简介:Phenotypical,cytological和到真菌Magnaporthegrisea的米饭的分子的回答用米饭栽培变种和损害被学习模仿植物。栽培变种Katy产生几剧毒的M。grisea孤立,并且Sekiguchi相似损害模仿到这些的提高的抵抗孤立的显示出的Katy(LmmKaty)的异种。损害模仿LmmKaty的显型被剧毒的M很快导致。grisea孤立或在仅仅在接种物的高水平的无毒害的。Autofluorescence(活跃防卫回答的一个符号)在紫外光下面是可见的在在不兼容的相互作用的局部性的接种以后的24h,而,不是在兼容相互作用明显。Autofluorescence也在病原体接种以后在LmmKaty20h被观察,显示快速的房间死亡是限制病原体侵略的LmmKaty的机制。联系的防卫(医生)的快速的累积基因抄本,本氨基丙酸氨lyase并且?-glucanase,被观察在6h开始并且在在一个不兼容的相互作用的接种以后在16h和24h是明显的。PR-1和chitinase的快速的抄本累积在一个不兼容的相互作用在接种以后由24h发生了。这些抄本的累积在兼容相互作用被推迟。这些结果显示那位主人活跃防卫回答发生在病原体接种和那LmmKaty以后的24h展出提高的抵抗到M。grisea。在重接种以后的DR基因的autofluorescence和表示为对M的主人反应的早决心分别地是重要cytological和分子的标记,这被建议。在米饭强风系统的grisea。
简介:Thetransgenicrice,Zhongda2,whichwasgeneticallymodifiedfromanindicaricelineZhuxianBbyricechitinasegene(RC24),hadhighresistancetoricesheathblight(Rhizoctoniasolam)inlaboratoryandatwo-yearfieldexperiment.ThepathogencouldinvadesheathofZhongda2andinducesymptomsofthedisease.NodifferencewasnotedintimeofpenetrationorincubationperiodbetweenZhongda2andnon-transgenicricecontrol,ZhuxianB,butthehyphaelysatecouldbeobservedeadierthancontrol.Itsresistanceexpressedastoinhibitthegrowthofmyceliuminhosttissue.F1sfromZhongda2(♂)crossedwithotherfivenon-transgenicricelinesshowedhigherresistancethandonornon-transgenicparents,buttheresistancewasdifferentalongwiththedifferentmaternalparents.
简介:Twonewlybredhybridricecombinations,superhigh-yieldingLiangyoupeijiu(Pei'ai64S×9311)andPei'ai64S/E32(Pei'ai64S×E32)wereusedtoinvestigatethephotosyntheticcharacteristicsunderhightemperatureincomparisonwithhybridriceShangyou63.Hightemperaturecausedadecreasedphotosyntheticefficiencyandaggravatedphotoinhibition.TheoptimumtemperatureforphotosyntheticelectrontransportationandphotosyntheticCO2fixationwereabout28℃and35-40℃respectively.Linearelectrontransportationismoresensitivetohightemperaturethanthephotochemicalprocess.Themechanismofhightemperatureadaptationwaspossiblyasfollows:superhigh-yieldingricehasquicklyincreasingcarotenoid,whichactedasamorefavorableantioxidantsystemtoreducetheactiveoxygenproductionandavoiddamagetothephotosynthesissystem;superhigh-yieldingricehasahigherefficiencyofxanthophyllscycletodissipateexcessheatenergy;superhigh-yieldingricehasamorestablephotosyntheticfunction,higherphotosyntheticefficiencyandmoreheatstableproteincontent.
简介:Thepromoterregionofadroughtandabscisicacid(ABA)induciblegene,osr40c1,wasisolatedfromasalt-tolerantindicaricevarietyPokkali,whichis670bpupstreamoftheputativetranslationstartcodon.Insilicopromoteranalysisofresultedsequenceshowedthatatleast15typesofputativemotifsweredistributedwithinthesequence,includingtwotypesofcommonpromoterelements,TATAandCAATboxes.Additionally,severalputativecis-acingregulatoryelementswhichmaybeinvolvedinregulationofosr40c1expressionunderdifferentconditionswerefoundinthe5′-upstreamregionofosr40c1.TheseareABA-responsiveelement,light-responsiveelements(ATCT-motif,BoxI,G-box,GT1-motif,Gap-boxandSp1),myeloblastosisoncogeneresponseelement(CCAAT-box),auxinresponsiveelement(TGA-element),gibberellin-responsiveelement(GARE-motif)andfungal-elicitorresponsiveelements(BoxEandBox-W1).Aputativeregulatoryelement,requiredforendosperm-specificpatternofgeneexpressiondesignatedasSkn-1motif,wasalsodetectedinthePokkaliosr40c1promoterregion.Inconclusion,thebioinformaticanalysisofosr40c1promoterregionisolatedfromindicaricevarietyPokkaliledtotheidentificationofseveralimportantstress-responsivecisactingregulatoryelements,andtherefore,theisolatedpromotersequencecouldbeemployedinricegenetictransformationtomediateexpressionofabioticstressinducedgenes.
简介:以便揭示在二CCDD染色体种,Oryzaalta和Oryzalatifolia之间的起源和进化关系,在situ杂交(鱼)的荧光被采用从O与C0t-1DNA分析二种的染色体。alta作为一根探针。Karyotype比较地也在O之间被分析。alta和O。latifolia基于他们杂交的类似的乐队模式发信号。在O之间有高相同和靠近的关系。alta和O。然而,在杂交之间的区别表明的latifolia也是清楚的。C0t-1DNA被证明是种类--并且染色体类型特定。C0t-1DNA鱼能是更有效的分析在不同种类之间的genomic关系,这被建议。根据在二allotetraploidy种之间的高度并且中等重复的DNA序列的比较分析,O。alta和O。latifolia,可能的起源和Oryza的allotetraploidy的进化机制被讨论。
简介:在米饭restorer线C224的条纹疾病抵抗的继承为量的特点加多基因用主要基因的混合效果模型被分析。另外,抵抗与维护者线在C224的七个十字被调查。结果证明C224的条纹抵抗被二主要基因与添加剂优势效果(E-1模型)加多基因与additive-dominance-epistasis效果控制。这二基因有12.47%和24.75%分别地,出现的否定优势效果的添加剂效果。在二主要基因之间有重要epistasis和相互作用效果。当多基因的是2.74%时,二主要基因的可遗传性是92.12%,显示条纹抵抗有主导的主要基因效果。七个十字,五显示了高或中等的抵抗到条纹疾病。