简介：Wehaveappliedconceptsfrominformationtheoryforacomparativeanalysisofdonor(gt)andacceptor(ag)splicesiteregionsinthegenesoffivedifferentor-ganismsbycalculatingtheirmutualinformationcontent(relativeentropy)overaselectedblockofnucleotides.Asimilarpatternthattheinformationcontentdecreasesastheblocksizeincreaseswasobservedforbothregionsinalltheorgan-ismsstudied.Thisresultsuggeststhattheinformationrequiredforsplicingmightbecontainedintheconsensusof～6-8ntatbothregions.Weassumefromourstudythateventhoughthenucleotidesareshowingsomedegreesofconservationintheflankingregionsofthesplicesites,certainlevelofvariabilityisstilltolerated,whichleadsthesplicingprocesstooccurnormallyeveniftheextentofbasepairingisnotfullysatisfied.Wealsosuggestthatthisvariabilitycanbecompensatedbyrecognizingdifferentsplicesiteswithdifferentspliceosomalfactors.
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