An Experimental Study on the Role of Nuclear Factor-κB in the Signal Conduction of Protein Kinase C Regulating the Proliferation and Apoptosis of T Lymphocytes in Asthma-中国期刊网

摘要 Toexploretheroleofnuclearfactor-κB(NF-κB)inthesignalpathwayofproteinkinaseC(PKC)regulatingtheproliferationandapoptosisofTlymphocytesinasthma.Tlymphocyteswereisolatedfromtheasthmaticmodelofguineapigsandtheasthmaticpatients.EithertheTcellsstimulatedwithPMAaloneorthosestimulatedwithPMAtogetherwithpyrrolidinedithiocarbamate(PDTC)wereincubatedfor1and24h.TheproliferationofandthepresenceofNF-κBinthecellsincubatedfor1hwereobservedbyMTTandimmunohistochemicalstaining,respectivelyAndthecellsincubatedfor24hwereobservedfortheapoptosisbyTUNEL.Alltheassayswereparalleledwithcontrols,andallthedatawereanalyzedstatisticallywiththesoftwareSAS.ThepercentageofcellsofnuclearpositivestainingofNF-scBandtheproliferationofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithPMAweresignificantlyhigherthanthoseofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithoutPMArespectively(P<0.01)andthoseofTlymphocytesfromnormalcontrolguineapigsandnormalcontrolpersonsstimulatedwithPMArespectively(P<0.01),andweresignificantlyreducedbyPDTC(P<0.01).TheapoptosisindexofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithPMAweresignificantlylowerthanthoseofTlymphocytesfromasthmaticguineapigsandasthmaticpatientsstimulatedwithoutPMArespectively(P<0.01)andthoseofTlymphocytesfromnormalcontrolguineapigsandnormalcontrolpersonsstimulatedwithPMArespectively(P<0.01),andweresignificantlyinducedbyPDTC(P<0.01).ThereweregoodpositivecorrelationbetweenthepercentageofcellsofnuclearstainingofNF-κBofTlymphocytesandtheproliferationofTlymphocytes(r=0.51-0.72,P<0.001),andalsogoodnegativecorrelationbetweenthepercentageofcellsofnuclearstainingofNF-scBandtheapoptosisindexofTlymphocytes(r=-0.55-0.71,P

1Cao Ying-Ya,Wang Zhen,Lu Lin-Ming,Xu Zeng-Xiang,Li Jia-Jia,Jiang Xiao-Gan,Lu Wei-Hua. Polo-like kinase 1 protects intestinal epithelial cells from apoptosis during sepsis via the nuclear factor-κB pathway.医药卫生,2020-08.
2Nu ZHANG Heather HARTIG Ivan DZHAGALOV David DRAPER You Wen HE. The role of apoptosis in the development and function of T lymphocytes.细胞生物学,2005-10.
3杨茜;赵桂秋;李倩;刘相萍. Expression of nuclear factor-κB in traumatic cataract.临床医学,2006-02.
4张本静;王焱林;王成天;柯剑娟. Effect of Shenfu injection on nuclear factor-κB during myocardial ischemia／reperfusion injury in rats.临床医学,2005-04.
5GuoBC;XuYU. Bcl－2 over－expression and activation of protein kinase C suppresss the Trail－induced apoptosis in Jurkat T cells.细胞生物学,2001-02.
6Yue Lu;Leng Li;Jin-Wei Zhang;Xiao-Qin Zhong;Jian-An Wei;Ling Han. Total polysaccharides of the Sijunzi decoction attenuate tumor necrosis factor-α-induced damage to the barrier function of a Caco-2 cell monolayer via the nuclear factor-κB-myosin light chain kinase-myosin light chain pathway.消化系统,2018-12.
7Alexey E Granovsky Marsha Rich Rosner. Raf kinase inhibitory protein： a signal transduction modulator and metastasis suppressor.细胞生物学,2008-04.
8Run-Long Lin;Lu-Jun Zhao. Mechanistic basis and clinical relevance of the role of transforming growth factor-β in cancer.肿瘤,2015-04.
9Mu Sheng-Tian,Tang Jie,Ma Jian-Qi,Zhong Yu,Liu Han-Zhe,Ma Xiao-Chun,Zheng Zhen. Unfractionated heparin attenuates endothelial barrier dysfunction via the phosphatidylinositol-3 kinase/serine/threonine kinase/nuclear factor kappa-B pathway.医药卫生,2020-08.
10Liu Yingmei;Fu Yuewu;Wei Yulin;Wu Wei. Experimental Study of 103Pd Stent Affecting Dynamic Equilibrium Between Proliferation and Apoptosis of Vascular Smooth Muscle Cells.心血管疾病,2006-02.

An Experimental Study on the Role of Nuclear Factor-κB in the Signal Conduction of Protein Kinase C Regulating the Proliferation and Apoptosis of T Lymphocytes in Asthma

来源期刊

相关推荐

同分类资源更多

相关关键词

An Experimental Study on the Role of Nuclear Factor-κB in the Signal Conduction of Protein Kinase C Regulating the Proliferation and Apoptosis of T Lymphocytes in Asthma

来源期刊

相关推荐

同分类资源 更多

相关关键词

同分类资源更多