Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces

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摘要 ThispaperreportstheadsorptionofBovineSerumAlbumin(BSA)ontoDielectricBarrierDischarge(DBD)processedPoly(methylmethacrylate)(PMMA)surfacesbyaQuartzCrystalMicrobalancewithDissipationmonitoring(QCM-D)tech-nique.ThepurposeistostudytheinfluenceofDBDprocessingonthenatureandscaleofBSAadsorptiononPMMAsurfaceinvitro.ItwasobservedthatDBDprocessingimprovesthesurfacewettabilityofPMMAfilm,afactattributabletothechangesinsurfacechemistryandtopography.ExposureofthePMMAtoPhosphateBuffedSaline(PBS)solutionintheQCM-Dsystemresultedinsurfaceadsorptionwhichreachesanequilibriumafterabout30minutesforpristinePMMA,and90minutesforprocessedPMMAsurface.SubsequentinjectionofBSAinPBSindicatedthattheproteinisimmediatelyadsorbedontothePMMAsurface.ItwasrevealedthatadsorptionbehaviourofBSAonpristinePMMAdiffersfromthatonprocessedPMMAsurface.AsloweradsorptionkineticswasobservedforpristinePMMAsurface,whilstaquickadsorptionkineticsforprocessedPMMA.Moreover,thedissipationshiftofproteinadsorptionsuggestedthatBSAformsamorerigidstructureonpristinePMMAsurfacethatonprocessedsurface.ThesedatasuggestthatchangesinwettabilityandattendantchemicalpropertiesandsurfacetextureofthePMMAsurfacemayplayasignificantroleinBSAadsorptionprocess.
机构地区 不详
出版日期 2008年03月13日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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