摘要
AIM:Todeterminewhetherischemicpreconditioning(IP)affectsthedevelopmentofedematouscerulein-inducedpancreatitisandtoassesstheroleofcyclooxygenase-1(COX-1),COX-2,andheatshockprotein70(HSP70)inthisprocess.METHODS:InmaleWistarrats,IPwasperformedbydampingofceliacartery(twicefor5minat5-minintervals).ThirtyminutesafterIPorshamoperation,acutepancreatitiswasinducedbycerulein.ActivityofCOX-1orCOX-2wasinhibitedbyresveratrolorrofecoxib,respectively(10mg/kg).RESULTS:IPsignificantlyreducedpancreaticdamageincerulein-inducedpancreatitisasdemonstratedbytheimprovementofpancreashistology,reductioninserumlipaseandpoly-Cribonucleaseactivity,andserumconcentrationofpro-inflammatoryinterleukin(IL)-1β.Also,IPattenuatedthepancreatitis-evokedfallinpancreaticbloodflowandpancreaticDNAsynthesis.Serumlevelofanti-inflammatoryIL-10wasnotaffectedbyIP.Cerulein-inducedpancreatitisandIPincreasedthecontentofHSP70inthepancreas.MaximalincreaseinHSP70wasobservedwhenIPwascombinedwithcerulein-inducedpancreatitis.InhibitionofCOXs,especiallyCOX-2,reducedtheprotectiveeffectofIPinedematouspancreatitis.CONCLUSION:OurresultsindicatethatIPreducespancreaticdamageincerulein-inducedpancreatitisandthiseffect,atleastinpart,dependsontheactivityofCOXsandpancreaticproductionofHSP70.
出版日期
2005年12月22日（中国期刊网平台首次上网日期，不代表论文的发表时间）
