Construction of Hybrid Enzyme from HEC-SOD and Cu,Zn-SOD-中国期刊网

摘要 Humanextracellularsuperoxidedismutase(hEC-SOD)isasecretedtetramericproteininvolvedintheprotectionofahumanbodyfromoxygenfreeradicals.Itsthree-dimensionalstructurehasnotbeenconfirmed,hEC-SODcouldn′tbeexpressedinE.coll.Weconstructedahybridenzyme,whichcomprisestheNterminalandC-terminaldomainsfromhEC-SOD,fusedittohumanCu,Zn-SOD.ThehybridenzymeisexpressedsuccessfullyinE.coli.Further,weanalyzedtheexpressionofhEC-SODinE.colibymRNAdifferentialdisplaying.

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Construction of Hybrid Enzyme from HEC-SOD and Cu,Zn-SOD

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Construction of Hybrid Enzyme from HEC-SOD and Cu,Zn-SOD

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