简介：到米饭异种的分子的水平上的变化的特征在空间环境导致了的进一步的学习，我们在四米饭异种(二high-tillering和二low-tillering)的叶子和种子分析了蛋白质在8(th)并且9(th)在一架15天的空间班机以后的代，并且与他们由two-dimentionalpolyacrylamide的扎根的控制相比，凝胶电泳和颠倒分阶段执行液体层析(RPLC)。另外，变异的种子的白朊，血球素，谷醇溶蛋白，谷蛋白，和直链淀粉被RPLC和紫外线spectrometry分析。结果证明在山峰的叶子的低丰富的蛋白质是到ering舞台为止更可能的与他们的相应控制相比被导致。当时，揭示的变异的种子的白朊，血球素，和谷醇溶蛋白变化稳定地与他们在不同异种的变化的控制，和特征相比被继承在8(th)并且9(th)代，建议他们能被用作简历标记到异种由空间飞行导致了的身份。而且，二蛋白质(SSP9111和SSP6302)被发现在不同异种与高紧张(双重的变化)被表示，它两个都根据集体spectrometry并且数据库寻找与相片系统被相关。

简介：ShortinterferingRNA(siRNA)iswidelyusedforstudyingpost-transcriptionalgenesilencingandholdsgreatpromiseasatoolforbothidentifyingfunctionofnovelgenesandvalidatingdrugtargets.TwosiRNAfragments(siRNA-aand-b),whichweredesignedagainstdifferentspecificareasofcodingregionofthesametargetgreenfluorescentprotein(GFP)gene,wereusedtosilenceGFPexpressioninculturedgfptransgeniccellsofrice(OryzasativaL.;OS),cotton(GossypiumhirsutumL.;GH),Fraserfir[Abiesfraseri(Pursh)Poir;AF],andVirginiapine(PinusvirginianaMill.;PV).DifferentialgenesilencingwasobservedinthebombardedtransgeniccellsbetweentwosiRNAs,andtheseresultswereconsistentwiththeinactivationofGFPconfirmedbylaserscanningmicroscopy,Northernblot,andsiRNAanalysisintestedtransgeniccellcultures.ThesedatasuggestthatsiRNA-mediatedgeneinactivationcanbethesiRNAspecificindifferentplantspecies.TheseresultsindicatethatsiRNAisahighlyspecifictoolfortargetedgeneknockdownandforestablishingsiRNA-mediatedgenesilencing,whichcouldbeareliableapproachforlarge-scalescreeningofgenefunctionanddrugtargetvalidation.