简介：Arithmeticcodingisarelativelynewloss-lessdatacompressiontechniquethathasattractedmuchattentioninrecentyears.Weshowtheiterationofbit-levelarithmeticcodingcanbespecifiedbyacontinuousfunction.Theanalysisexpressionandsomepropertiesofthisfunctionarediscussed.Anapplicationofthefunctionisprovidedforexploringthesecurityofarithmeticcodeswhentheyareusedfordataencryption.

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简介：Fromthemolecularcurrentviewpoint,ananalyticexpressionexactlydescribingmagneticfielddistributionofrectangularpermanentmagnetsmagnetizedsufficientlyinonedirectionwasderivedfromtheBiot-Savart'slaw.Thisexpressionisusefulnotonlyforthecaseofonerectangularpermanentmagnetbulk,butalsoforthatofseveralrectangularpermanentmagnetbulks.Byusingthisexpression,therelationsbetweenmagneticfielddistributionandthesizeofrectangularpermanentmagnetsaswellasthemagnitudeofmagneticfieldandthedistancefromthepointinthespacetothetop(orbottom)surfaceofrectangularpermanentmagnetswerediscussedindetail.Allthecalculatingresultsareconsistentwithexperimentalones.Fortransversemagneticfieldwhichisamainmagneticfieldofrectangularpermanentmagnets,inordertodescribeitsdistribution,twoquantities,oneistheuniformityinmagnitudeandtheotheristheuniformityindistributionofmagneticfield,weredefined.Furthermore,therelationsbetweenthemandthegeometricsizeofthemagnetaswellasthedistancefromthesurfaceofpermanentmagnetswereinvestigatedbytheseformulas.Thenumericalresultsshowthatthegeometricsizeandthedistancehaveavisibleinfluenceontheuniformityinmagnitudeandtheuniformityindistributionofthemagneticfield.

简介：Bymeansofreasonableassumptionandmathematicalderivation,atheoreticexpressionofflowrateforasinglefracturewithlinearlyvaryingwidthwasobtained.Themathematicalderivationwasbasedonthecubiclawandthenewtheoreticexpressionwasanextentionoftraditionalparallelplatemodel.Thisstudymayhelptoanalyzeseepageinfracturedrockmass.

简介：Inthispaper,weshallgiveanAbeltypetheoremofJacobiseriesandthenbasedonitdiscussasymptoticexpressionsneartheellipseofconvergenceofJacobiseriesincomplexplane.

简介：Sumoylationisanimportantproteinmodificationdiscoveredrecently.SUMO(smallubiquitin-relatedmodifier)pathwayregulatestheproteinstabilityandtranscriptionalactivitywitha12-kDasmallmolecularprotein,SUMO,ligatedtothetargetprotein.ThepurificationofSUMOproteinsisakeysteptorevealtheirfunction.ThepurposeofthisstudywastoconstructtherecombinantSUMO1geneclonedtoapGEX-4T-1vectortoexpressandpurifytheSUMO1-GSTfusionproteininEscherichiacoli.First,thefulllengthDNAsequenceofSUMO1genewasamplifiedbyPCRandwasligatedtopMD18-Tvector.ThentheSUMO1genewassubclonedtopGEX-4T-1prokaryoticexpressionvectorbetweenBamHIandXhoIsites,andtransformedinEscherichiacoliDH5αcells.Therightcolonieswereidentifiedbyrestrictiveenzymedigestionandsequencing.ThecorrectrebombinantplasmidofpGEX-4T-1-SUMO1wastransformedinEscherichiacoliBL21cellsandtheninducedbyIPTG(isopropyl-β-D-1-thiogalacto-pyranoside)toexpresstheSUMO1-GSTfusionprotein.ThehighlypurifiedSUMO1-GST(glutathioneS-transferase)fusionproteinwasobtainedbyaffinitychromatography.Finally,thepropertiesofSUMO1-GSTfusionproteinwereconfirmedbyCoomassiebrilliantbluestrainandWesternblotanalysis.TherecombinantplasmidofpGEX-4T-1-SUMO1wassuccessfullyconstructed,andSUMO1-GSTfusionproteinsweresuccessfullyexpressed.

简介：TherootofPanaxginsengplantundergoesaspecificdevelopmentalprocesstobecomeabiosynthesisandaccumulationtissueforginsenosides.Toidentifyandanalyzegenesinvolvedinthebiosynthesisofginsenoside,weconstructedandcharacterizedafull-lengthcDNAlibraryfor6-year-oldNorthAmericanginseng.ThetiterofprimarycDNAlibraryis1.2×106pfu/mL,thetiterofamplifiedlibraryis2.6×1010pfu/mLandtherateofrecombinantisabove86%.Theinsertsizerangesfrom0.3to2.0kb.Sequencingresultsshowthat18of58genesarehighhomologoustothegenes(GBR5,GBR3andGBR1)knowninGenBank,whichareinvolvedinbiosynthesisofginsenosideinNorthAmericanginsengplant;16of58genesarenovelgenes.Thefull-lengthcDNAlibraryofNorthAmericanginsengroottissuesisessentialforthecloningofgenesknownanditisalsoaninitialkeyforthescreeningandcloningofnewgenes.

简介：Inthispaper,byconstructingtheone-to-onecorrespondencebetweenitsIFSandthequaternaryfractionalexpansion,then-thiterationanalyticalexpressionandthelimitrepresentationofthefamilyoftheKoch-typecurveswitharbitraryanglesareobtained.ThedistinctionbetweenourmethodandthatofH.SaganisthatweprovidethegenerationprocessanalyticallyandrepresentitasagraphofaseriesfunctionwhichlooksliketheWeierstrassfunction.Withthesearithmeticexpressions,wefurtheranalyzeandprovesomeofthefractalpropertiesoftheKoch-typecurvessuchastheself-similarity,theHo¨lderexponentandwiththepropertyofcontinuouseverywherebutdifferentiablenowhere.Then,wewillshowthattheKochtypecurvescanbeapproximatedbydifferentconstructedgenerators.BasedontheanalytictransformationoftheKoch-typecurves,wealsoconstructedmorecontinuousbutnowhere-differentiablecurvesrepresentedbyarithmeticexpressions.Thisresultimpliesthattheanalyticalexpressionofafractalhastheoreticalandpracticalsignificance.

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简介：ThispaperpresentsthelimitingexpressionforthegencalizedinverseAT.S(2)anditscorresgondingprojectorsSincecomononimnortorsinverses,suchasandAT.S(2)etcareallgeneralizedineeAT.S(2)Infact,wegiveaunifiedlimitingformulaofcomputinesuchimporiantgeneralinedinversesanditscorrespondingproiectors,BasedonthisweestalishandimbedlingmethodfirecompotingthegeneralizedinverseAT.S(2)Theresultsextendearlierworkbyvariousauthors.

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简介：TofindoptimalconditionsforexpressingthesolubleformofsFv-2F3andtostudythepurificationandpropertyofitsderivativeSe-sFv-2F3,thepreferredexpressionconditionswereinvestigatedbymeansoforthogonaldesign.Thesecultureconditionsincludedincubationtemperature,inducerconcentration,inductiontimeandcellconcentration.TheevaluationofexpressionwasaccomplishedbytheanalysisofwholecelllysatesandtheyieldofsolublesFv-2F3wascalculatedaccordingtotheanalysisofProfinder(FTI-500,Pharmacia).Thepurificationprocedurewascarriedoutviaatwo-steppurificationprocedureconsistingofion-exchangechromatography,followedbyimmobilizedmetalaffinitychromatography(IMAC).TheantioxidantefficacyofSe-sFv-2F3wasdemonstratedbythedeterminationofthecontentofthemainproductoflipidperoxidation,MDA,theviabilityofcellsandtheactivityofLDH.WeobtainedthepreferredcultureconditionstogrowtheengineeredbacteriaandtheprocedureforpreparingsolublesFv-2F3andconfirmedtheantioxidantefficacyofSe-sFv-2F3.

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简介：Leafcolormutantsareidealmaterialsforstudyingphysiologicalprocessesinplants.Here,athermos-sensitiveleaf-colormutantofGreenwanderingjewwasisolatedaftercarbonionsirradiation,whichwasdesignatedasmt.Thecolorofyoungmutantleaveswasmoresensitivetovariationsoftemperature,however,theyoungleavesofwildtyperemainedgreenunderlow-temperatureconditions(6℃-20℃).Toelucidatethecharacteristicsofpigmentationinmutantleavesunderroomtemperatureconditions(25℃)andlow-temperatureconditions(7℃),theultra-structural,pigmentcomposition,molecularmechanismsandanthocyaninaccumulationinvolvedinthisphenomenonhavebeeninvestigatedinfourindependentexperiments.