简介：Fluorescenceliftimeimaging(FLIM)ofmodifiedhydrophobicbodipydyesthatactasfluorescentmolecularrotorsshowsthatthefluorescencelifetimeoftheseprobesisafunctionofthemicroviscosityoftheirenvironment.Incubatingcellswiththesedyes,wefindapunctateandcontinuousdistributionofthedyeincells.Theviscosityvalueobtainedinwhatappearstobeendocytoticvesiclesinlivingcellsisaround100timeshigherthanthatofwaterandofcellularcytoplasm.Time-resolvedfluorescenceanisotropymeasurementsalsoyieldrotationalcorrelationtimesconsistentwithlargemicroviscosityvalues.Inthisway,wesuccessfullydevelopapracticalandversatileapproachtomapthemicroviscosityincellsbasedonimagingfluorescentmolecularrotors.
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