摘要
Marineorganismsconstituteunlimitedresourceofbioactivesubstancesduetotheirhighbiodiversityandrepresentavaluablesourceofnewcompounds.Thisstudyoptimizedthealkali-extractionconditionsandantioxidantactivitiesofsolublepolysaccharidesfromthebodywallofSipunculusnudus.Theeffectsofsolid-liquidratio,extractionduration,extractiontemperature,andalkaliconcentrationontheyieldofS.nuduspolysaccharides(SNP)wereexamined,accordingtowhichtheoptimalcombinationofextractionparameterswasobtainedbyanorthogonaltest.TherelativeinfluencingimportanceofdifferentextractionparametersontheyieldofSNPfollowedtheorderassolid-liquidratio>extractiontemperature>alkaliconcentration>extractionduration.Thehighestextractionyield,1.98%,wasachievedunderanoptimalextractioncondition:temperature60℃,solid-liquidratio1:6gmL?1,duration5h,andalkali(NaOH)massfraction6%.TheinvitroantioxidantactivitiesexaminationshowedthatextractedSNPunderthisoptimizedconditionhadstrongpowerinreducingcertainhydroxylandsuperoxideradicalscavengingabilities.ThepromisingresultsshowedthatextractedSNPcouldbeapotentnaturalantioxidant.
出版日期
2017年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)