简介：Onthebasisofthetemperatureobservationsduring1961-2000inChina,sevencoupledgeneralcirculationmodels'(GCMs)extremetemperatureproductsareevaluatedsuppliedbytheIntergovernmentalPanelonClimateChange's4thAssessmentReport(IPCC-AR4).Theextremetemperatureindicesinusearefrostdays(FD),growingseasonlength(GSL),extremetemperaturerange(ETR),warmnights(TN90),andheatwavedurationindex(HWDI).Resultsindicatethatallthesevenmodelsarecapableofsimulatingspatialandtemporalvariationsintemperaturecharacteristics,andtheirensembleactsmorereliablethananysingleone.Amongthesevenmodels,GFDL-CM2.0andMIROC3.2performancesaremuchbetter.Besides,mostofthemodelsareabletopresentlineartrendsofthesamepositive/negativesignsastheobservationsbutforweakerintensities.Thesimulationeffectsaredifferentonanationwidebasis,with110°Nasthedivision,east(west)ofwhichtheeffectsarebetter(worse)andthepoorerovertheQinghai-TibetanPlateauinChina.Thepredictionsforthe21stcenturyonemissionsscenariosshowthatexceptdecreasesintheFDandETR,otherindicesdisplaysignificantincreasingtrend,especiallyfortheindicesofHWDIandTN90,whichrepresentthenotableextremeclimate.Thisindicatesthatthetemperature-relatedclimateismovingtowardstheextreme.Inthelate21stcentury,theGSLandTN90(HWDI)increasemostnotablyinsouthwestChina(theQinghai-TibetanPlateau),andtheFDdecreasemostremarkablyintheQinghai-TibetanPlateau,northwestandnortheastofChina.ApartfromSouthChina,theyearlychangerangeoftheextremetemperatureisreducedinmostofChina.

简介：Inthisstudy,threecDNAsequencescorrespondingtocytochromeP450C17(CYP17I),3β-hydroxysteroiddehydrogenase(3β-HSD)andandrogenreceptor(AR)wereisolatedfromspottedseabass(Lateolabraxmaculatus).ThemRNAabundancesofCYP17Iand3β-HSDincreasedfromstageⅡtostageⅤwithasignificantincreaseatstageⅤ,andthehighestabundanceofARmRNAwasdetectedatstageⅢintesticulardevelopmentcycle.CYP17I,3β-HSDandARtranscriptswereobviouslyabundantinsteroidogenesistissuessuchastestis,brain,headkidneyamongothers.StrongandpositivesignalswereobservedmainlyininterstitialcellregionsofL.maculatustestisasweremeasuredwithinsituhybridizationmethod.SignificantincreasesofCYP17Iand3β-HSDtranscriptsweredetectedafter12–48hhCG(humanchorionicgonadotropin)andGnRHa(gonadotropin-releasinghormoneanalogue)treatments.However,anoppositerelationshipwasfoundforARintestisatthesametime.Inaddition,decreasingtrendsofCYP17Iand3β-HSDmRNAwereobservedintestisofL.maculatusinfreshwatergroup(FW)fromday2today6,andmRNAabundanceofARincreasedinbrackishwater(BW)groupfromday4today8.Thesefindingsrevealedthatthesethreesteroidsynthesisgenesareimportfortesticulardevelopment,hormoneandsalinitytreatment,andprovidedalsoaninsightintothemechanismofreproductiveendocrineofL.maculatus.