简介:TheSOD-likeactivityoffiveCu(Ⅱ)complexes[Cu(HSal)_2·EtOH,Cu(Gly)_2,Cu(Lys)_2,Cu(His)_2,Cu(Try)_2]havebeenstudiedbyusingcytochromeCmethod,aswellaschemilumine-scenceandESRtechnique.Thefoursurfactants(CTAB,SDS,TritonX-100andTween20)werepurifiedandtheircriticalmiceileconcentration(CMC)inphosphatebuffer(pH=7.4)wasmea-sured.TheeffectsoffoursurfactantsonO_2~-havebeeninvestigated.Theco-operativeeffectsof
简介:TheSOD-likeactivitiesofcoppersalicylateencapsulatedinneutralpositivelyandnegativelychargedliposomeswerestudiedwithcytochromecmethod.TheresultsshowedthattheSOD-likeactivityofcoppersalicylateencapsulatedinneutralliposomeswasenhancedover3timesmorethanthatofcoppersalicylateinaqueoussolution.However,theactivitydecreasedforcoppersalicylateencapsulatedinpositivelyandnegativelychargedliposomes.Itwasalsofoundthatatexperimentalconcentrationstherewasnoregularityforthechangeofactivityofcoppersalicylateencapsulatedinnegativelychargedliposomes,andcoppersalicylateencapsulatedinliposomeswithmorenegativechargescouldpromotethedismutationofsuperoxideanionfreeradicals,however,socouldcoppersalicylateencapsulatedinliposomeswithfewernegativechargesonlyinlowerconcentrations.