简介：ToselecttheimmunogenicshortpeptidemimicsofmalewormoriginofSchistosomajaponicum(Sj)andtoexploretheirprotectioneffectagainstschistosomiasisinmice,therandomphagedisplaypeptidehbraryof12-merwasscreenedwithIgGtosolublemalewormantigenofSj,andthespecificpositiveclonesselectedthroughthreeroundsofscreeningsweredetectedbyDot-ELISA,andtheninjectedsubcutaneouslyintomiceforvaccinationandprotectionassessmentagainstSj.Itwasfoundthat18randomlypickedphagedisplayedclonesallshoweddefiniteantigenicitywithvariousintensities.Thepooledphagesdisplayedclonescouldinduceproductionofspecificantibodiesandcause31.72%ofwormreductionrateand51.54%ofeggreductionrateinmice,revealingasignificantdifference(P<0.001)incomparisonwiththoseofthecontrols.ItconcludesthattheshortpeptidemimicsofmalewormoriginofSjobtainedbyaffinityscreeningphagedisplayptidelibrarycanelicitpartialprotectionagainstthispathogen.