摘要
Objective:Tofurtherinvestigatetheosteogenicpotentialofrabbitmarrowstromalstemcellsculturedinvitro.Methods:Rabbitmarrowstromalstemcellswereisolatedbydensitygradientcentrifugationmethodandamplifiedintheflasks,usingtheosteogenicinducingconditions(OGC)astheculturemedia.Theosteogenicpotentialofmarrowstromalstemcellswereinvestigatedbymeansofbone-seekingfluorescenc(tetracycline)labeling,AlizarinredS(ARS)staining,Alcianblue-Siriusred(AS)staining,andscanningelectronmicroscope.Results:Afterbeingpassaged,themarrowstromalstemcellsincreasedinnumber,becameconfluentandformedmulti-layerstructure.Thestromalstemcellsexcretedinnumerabletinygranules,heapinguponthecellbodyandmerginggraduallyintofoggysubstances.Thesefoggysubstanceskeptonenlargingandformedround,oval,orflake-likenodules.Thesenodulesrevealedbrightgoldenyellowfluorescenceunderfluorescencemicroscopewhenlabeledwithtetracycline.HistochemicalstudywithspecificnewbonestainingwithARSrevealedpositivecalciumreaction,bothdenotingthattheywerenewlyformedbonetissues.AftertheywerestainedwithAS,collagenandacidmucopolysaccharidewereshown.Underscanningelectronmicroscope,threetypesofcellswithdifferentconfigurationswerefound.Theywereglobularcells,spindle-shapedcellsandpolygonalorpolygonalcells.Granuleswereexcretedfromthecellsandheapeduponthecellbody.Needle-shapedandirregularlyrectangularcrystalsalsoappearedandagglomeratedwiththegranulestoformnodulesandtrabecula-likeorflake-likestructures.Conclusions:Sequenceofeventsofboneformationbyrabbitmarrowstromalstemcellsculturedinvitroisfullydepictedandconfirmed,whichprovidesthefoundationforfurtherinvestigatingthemechanismsofosteoblastdifferentiationfrommarrowstromalstemcellsandthepossibleapplicationinorthopaedics.
出版日期
2002年06月16日（中国期刊网平台首次上网日期，不代表论文的发表时间）
