EFFECTS OF SHEAR STRESS ON THE VIABILITY PLANT CELLS

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摘要 Plantcelllinesdifferredgreatlyintheabilitytowithstandshearstresses.Usingto-baccocellsandlicoricecellsasmodelplantcells,westudiedtheeffectsofshearstressesonthevi-abilityofplantcells.OurexperimentswerecarriedoutonahighshearrateCouetterheometerprovidinghomogeneousandconstantshearstressesoflaminarflow.TheviabilitywasdeterminedbyTTC(2,3,5-Triphenyltetrazoliumchloride).Theresultswereasfollows.(1)Theviability(V)droppedexponentiallywithtime(t),namelyV=Exp(-kt),(k>oisaconstant).Thismeantthetenabilityofstatisticalhomogeneity.(2)Thevalueofkwasafunctionofplantcells’mechanicalpropertiesandtheshearstressactingontheplantcells.Theshearratecorrespondingtok=owasthecriticalshearratethattheplantcellscouldwithstand.Itcanbeeasilydetermindedbyextrapo-lation.For7-day-oldtobaccocells,itwas1090s-1andfor9-day-oldlicoricecells,itwas6566s-1.(3)Theplantcellsuspensionswerepseudoplasticfluidsfittingτ=Kγn.Forthetobaccocellsus-pensiontested,n=O.73,andforthelicoricecellsuspensiontestedn=0.7.Thusthecriticalshearstressforthetobaccocellswas25dynes/cm2andforthelicoricecellsitwas80dynes/cm2.(4)Oneoftheirreasonsforlicoricecellstohavegreatertolerancetoshearstressesthantobacaccocellsmaybethegeometricfeaturesofthecellsandthesizesofthecells.Thelicoricecellswererod-shaped,butthetobaccocellsweresphericalandlargerthanthelicoricecells.
机构地区 不详
出版日期 1993年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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