简介:TheaimofthisstudyistoexplorethegenomicmolecularorganizationandgenogroupofhumannorovirusfrominfectedinfantsinGuangzhouofChina.PrimersweredesignedaccordingtothegenomicsequenceofnorovirusintheGenBank,andthenorovirusgenomewasamplifiedbyRT-PCR.ThePCR-productswereclonedintoTvectorandsequenced,andthegenomicnucleotidesequenceswereanalyzedwiththeprogramsCLUSTALW/X,DNASTARandRAT(RecombinationAnalysisTool).TheNVgz01straingenomeis7558bpinlengthandencodesthreeopenreadingframes(GenBankaccessionNo.isDQ369797).ThegenomicsequencesofNVgz01werecomparedwiththoseofnomvirusinGenBank,whichrevealedthatthehomologywithgenogroupⅡrangesbetween76%-90%,andgenogroupⅠbe-tween43%-44%.TheORF1regionshared94%and88%identitywithMc37andFarmingtonstrains,respectively;thecapsidregion(ORF2)shared65%and94%identitywithMc37andFarmingtonstrains,respectively.Phylogenetictreeswerereconstructedbytheneighbor-joiningmethod.ComparativecompletesequenceanalysisoftheNVgz01withreportedhumannorovimsgenomicsequencesrevealedthatthisisolatebelongstogenogmupⅡ.TheORF1andORF2regionsshareddifferentidentitywithMc37andFarmingtonstrains,suggestingNVgz01couldbearecombinantvires.
简介:摘要在法医医学鉴定过程中经常会遇到基因丢失的情况,导致亲子代在基因鉴定过程中出现不符合遗传规律的情况,在一定程度上增加了被错误排除的风险。由于等位基因的缺失在一定程度上和模板的DNA多态性、碱基的插入或缺失有关,因此正确识别和分析等位基因对鉴定结果会产生直接的影响。基于此,本文作者结合自身实践就TH01基因座丢失的相关问题进行相关阐述。
简介:ToanalyzethegenomicmolecularstructureandgenotypeofhumanastrovirusisolatedfrominfantinGuangzhouofChina,theprimersweredesignedbasedonthegenomicsequenceofastrovirusfromtheGenBankandthetargetsequencewereamplifiedbyRT-PCR.ThenthePCR-productswereclonedtoTvectorandsequenced.ThegenomicnucleotidesequenceswereanalyzedbytheprogramsCLUSTALWandDNASTAR.ItwasfoundthatthefullgenomiclengthofHASTVgz01strainwas6721bpandtheORFswere6558bp.The5'and3'UTRwere82and81nucleotides.Thegenomeincluded3openreadingframes(ORFs):ORF1a,ORF1bandORF2.The5'-terminalORF1astartedatnucleotide83andextendedtonucleotide2845.ORFlb(nt2785tont4332)overlapedORFlaby61nucleotides.The3'-terminalORF2beganatnucleotide4325andterminatedatnucleotide6640.ORF2had2316nucleotides.ComparedwithotherastrovirussequencesinGenBank,thehomologyoftheaminoacidsequenceofORF2ofHASTVgz01strainwiththatofserotype4was93%.Homologywithotherserotypesrangedfrom61%to70%.ThecompletenucleotidesequenceofastrovirusHASTVgz01strainisolatedfromGuangzhouinChinawas6721bpinlength,GenBankaccessionNO.DQ344027.ComparingtheORF2ofastrovirusHASTVgz01withtheknownsequencesoftypes1-8thehighesthomologywasserotype4(93%).ComparativesequenceanalysisoftheHASTVgz01ORF2withthereportedhumanastrovirussequencesrevealedthattheisolatedastrovirusbelongstogenotype(serotype)4.
简介:一元复始,万象更新,值此新年到来之际,我谨代表世界中医药学会联合会(以下简称“世界中联),向世界中联全体会员致以诚挚的问候,向关心和支持世界中联事业发展的世界各国中医药团体致以崇高的敬意和新年的祝福!向一直关注和支持中医药事业发展的各界领导和专家、学者、同仁们表示衷心地感谢!祝大家在新的一年里生活幸福,工作顺利,平安吉祥!
简介:Livertransplantation(LT)isalife-savingtreatmentforpatientswithend-stageliverdiseaseandforpatientswithlivercellcancerrelatedtoliverdisease.Acuteandchronicliverdiseasesrelatedtohepatitisvirusesarebetweenthemainindicationsforlivertransplantation.Theriskofviralreinfectionaftertransplantationisthemainlimitingfactorintheseindications.Beforetheavailabilityofantiviralprophylaxis,hepatitisBvirus(HBV)recurrencewasuniversalinpatientswhowereHBVDNA-positivebeforetransplantation.ThenaturalhistoryofrecurrentHBVwasacceleratedbyimmunosuppression,anditprogressedrapidlytograftfailureanddeath.Introductionofpost-transplantprophylaxiswithimmunoglobulinalonefirst,andassociatedtoantiviraldrugslater,drasticallyreducedHBVrecurrence,resultinginexcellentlong-termoutcomes.Onthecontrary,recurrenceofhepatitisCisthemaincauseofgraftlossinmosttransplantprograms.Overall,patientandgraftsurvivalafterLTforhepatitisCvirus(HCV)-associatedcirrhosisisinferiorcomparedwithotherindications.However,successfulpretransplantorposttransplantantiviraltherapyhasbeenassociatedwithincreasedgraftandoverallsurvival.Untilrecently,thecombinationofpegylatedinterferonandribavirinwasthestandardofcareforthetreatmentofpatientswithchronichepatitisC.Highlyactiveantiviralcompoundshavebeendevelopedoverthepastdecade,thankstonewinvitrosystemstostudyHCVentry,replication,assembly,andrelease.
简介:摘要目的探讨临床肩难产的处理方法及母婴并发症。方法对我科2008年1月~2011年12月经阴道分娩的1386例产妇当中的11例肩难产患者的临床资料进行回顾性分析,并就其肩难产的处理方法及母婴并发症予以总结。结果11例肩难产患者通过屈大腿法、耻骨联合压前肩法、旋肩法、娩后肩法均成功分娩。发生新生儿窒息4例,锁骨不完全性骨折1例,新生儿臂丛神经损伤1例,新生儿颅内出血1例,产妇会阴Ⅲ°裂伤2例,产后出血1例,后果比较严重。结论肩难产是产科的较少见的并发症,但处理不当,会造成母婴严重后果,助产者不但要熟练掌握处理肩难产的方法,还要预测及预防肩难产的发生,降低母婴并发症。
简介:有人说,从事过美容化妆品行业策划的人去任何一个行业从事策划都没有问题。笔者从事美容行业十余年来,从个人角度观察这个行业,始终觉得作为一个成熟的品牌、一个成功的企业要想做好企业整体市场运营、品牌策划及推广,会议营销是不可或缺的重要环节。招商会、答谢会、终端会议、培训会及各类庆典,已经成为企业开拓市场、扩大影响力、增进收益的重要营销模式。笔者曾成功参与策划、组织、实施会议活动达300多场,涉及到美容招商会、答谢会、促销会、联谊会等室内外各类型会议,深刻感受到细节化操控、专业高效化执行、流程化系统运作才是成功运作会议的关键。总结长久以来的会议实操经验,笔者整理出“成功运作会议11字”,与大家分享。