简介：Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

简介：以便为对米饭识别米饭变化的抵抗保存病毒黑条纹的矮子疾病，一个简单、可靠的方法被开发，通过哪个没有病毒的小棕色的planthopper(SBPH)获得了米饭从冻结的感染的叶子的黑条纹的矮子病毒(RBSDV)和病毒被传给健康米饭植物。试验性的结果证明SBPH能从冻结的感染的米饭叶子获得RBSDV，病毒能被传给一个易受影响的米饭变化。能力获得RBSDV并且由SBPH把病毒传给健康植物，冻结的感染的叶子之间并且在vitro没有重要差别感染的叶子。新奇方法能被用于米饭变化抵抗的鉴定到米饭黑条纹的矮子疾病，为米饭便于繁殖过程黑条纹的矮子疾病抵抗。