简介:Inducedembryonicstemresourcesfortheobservationofthecell(ES)cellsareexpectedtobepromisingcellbehaviorsindevelopmentalbiologyaswellastheimplantationincelltreatmentsinhumandiseases.ArecombinantE-cadherinsubstratumwasdevelopedasacellrecognizablesubstratumtomaintaintheEScells'self-renewalandpluripotencyatsinglecelllevel.Furthermore,thegenerationofvariouscelllineagesindifferentgermlayers,includinghepaticorneuralcells,wasachievedonthechimericproteinlayerpreciselyandeffectively.Theinductionandisolationofspecificcellpopulationwascarriedoutwiththeenhancingeffectofotherartificialextracellularmatrices(ECMs)inenzyme-freeprocess.ThemurineEScell-derivedcellsshowedhighlymorphologicalsimilaritiesandfunctionalexpressionstomaturedhepatocytesorneuralprogenitorcells.