简介:IntheapplicationofRNAitechnology,itisanessentialsteptodesignsiRNAapplicabletotargetgene.Atpresent,therearemanyresearchesandconclusionsonsiRNAdesign.ThispaperaimstotheinfluencesofmRNAsecondarystructureorsiRNAantisense-strandsecondarystructureonsiRNAsilenceefficiency.Thepaperalsodiscussestheproblemsandsetsoutfurtherinsightsintheresearch.