简介：Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.

简介：TheeasternHimalayanregionofNortheast(NE)Indiaishometoalargenumberofindigenousricevarieties,whicharetraditionallyclassifiedasOryzasativasubspeciesindica,japonicaorintermediatetypes.TheclassificationbasedontraditionalCheng'sindexisofteninconclusiveduetophenotypicplasticityofmorphologicalcharacters,whichareinfluencedbyenvironmentalconditions.WeusedmolecularmarkersspecificforindicaandjaponicasubspeciestoassessthedegreeofgeneticrelatednessofindigenousricevarietiesinNEIndia.Theresultsrevealedthatmajorityofupland(jum)andglutinousricevarieties,traditionallyconsideredasjaponica,weregeneticallyclosetothesubspeciesindica.Allvarietiesofboroecotypewerefoundtobeindicatype,andonlyafewvarietiescultivatedinlowlandanduplandareaswerejaponicatype.Someofthelowlandvarietiesofthesaliecotypewereintermediatebetweenindicaandjaponica,andtheyshowedaclosergeneticaffinitytoO.rufipogon.

简介：thermo感觉迟钝的苍白的绿叶异种(pgl2)从T-DNA被孤立米饭的插入的转基因的线(OryzasativaL。subsp。装饰用的梨树cv。Nipponbare)。基因分析显示显型被一个后退的变化在单个原子编码基因引起。印射PGL2基因，一张F2人口被与Longtefu穿过异种构造(OryzasativaL。subsp。indica)。PGL2地点粗略地在染色体8上被连接到SSR标记RM331。细微地印射基因，14个新InDel标记在标记，和PGL2附近被开发进一步被印射到2.37Mbcentromeric区域。叶子的叶绿素内容上的分析证明在全部的叶绿素(Chl)异种和野类型之间没有明显的差别当在异种的Chla/Chlb的比率仅仅是大约1时，满足，它在野类型是比那显然低的，建议PGL2基因与在Chl之间的变换有关是andChlb。而且，在centromeric区域附近的教材设计的方法被讨论，它将提供卓见进在工厂着丝点的功能的基因的好印射。

简介：Genotypingbysequencing(GBS)istherecentapproachofnext-generationsequencingtechniquefordiscoveringandgenotypingsinglenucleotidepolymorphisms(SNPs)incropspecies.Genotypicvariationstudies(SNPsandinsertion-deletions/InDels)wereperformedusingfourricelinesbasedonGBSdatabyaligningtothereferencegenomeNipponbare.LocalaromaticricelandraceTulaipanjiwascrossedwithRanjit,andtwodistinctlineswereidentifiedfromtheprogenies:onelinewithawnsandaromatraitsandtheotherwithoutawnsandaroma.TotalnumberofSNPsandInDelsidentifiedwere52810and4327atreaddepth10,respectively.OutofthetotalpolymorphicSNPs/InDels,16490wereintergeneric,7812wereinsidegene,and4435wereintronic.Phylogenetically,Tulaipanjiwasclosertothereferencegenomenipponbare.Basedonrecurrentparentgenomeanalysis,outof10013alleles,92.52%wasintrogressedintoprogeny-awnfromTulaipanjiand7.48%fromRanjit,whereasprogeny-awnlesscarried89.19%allelesfromRanjitandonly10.81%allelesfromTulaipanji.Inaddition,progeny-awnwasthehighestheterozygous(83.88%)andprogeny-awnlesswastheleast(2.24%)atthisfifthgenerationofrecombinantinbredlines.TheseSNPvariationsmaybelinkedtothephenotypictraitsandcanbeutilizedincropimprovementthroughlinkagemapping.TheseresultssuggestthataddingahighdensityofSNPmarkerstoamappingorbreedingpopulationthroughGBShasagreatvaluefornumerousapplicationsinricebreedingandgeneticsresearch.