简介：我们识别了米饭花的机关发展异种，称为的同样开的壳和男性无菌1(ohms1)，从indicarestorer线的子孙，Zhonghui8015与60Co光线照耀对待。ohms1异种展出了开的壳并且像词根并且象palea一样组织在花药和耻辱之间的变换，它导致了显示出‘tridentatelemma’的ohms1异种小穗状花小穗。ohms1异种是完全无菌却有的60%-70%肥沃的花粉。印射的基因分析和基因证明ohms1被单个后退的基因控制，并且变异的基因对在标记KY2和KY29之间的染色体3的短手臂上的42-kb间隔印射罚款。在这个区域的四个开的读物框架的顺序分析表明异种在第五intron的最后底带了单个核苷酸转变(到G的A)，它多半被通信到ohms1phynotype，在一种MIKC类型疯盒子的基因OsMADS1(LOC_Os03g11614)。进一步定序的酶消化和cDNA显示可变拼接在MADS领域或氨基酸框架为在ohms1，而是没有结构的变化的第六exon的删除负责移动出现了。另外，即时荧光灯量的PCR分析证明OsMADS1表示水平在ohms1异种显著地减少了。发展相关的基因也显著地改变了的米饭flowering因素和花的颖的表示层次。这些结果证明OsMADS1可以在米饭起一个重要作用花的机关开发，特别地在花的颖开发和小花原基区别。

简介：在米饭的Phytochromes被由三个成员，PHYA，PHYB，和PHYC组成的一个基因家庭编码。通过以photomorphogenesis描绘phytochrome异种和野类型(WT)，单个phytochromes的角色preliminarily在调整米饭de青白化，flowering时间和富饶被探索了。然而，很少信息都没关于是否或怎么被报导phytochromes在米饭影响叶绿素生合成和叶绿体开发。在这研究，我们比较了野类型和phyA的叶绿素内容，在任何一个白人光(WL)下面成年或红的phyB和phyAphyB异种点亮(R)。结果建议phyB察觉R断然调整叶绿素生合成，当phyA的角色能仅仅在phyB缺乏的异种被检测时。涉及叶绿素生合成的基因的表示层次的分析表明phytochromes由调整protochlorophylloxidoreductaseA(PORA)影响了叶绿素生合成表示。在叶绿体开发的phyB的角色也被分析，并且结果建议phyB察觉R由影响叶绿体和grana的数字调整叶绿体开发，以及叶绿体膜系统。

简介：ThekeyforriceplantsurvivalunderNaClsaltstressismaintainingahighK+/Na+ratioinitscells.Selectionforsalttolerancericegenotypesbasedonphenotypicperformancealonewilldelayinprogressinbreeding.Useofmolecularmarkersintandemwithphysiologicalstudieswillhelpinbetteridentificationofsalttolerantriceaccessions.EightriceaccessionsalongwiththecheckDongjinwerescreenedusing1/2Yoshidasolutionwith50mmol/LNaClattheseedlingstage.TheaccessionsIT001158,IT246674,IT260533andIT291341wereclassifiedassalttolerantbasedontheirK+/Na+ratios.SeventeenSSRmarkersreportedtobeassociatedwithK+/Na+ratiowereusedtoscreentheaccessions.FiveSSRmarkers(RM8053,RM345,RM318,RM253andRM7075)coulddifferentiateaccessionsclassifiedbasedontheirK+/Na+ratios.BandingpatternoftheaccessionswasscoredcomparedtothebandingpatternofDongjin.ThestudydifferentiatedaccessionsbasedontheirassociationofK+/Na+ratiowithmolecularmarkerswhichareveryreliable.Thesemarkerscanplayasignificantroleinscreeninglargesetofricegermplasmsforsalttoleranceandalsohelpinidentificationofhigh-yieldingvarietieswithbettersalttolerance.Thesalttolerantaccessionscanbetakenforwardintodevelopingbettervarietiesbyconventionalbreedingandexploringgenesforsalttolerance.

简介：Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

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简介：Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.

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