简介：Objective:ThisstudyexaminedHerpesSimplexVirus(HSV)subclinicalsheddinginthegenitaltractofpatientswithgenitalherpes(GH)ornon-gonoccalurethritis(NGU).Method:SwabswerecollectedafterexposuretorashandgenitaltractduringGHrelapseorremissiononaweeklybasisforfourtosixweeks.NGUpatientswithnegativechlamydiaandmycoplasmatestswerealsoswabbedforasimilarduration.AllswabsunderwentHSVDNAdetectionwithquantitativePCR.Result:TherewasasignificantdifferenceintherateofasymptomaticHSVsheddinginurinarytractscomparingGHandthecontrolgroupandcomparingNGUandthecontrolgroup(P<0.05).TherateofHSVsheddingwas22%,9.8%and3.3%forGH,NGUandcontrolgroupsrespectively.TherateofHSVsheddingwas21.7%(20/92)forpatientswithactiveGHand23%forthoseinremission.TheHSVpositiveratewassignificantlyhigherinthegroupwithpatientswhohadmorethansixrelapseswithinoneyearcomparedtothegroupofpatientswithlessthansixGHrelapses.Conclusion:ThereisHSVsubclinicalsheddingintheirgenitaltractduringactiveGHandremission.SubclinicalHSVsheddingismorecommoninpatientswithmorethansixGHrelapsesperyearcomparedtoGHpatientswithfewerrelapses.Approximately9.9%ofNGUpatientswithnegativechlamydia,mycoplasmatestingwasfoundtohavesubclinicalHSVinfection.

简介：Toinvestigatetheetiologyofgenitalulcerdisease(GUD)amongpatientsattendingsexuallytransmitteddisease(STD)clinicsinGuangzhou,China.BetweenSeptember8,1998,andAugust9,assessed.ClinicaletiologyofGUDwasdependentonincludingthefollowing:darkfieldmicroscopyandserologyforTreponemapallidum(TP),swabsofgenitalulcerforsimultaneousdetectionofHSV,TP,Haemophiusducreyi(HD),Humanpapillomavirus(HPV),andserologyforHIVinfection.Twohundredthirtymenandthirty-sevenwomenwithamedianageof33.4(range16-74years)wereanalyzed.TheetiologyofGUDwassyphilis(26.59%)(71/267),genitalherpes(17.60%)(47/267),condylomataacuminata(4.87%)(13/267),candidiasis(3.37%)(10/267),andmultipeinfection(6.74%)(18/267).TheseroprevalenceofHIVwas0.07%(2/267).Noetiologywasidentifiedin50.56%(135/267).TheetiologyofGUDamongSTDpatientsinourareawasmultifactorialwithapredominanceofsyphilisandgenitalherpes.BasedonthislimiteddataobtainedatSTDclinics,HIVinfectionwasnotcommon.

简介：Objective:Toinvestigatethedepressionstatusofpatientswithsexuallytransmitteddiseases(STDs).Methods:Thedepressionstatusoffifty-onehospitalizedSTDpatientswasevaluatedinarandomizedcontrolstudyusingZung'sQuantitativeTable.18healthycontrolpatientswithsimilardemographicbackgroundswererandomlychosenascontrols.Patientswithscoresaboveorequalto40wereconsideredtobesufferingfromdepression.Results:Theprevalencerateofdepressioninthepatientgroupwasobviouslyhigherthanthatofinthecontrol(X2=16.456,P<0.01).Prevalenceofdepressionwasfoundtobesignificantlyrelatedtooccupation(P<0.05).Thoughtheprevalencewasnotfoundtodiffersignificantlybetweenthosewithatreatmentcourselessthan2monthsandthosewithonelongerorequalto2months(X2=0.041,P>0.05),themeandepressionscoresoftheformergroupweresignificantlyhigherthanthoseofthelatter(P<0.01).Nosignificantdifferenceswerefoundbetweennewversusrelapsingdisease,marriedversusnon-married,maleversusfemale,ordifferingeducationalbackgrounds.

简介：Objective:Todetectandquantitategenitalherpessimplexvirus(HSV)DNAinspecimensfrom100patientsclinicallydiagnosedwithgenitalherpes.Methods:PolymeraseChainReaction(PCR)andenzyme-linkedimmunosorbentassay(ELISA)wereusedwithastandardcurveofDNAcopiesofHSVasquantitativecontrast.Results:Ninety-threecaseswereconfirmedHSVpositiveand7caseswerefoundtobenegative.Therewere58casesofHSV-2(62.4%)and35casesofHSV-1(37.6%)amongthe93positivecases.ThenumberofDNAplasmidsrangedfrom115to1.1×l0^5per250pLamongthe93positivesamples(mean=7.1×10^4/250μL).ThenumberofHSVDNAplasmidsrangedfrom136to1.1×l0^5copiesper250pL(mean=7.6×10^4)amongthosewithHSV-2,and115to9.4×10^4per250pL(mean=6.3×10^4)amongthosewithHSV-1.Meanwhile10μLofextractedanddissolvedDNArandomlytakenfrom8eachofHSV-2andHSV-1samplesweretested.ThenumberofHSV-2DNAplasmidsrangedfrom35copiesto2.7×10^4(Mean=l.8×10^4)andthenumberofHSV-1DNArangedfrom29to2.5×10^4(Mean=1.6×10^4).Inthe7negativecases,thequantityofHSVplasmidswaszero.Conclusion:ThesensitivityofELISAquantitation(93%)isequaltothatofSouthernblot.ThesensitivityofPCRfordiagnosisis91%,and88%forPCRtyping.

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简介：Objective:ToevaluatetheclinicalapplicationofmultiplexPCRinthedetectionofTreponemapallidum,Herpessimplexvirus(HSV),andHaemophilusducreyi.Method:ThreestandardstrainswereusedtosetupamultiplexPCR(MPCR)fordetectingsyphilis,herpesgenitalis,andchancroidsimultaneously.Samplesfrom122patientswithgenitalulcerdisease(GUD)weresubjectedtoMPCRandtheresultswerecomparedwiththeseofdark-fiddmicroscopyandTPserology,HSVanligenELISA,andH.ducreyiculture,Result:Inthe122patientswithGUD,MPCRidentified34casesofT.palliduminfection,40casesofHSVinfection,and2casesofmixedinfectionofT.pallidumandherpes.NopositiveresultsofH.ducreyiwerefound.ThesensitivityofMPCRtoT.pallidumandherpeswas100%and93.3%,respectivdy.Thesensitivitiesofdark-fieldmicroscopyandTPserology,HSVantigenELISA,andH.ducreyiculturewas35.3%,50%and100%,respectively.Conclusion:MPCRshowedarelativelyhighersensitivityforT.pallidumascomparedwiththeroutinetechniques.AlthoughitssensitivityforHSVwasnotasgoodasthatofantigenELISA,italsoyieldedahighdetectionrate.MPCRcandetectmorethanonepathogen.Itissimple,quick,sensitive,andsuitableforclinicaluseorepidemiologicalinvestigation.

简介：Objective:ToanalyzeHIVreplicationandimmunefunctionchangesamongChinesepatientswithHIV/ARC/AIDSandtheirassociationwithdiseaseprogression.Methods:Tcellsubsets,plasmacytokineconcentrationsandviralloadsfrom42HIV+individuals,and13ARC/AIDSpatients,andI0controlswereanalyzedbyflowcytometry(FCM),quantitativeELISAandreversetranscription-polymerasechainreaction(RT-PCR),respectively.Results:CD4cellcountsandplasmaIL-2inHIV/AIDSpatientsweresignificantlylessthaninnormalcontrolsubjects(P<0.001).TheplasmaconcentrationsslL-2R,TNF-a,andNeopterinincreasedsignificantlywithdecreasingCD4cellcounts.PlasmaIL-2amongAIDS/ARCpatientswasalsolessthaninHIV+patients(P<0.01).CD4cellcounts,theratioofCD4toCD8andplasmaIL-2levelsdecreasedsignificantlywithinfectionduration.CD4cellcountsdeclinedanaverageof43/miperyear.Incontrast,theconcentrationofplasmaslL-2R,sTNFR-I,andNeopterinincreasedanaverageof9.03pg/ml,8.69pg/ml,2.11ng/mlperyear,respectively.Furthermore,asignificantreverselinearcorrelationwasobservedbetweenCD4cellcount,CD4/CD8ratio,andCD3,CD4andCD8countswithplasmalevelsofsTNFR-I,Neopterin,andHIVRNAload.ApositivelinearcorrelationwasnotedbetweenplasmasIL-2RlevelsandchangesofplasmaIL-6level(P<0.001),IL-10(P<0.001),TNF-a(P<0.001),sTNFR-I(P<0.005),andNeopterin(P<0.002)andbetweenIL-6andTNF-a(P<0.001),NeopterinandIL-10(P<0.05),sTNFR-I(P<0.001),plasmaviralloadandsTNFR-I(P<0.001),andNeopterin(P<0.002).Conclusion:ThesefindingssuggestacloserelationshipbetweenIL-6andTNF-almmuneactivation,HIVreplicationanddiseaseprogressioninprimaryHIVinfectionsandAIDSpatients.DecliningCD4cellsandplasmaIL-2,andincreasingviralload,sIL-2R,TNF-a,sTNFR-I,andNeopterinmightbeconsideredasimportantpredictorsoftheprogressionofHIVinfectiontoAIDS.

简介：在皮肤病、性病、微创治疗及皮肤美容等方面各位专家的大力支持下,在广大读者关爱下,《皮肤科学通报》,完成了2018年1~6期各专刊的出版工作,2019年依旧就临床医师关注的热点和难点进行讨论和分析。

简介：Objectives:TodevelopamethodofsimultaneousPCRdetectionofHaemophilusducreyi,Treponemapallidum,andHerpesSimplexVirusTypes1and2fromgenitalulcersamongpatientsattendingSTDclinicsinGuangzhou,China;andevaluatetheclinicalapplicationofmultiplexPCR(M-PCR)assayfordiagnosingtheetiologyofgenitalulcerdiseases(GUD).Methods:244patientswithagenitalulcerwereevaluated.ClinicaletiologyofGUDwasbasedonphysicalappearanceandmicrobiologicevaluationsthatincludeddarkfieldmicroscopyexamination(D-F)andserologytestforsyphilis(STS).SwabsofeachgenitalulcerweretestedforHSVantigenbyenzymeimmunoassay(EIA)andprocessedinanM-PCRassayforsimultaneousdetectionofT.pallidum,HSVandH.ducreyi.Results:ThestandardstrainsofT.pallidum,HSVandH.ducreyiwereamplifiedbyM-PCR,producingamplifiedproductsof260bp,432bp,170bp,respectively.ThesensitivityofM-PCRis102pgDNA.M-PCRassayforT.pallidum,HSVandH.ducreyishowedgoodagreementwhencomparedwithD-FdetectionforT.pallidum,STS,H.ducreyicultureandEIAforHSVantigen(Kappascoresare0.774,0.704,0.793,0.756,respectively).Conclusions:TheM-PCRisaconvenient,accurateandreliableassayforthedetectionofT.pallidum,HSVandH.ducreyifromgenitalulcers,andcanbeusedasamethodofdiagnosingtheetiologyofGUD.

简介：经中国中西医结合学会批准,中国中西医结合学会皮肤性病专业委员会定于2019年3月28日—4月1日在江苏省江阴市华西村龙希国际大酒店召开全国中西医结合皮肤性病学术年会。此次会议将发扬历次年会的优良传统,注重中西医结合治疗皮肤病的新方法及新的研究进展等方面的学术交流,内容密切联系临床,切合皮肤科医师的实际需求,会议将邀请知名专家做特邀演讲,阐述皮肤科相关领域的最新研究进展,创造形式多样、内容充实、紧张热烈、活跃互动的学术交流形式,达到全国皮肤科中医、西医、中西医结合医师共同展现才华、获取知识和信息、增进友谊的目的,欲参加会议者请仔细阅读本通知并在规定的时间按要求投稿。