromosomesinyeastSaccharomycescerevisiae.Amiddlesizedchromosome,chromosomeV,waschoseninthisworkforstudyingandconstructingthephysi-calmaps.ChromosomeVfromstrainA364awasisolatedbypulsed-fieldgradientgelelectrophoresis(PFGE).GelslicescontainingchromosomeVDNAweredigestedwithtworarecuttingenzymes,NotⅠandSfiⅠ,andthree6-Ntrecognizingenzymes,SmaⅠ,SstⅡandApaⅠ.Severalstrategies-partialorcompletedigestions,digestionwithdifferentsetsoftwoenzymes,andhybrid-izationwithclonedgeneticallymappedprobes(CAN1,URA3,CEN5,PRO3,CHO1,SUP19,RAD51,RAD3)——wereusedtoaligntherestrictionfragments.Thereare9,9,15,17,and20sitesforNotⅠ,SfiⅠ,SmaⅠ,SstⅡandApaⅠrespectivelyinthemapoftheA364achromosomeV.Itstotallengthwascalculatedtobe620Kb(Kilo-bases).Thedistributionsofthecuttingsitesforthesefiveenzymesthroughthewholechromosomearenotuniform.Acomp-arisonbetweenthephysicalmapandthegeneticmapwasalsomade.
简介:Limitationsofdifferencemapsshowingcirculationanomaliesareanalyzed,andthedefinitionofthelocalpatternanaloguecoefficient(LPAC)isgiventogetherwiththeprocedureforconstructingsuchamap,followedbyanexampleillustratingitsusefulapplicationincirculationanomaly.
简介:Basedonthedataofearthquakecentroidmomenttensor(CMT)solution,P-wavefirstmotionfocalmechanismsolutionanddeepholebreakouts,apresent-daytectonicstressmapforeasternAsiaregioniscompiled.Theorigi-nalstressdataaresmoothedforevery200km′200kmareabytakingtheaverageofallstressindicatorswithineachsub-region.Thestressmapshowsthespatialdistributionoftheorientationofprincipalstressaxesandthestressregimes.AnearthquakefocalmechanismmapfortheeasternAsiaisalsogiven.Themapsoforientationofprincipalstressaxesshowthat,apartfromthestronginfluenceofthecollisionbetweentheIndianOceanplateandEurasianplate,thepresent-daytectonicstressineasternAsiaissignificantlyaffectedbytheback-arcextensionofthesubductionzones.ThejointeffectofthecontinentalcollisionattheHimalayaarcandback-arcextensionintheBurmaarcregionmayberesponsiblefortheremarkablerotationoftheprincipalstressorientationsinsoutheasternpartoftheTibetplateau.ThejointactionofthecollisionbetweenthePhilippineSeaplateandEurasianplateatTaiwanIslandandtheback-arcextensionoftheRyukyuarcaffectthestressfieldineasternpartofChina.Therearenostrongearthquakesinthepresentdayinthevastback-arcregionoftheJavatrenchsubductionzone.Theback-arcextensiontheremaycreateaconditionfavorabletothesouthwardflowofthelithospherematerialinsoutheasternAsia.IntheinnerpartoftheTibetplateauregion,roughlydemarcatedbytheKunlunmountain,thenorthernandnortheasternpartisabroadintracontinentalcompressivezone,whilethesouthernandsouthwesternpartisgenerallyinanormal-faultingstressstate.
简介:摘要目的探讨一例由MAP2K1基因变异引起的心-面-皮肤综合征(cardio-facio-cutaneous syndrome, CFCS)患儿基因型与表型的对应关系。方法收集疑似CFCS患儿及其父母的外周血样,提取基因组DNA,对患儿及父母采用全外显子组测序,最后用Sanger测序对可疑变异位点进行验证。结果患儿为男性,具有典型的CFCS面容以及发育迟滞。经全外显子组检测分析发现患儿的MAP2K1基因第3外显子上存在1个新发的杂合错义变异c.389A>G(p.Tyr130Cys),Sanger测序验证了该结果,根据最新美国医学遗传学与基因组学学会评级指南判定为疑似致病性变异。结论患儿临床未发现心脏结构、功能的异常以及癫痫发作,但具有典型面容及皮肤异常,伴有生长发育迟缓、智能障碍、泌尿系统结构异常。该病例丰富了MAP2K1基因变异导致的CFCS表型谱。
简介:High-densitymarkersarenecessaryformap-basedcloningofricegenes,butthecurrentlyavailablemarkersarenotsatisfactoryenough.InDel(insertion-deletionlengthpolymorphism)andSNP(singlenucleotidepolymorphism)arethenewgenerationofmolecularmarkersandcanbasicallymeettheneedoffinemapping.InDelandSNPmarkerscanbedevelopedthroughbioinformatics.Thesemarkersarevaluablemarkerswiththecharactersoflowcost,highspecificityandstability.ThisarticleintroducedthemethodsfordesigningInDelandSNPmarkers,takingthemappingofaricerolledleafgeneasanexample.Inaddition,somekeyfactorsinimprovingthedesignefficiencywerealsodiscussed.
简介:Thegenerationofhigh-resolutionDEMfrominterferometricSARhasresultedintheneedforaccurateandefficientmethodsof2-dimensionalphaseunwrapping.Inthispaper,wegiveabriefdescriptionofthemathematicalbaseofphaseunwrapping,andadetaileddescriptionoftheunweightedandweightedleastsquarephaseunwrappingalgorithm.Thenouralgorithmcombiningwiththeweightedleastsquarephaseunwrappingguidedbythebranch-cutsderivedfromGoldstein’salgorithmandcoherencecoefficientmapderivedfromtheINSARdataisprovided.InourexperimentwewritesubroutinesoftheGoldstein’sbranch-cutalgorithm,unweightedandweightedleastsquarephaseunwrappingalgorithmaswellasouralgorithm,andconstructasmallexperimentsystemtoresolvethephaseunwrappingproblem.FinallywetestouralgorithmonsomeINSARdata.Theresultshowsthatourapproachcanobtainunwrappedphasecorrectlyandefficiently.