简介：TheaimofthisstudyistoexplorethegenomicmolecularorganizationandgenogroupofhumannorovirusfrominfectedinfantsinGuangzhouofChina.PrimersweredesignedaccordingtothegenomicsequenceofnorovirusintheGenBank,andthenorovirusgenomewasamplifiedbyRT-PCR.ThePCR-productswereclonedintoTvectorandsequenced,andthegenomicnucleotidesequenceswereanalyzedwiththeprogramsCLUSTALW/X,DNASTARandRAT(RecombinationAnalysisTool).TheNVgz01straingenomeis7558bpinlengthandencodesthreeopenreadingframes(GenBankaccessionNo.isDQ369797).ThegenomicsequencesofNVgz01werecomparedwiththoseofnomvirusinGenBank,whichrevealedthatthehomologywithgenogroupⅡrangesbetween76%-90%,andgenogroupⅠbe-tween43%-44%.TheORF1regionshared94%and88%identitywithMc37andFarmingtonstrains,respectively;thecapsidregion(ORF2)shared65%and94%identitywithMc37andFarmingtonstrains,respectively.Phylogenetictreeswerereconstructedbytheneighbor-joiningmethod.ComparativecompletesequenceanalysisoftheNVgz01withreportedhumannorovimsgenomicsequencesrevealedthatthisisolatebelongstogenogmupⅡ.TheORF1andORF2regionsshareddifferentidentitywithMc37andFarmingtonstrains,suggestingNVgz01couldbearecombinantvires.

简介：Wehavepreviouslydemonstratedtheabilityofmalariaparasitestointerferewithspecificimmuneresponses.CD4Tcellsspecifictoparasiteantigens,butnotCD4Tcellsspecifictoanirrelevantantigen,ovalbumin(OVA),aredeletedviaapoptosisduringmalariainfection.Itisofinterest,therefore,toinvestigatetheimmuneresponsesthatdevelopedfollowingvaccinationwiththe19kDacarboxylterminusofthemerozoitesurfaceprotein1(MSP119)inmicethathadpreviouslyexperiencedmalariainfection.Inthisstudy,pre-exposureofmicetoPlasmodiumyoeliielicitednativeanti-MSP119antibodyresponses,whichcouldbeboostedbyvaccinationwithrecombinantMSP119，Likewise,infectionofMSP119-primedmicewithPlasmodiumyoelii(P.yoelii)ledtoanincreaseofanti-MSP119antibodies.MSP119vaccinationofmalariapreexposedmiceorimmunizationbyinfection/cureofMSP119-primedmiceenabledthemicetosurvivechallengeinfection,withtheformergrouphavingslightlylowerparasitaemia.Thedatasuggestthatexposuretomalariainfectionprimesanaturalimmuneresponsewhichcanbeboostedbyvaccination.Thisinformationisrelevanttothedevelopmentofavaccineforuseinindividualslivinginmalaria-endemicareas.

简介：ToanalyzethegenomicmolecularstructureandgenotypeofhumanastrovirusisolatedfrominfantinGuangzhouofChina,theprimersweredesignedbasedonthegenomicsequenceofastrovirusfromtheGenBankandthetargetsequencewereamplifiedbyRT-PCR.ThenthePCR-productswereclonedtoTvectorandsequenced.ThegenomicnucleotidesequenceswereanalyzedbytheprogramsCLUSTALWandDNASTAR.ItwasfoundthatthefullgenomiclengthofHASTVgz01strainwas6721bpandtheORFswere6558bp.The5'and3'UTRwere82and81nucleotides.Thegenomeincluded3openreadingframes(ORFs):ORF1a,ORF1bandORF2.The5'-terminalORF1astartedatnucleotide83andextendedtonucleotide2845.ORFlb(nt2785tont4332)overlapedORFlaby61nucleotides.The3'-terminalORF2beganatnucleotide4325andterminatedatnucleotide6640.ORF2had2316nucleotides.ComparedwithotherastrovirussequencesinGenBank,thehomologyoftheaminoacidsequenceofORF2ofHASTVgz01strainwiththatofserotype4was93%.Homologywithotherserotypesrangedfrom61%to70%.ThecompletenucleotidesequenceofastrovirusHASTVgz01strainisolatedfromGuangzhouinChinawas6721bpinlength,GenBankaccessionNO.DQ344027.ComparingtheORF2ofastrovirusHASTVgz01withtheknownsequencesoftypes1-8thehighesthomologywasserotype4(93%).ComparativesequenceanalysisoftheHASTVgz01ORF2withthereportedhumanastrovirussequencesrevealedthattheisolatedastrovirusbelongstogenotype(serotype)4.

简介：Toinvestigatethematernal-infantileinfectionwithhumanparvovirusB19,theIgGandIgMantibodiesagainsthumanparvovirusandtheB19-DNAinserumandperipheralbloodmononuclearcells(PBMC)ofpregnantwomenaswellastheserumIgMantibodyagainstB19andtheB19-DNAinserumandcordbloodnucleatedcells(CBNC)ofnewbornsweredeterminedbyELISAandnestedPCRrespectively.ItwasfoundthatthepositiverateoftheIgGantibodyagainsthumanparvovirusB19inseraof92pregnantwomenwas38.04%(35/92),andthatoftheIgMantibodyin720pregnantwomenwas9.03%(65/720).However,theIgMantibodyagainsthumanparvovirasB19wasnegativeinthecordbloodseraof95newborns.AstothehumanparvovirasB19DNA,noneof720pregnantwomenand95newbornswasprovedtobepositiveintheirsera,Nevertheless,thepositiverateoftheparvovirasB19DNAinPBMCwas3.06%(3/98)in98pregnantwomenand1.12%(1/89)inCBNCof89newborns.ItisconcludedthatthehistoryofinfectionwithhumanparvovirasB19existsincertainpregnantwomenwithasmallpercentageofpregnantwomeninfectedwithrecentoracuteinfectionsofB19virus.ThedetectionratesoftheB19viralDNAinPBMCofpregnantwomenandCBNCofnewbornswerehigherthanthoseinsera,indicatingthattheriskforverticaltransmissionisverylow.

简介：【摘要】自2019年12月武汉发现不明原因肺炎以来，随着疫情的迅速蔓延，我国其他地区及境外也相继发现此类病例，该疫情发展成为影响人类健康的公共卫生事件；新型冠状病毒肺炎疫情会给患者带来不良心理情绪，因此，针对方舱医院轻症患者在治疗疾病的同时不可忽略心理护理问题，有效进行患者心理护理，有利于提高患者救治率。

简介：

简介：【摘要】目的 分析肥东县梅毒的流行病学特征，为制定梅毒防控措施提供重要的科学依据。 方法 流行病学“三间”分析的研究方法。 结果  2015-2020年肥东县共报告3239例梅毒，梅毒发病率总体呈上升趋势，六年发病率升幅为99.86%，六年各年度间报告发病率差异有统计学意义（χ2=213.904，P0.001）；六年报告数居前六位的职业依次为农民、家务及待业、商业服务、离退人员、工人、其他，六种职业共报告病例3125例，占梅毒报告总数的96.48%；六年的地区分布中，居前十位的依次为店埠镇、撮镇镇、梁园镇、白龙镇、八斗镇、古城镇、石塘镇、长临河镇、牌坊乡、众兴乡，前十位总数为2531例，占六年发病总数的78.14%。 结论 2015-2020年肥东县梅毒发病率上升明显，升幅高达99.86%，说明我县梅毒疫情形势在较长时间内依然十分严峻，必需继续加大加强我县梅毒的防治工作，尤其要加强对高危人群的健康教育重点干预工作，即加强对梅毒性病防治知识的宣传，从而有效遏制梅毒的高流行状态。

简介：摘要：目的 通过分析2015-2020年丽江市疫苗接种后疑似预防接种异常反应（AEFI）的发生特征，评估该市AEFI监测系统的运转状况及预防接种安全性。方法 收集2015-2020年所有疫苗AEFI报告个案和接种剂次数，采用描述性流行病学方法分析AEFI发生率。结果 全市报告发生AEFI的疫苗有27种，共报告个案818例，年均报告发生率为32.38/10万剂，以一般反应为主，无接种事故、疫苗质量事故报告。DTaP、MR、MPV-A疫苗AEFI报告病例数较多，非免疫规划疫苗报告发生率居高。男性发病高于女性，1岁内儿童报告数较多，发病集中在夏秋季。结论 全市AEFI监测系统运行良好，监测敏感性较高，但仍有进一步提升空间。接种安全性较高，以一般反应为主，严重异常反应偶有发生，应加强对监护人接种知识宣教和接种人员业务培训，以此减少AEFI发生情况。

简介：[摘要 ] 目的：了解病毒性肝炎流行规律，为预防控制决策提供依据。方法：流行病学分析。结果：五年平均发病率为 40.03/十万，居法定报告传染病的第二位，男性（ 454例）高于女性（ 254例）；甲型肝炎、乙型肝炎和其他肝炎（丙、丁、戊型和未分型病毒性肝炎，以下同）年均发病率分别为 2.96/十万、 32.22/十万和 4.86/十万，无丁型病毒性肝炎病例报告。结论：病毒性肝炎为昌宁县法定报告传染病的主要病种，呈现一定的季节性、地区性和病人群分类性分布特点。

简介：【摘 要】医院建立在“实事求是”基础上制度设计，合理性、有效性的流程，不但满足质量保证的需要，又兼顾到质量控制。医院感染监测系统利用信息技术主动发现传染病患者实时监控，提高了新冠肺炎疫情报告的及时性和疫情监测数据的准确性。