STUDY ON THE ULTRASTRUCTURE OF SINGLE HeLa CELL TREATED WITH HpD PLUS LASER MICROIRRADIATION

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摘要 AmethodisdescribedwhichpermitstransmissionelectronmicroscopeofsinglecellstreatedwithHpDpluslasermicroirradiation.Thepreselectedsinglecellthatwasirradiatedbylaserunderlightmicroscopeandfollowedfixation,embeddedandsectioningisexaminedunderelectronmicroscope.Theresultsdemonstratedthatatthelightdoseof1.88ml/μm2notonlytheirradiatednucleolusappearedtransparentregion,buttheotherpartssuchasnon-irradiatedmitochondriaincytoplasmcanalsobedamaged.Whenpartialcytoplasmisirradiatedwiththelightdoseof4.50ml/μm2,thedamagesappearinallcytoplasm,butthereislittlechangeinthenucleus.Theexperimentalresultsalsodemonstratethatcytoplasmismoresensitivethannucleus.ItisthemitochondriaincytoplasmthatareverysensitivetoHpDpluslaser.
机构地区 不详
出版日期 1990年04月14日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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