摘要
Sincethegenerationoffull-sibartificialtriploidfamilies,rapidcloneestablishmentandgeneticimprovementshavebeenneeded.Here,wereportaninvitromethodofdirectshootregenerationofatriploidhybridpoplar[(Populussimonii9P.nigra‘Italica’)9(P.9‘popularis’)].Usingdifferentrandomizedblockdesigns,weselectedonetriploidtoevaluatetheexplanttype,optimalconcentrationsofplantgrowthregulatorsandagar,andculturetimeunderlightordarkconditionsover60days.Thehighestrateofshootinduction,80.0%,wasobtainedusingMurashigeandSkoog(MS)mediumsupplementedwith0.2mg/Lbenzyladenine,0.04mg/Lnaphthaleneaceticacid(NAA),and5.5g/Lagarforthefirst30daysinthedark,then3g/Lagarforthenext30daysinlight.Thislastmediumyieldedthebestrateofshootinduction(6.32shoots/explant).Thesethreemediawerealsousedtoevaluatetheinfluenceofthegenotypesoftheparentsandhybridtriploidsonregeneration.Twoparentsandthreeofthefourfull-sibtriploidswereregeneratedsuccessfully;differentgenotypesandexplanttypessignificantlyaffectedtherateofshootinductionandaveragenumberofshoots.Leavesbutnotpetioleswereasuitableexplant.Onegenotypeproducedthehighestrateofshootinductionof96.67%.Half-strengthMSmediumsupplementedwith0.2mg/Lindolebutyricacidand0.04mg/LNAAwasthemosteffectiveforrooting;rootingratewas96.67%,survivalrateoftransplantswas73.33%,androotingfrequencysurpassed85%foreachgenotype.Overall,thisinvitroregenerationsystemwillbeusefulforthepropagationandgeneticmodificationoftriploidpoplars.
出版日期
2018年06月16日（中国期刊网平台首次上网日期，不代表论文的发表时间）
