Detection of Neisseria Gonorrhoeae from Urine with Ligase Chain Reaction-中国期刊网

摘要 Objective:Toevaluatethevalueofligasechainreaction(LCR)inthediagnosisofdiplococcusgonorrhoeaeinurine.Methods:LCRdetectionoftheurineforNeisseriagonorrhoeaeandbacteriacultureofdischargewasper-formedsimultaneouslyto276patientswithurethritisorcervicitisseekingtreatmentinsextransmitteddis-eases(STDs)outpatientclinic.Forspecimenswithdiscordantresults,polymerasechainreactionwasconducted.ThepurposewastodetecttherespectivesensitivityandspecificityofbacteriacultureandLCR.Results:24of276(8.7%)patientshadpositiveLCRresultsand21of276(7.6%)werepositiveforculture.5specimenshaddiscordantresultsfromLCRandbacteriaculture.ThesensitivityandspecificityofLCRinthediagnosisofgonorrhoeaewere92.3%and100%respectively.Conclusion:ThisstudyshowedthatLCRhadahighersensitivityandspecificityforthediagnosisofgonorrhoeaefromurine.

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Detection of Neisseria Gonorrhoeae from Urine with Ligase Chain Reaction

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Detection of Neisseria Gonorrhoeae from Urine with Ligase Chain Reaction

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