简介：AbstractDrug resistance via drug-resistant mutations in the human immunodeficiency virus (HIV) genome is the primary cause of antiviral therapy failure. Consequently, HIV drug resistance genotyping has become a critical approach in HIV prevention and control. Compared to the Sanger sequencing technology, high-throughput sequencing (HTS) technology has superior sensitivity and timeliness, with strong detection capabilities for low-frequency mutations. With the continued advancement of HTS technologies, their prominence in HIV drug resistance detection techniques has increased accordingly. This article will review the latest developments in HTS technology and its applications in HIV drug resistance testing.

简介：Objective:Hepatocellularcarcinoma(HCC)isaleadingcauseofcancer-relateddeaths.NovelserumbiomarkersarerequiredtoincreasethesensitivityandspecificityofserumscreeningforearlyHCCdiagnosis.ThisstudyemployedaquantitativeproteomicstrategytoanalyzethedifferentialexpressionofserumglycoproteinsbetweenHCCandnormalcontrolserumsamples.Methods:Lectinaffinitychromatography(LAC)wasusedtoenrichglycoproteinsfromtheserumsamples.Quantitativemassspectrometricanalysiscombinedwithstableisotopedimethyllabelingand2Dliquidchromatography(LC)separationswereperformedtoexaminethedifferentiallevelsofthedetectedproteinsbetweenHCCandcontrolserumsamples.Westernblotwasusedtoanalyzethedifferentialexpressionlevelsofthethreeserumproteins.Results:Atotalof2,280proteingroupswereidentifiedintheserumsamplesfromHCCpatientsbyusingthe2DLC-MS/MSmethod.Upto36proteinswereup-regulatedintheHCCserum,whereas19proteinsweredown-regulated.Threedifferentialglycoproteins,namely,fibrinogengammachain(FGG),FOS-likeantigen2(FOSL2),andα-1,6-mannosylglycoprotein6-β-N-acetylglucosaminyltransferaseB(MGAT5B)werevalidatedbyWesternblot.Allthesethreeproteinswereup-regulatedintheHCCserumsamples.Conclusion:AquantitativeglycoproteomicmethodwasestablishedandprovenusefultodeterminepotentialnovelbiomarkersforHCC.

简介：Hearingloss(HL)isthemostcommonsensorydisorder,affectingallagegroups,ethnicities,andgen-ders.AccordingtoWorldHealthOrganization(WHO)estimatesin2005,278millionpeopleworldwidehavemoderatetoprofoundHLinbothears.Resultsofthe2002NationalHealthInterviewSurveyindicatethatnearly31millionofallnon-institutionalizedadults(aged18andover)intheUnitedStateshavetroublehearing.Epidemiologicalstudieshaveestimatedthatapproximately50%ofprofoundHLcanbeattributedtogeneticcauses.Withover60genesimplicatedinnonsyndromichearingloss,itisalsoanextremelyhet-erogeneoustrait.Recentprogressinidentifyinggenesresponsibleforhearinglossenablesotolaryngologistsandotherclinicianstoapplymoleculardiagnosisbygenetictesting.Theadventofthe$1000genomehasthepotentialtorevolutionizetheidentificationofgenesandtheirmutationsunderlyinggeneticdisorders.ThisisespeciallytrueforextremelyheterogeneousMendelianconditionssuchasdeafness,wherethemuta-tion,andindeedthegene,maybeprivate.Therecenttechnologicaladvancesintarget-enrichmentmethodsandnextgenerationsequencingofferauniqueopportunitytobreakthroughthebarriersoflimitationsim-posedbygenearrays.Theseapproachesnowallowforthecompleteanalysisofallknowndeafness-causinggenesandwillresultinanewwaveofdiscoveriesoftheremaininggenesforMendeliandisorders.Thisre-viewfocusesondescribinggenotype-phenotypecorrelationsofthemostfrequentgenesincludingGJB2,whichisresponsibleformorethanhalfofcases,followedbyothercommongenesandondiscussingtheim-pactofgenomicadvancesforcomprehensivegenetictestingandgenediscoveryinhereditaryhearingloss.

简介：AbstractBackground:Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species levels. For large scale screening, such as malaria screening for elimination, diagnostic assay can be a challenge, as both the throughput and cost of the assay must be considered. The requirement of nucleic acid extraction hampers the throughput of most molecular assays. Co-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening. A genus-and species-specific diagnostic assay with simplified procedure, high sensitivity and throughput is still needed. This study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease screening.Methods:We developed multi-section Capture and Ligation Probe PCR (mCLIP-PCR) for the direct detection of RNA without extraction and reverse transcription. Multiple tailed sandwich hybridization probes were used to bind at genus-and species-specific sections of the target RNA to cooperatively capture the target onto a 96-well plate. After enzymatic ligation of the bound probes, a single-stranded DNA formed at each section with distinct tail sequence at the ends. They were separately PCR-amplified with primers corresponding to tail sequences for genus or species identification. We applied the method to the active screening of Plasmodium infections of 4,580 asymptomatic dried blood spot samples collected in malaria endemic areas and compared the results with standard qPCR using linear regression.Results:With multi-section cooperative capture but separate amplification strategy, we accurately identified genus Plasmodium and species P. falciparum and P. vivax without RNA extraction, with favorable sensitivities among the published reports. In the active screening, our method identified all 53 positive infections including two mixed infections, and two P. vivax infections that were missed by standard qPCR.Conclusions:mCLIP-PCR provides a sensitive and high-throughput approach to large-scale infectious disease screening with low cost and labor, making it a valuable tool for malaria elimination in endemic region.

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简介：Noise-inducedhearinglossisacommoncauseofacquiredhearinglossintheadultpopulation.Acousticoverstimulationcausescochleardamagethroughmechanicalstresstothetissue.Consequently,complexmolec-ularchangesareinitiated,andthesechangesleadtomorphologicalandbiologicalalterationsinthecochlea,whichinturncompromisethecochlearfunctionandcausehearingloss.Inthepast10years,therehavebeensignificantadvancesinourunderstandingofthemolecularmechanismsofnoise-inducedhearingloss.Theseadvancesareattributed,inpart,tothedevelopmentofhigh-throughputtechnologiesfortheglobalanalysesofmolecularchanges.Inthisreview,webrieflydescribethenewlydevelopedmethodsforinvestigatingthemo-lecularresponsesofthecochleatoacoustictraumaandtheknowledgegeneratedfromthesestudies.Wealsodiscussthestrengthsandlimitationsofeachtechniqueandthemajorchallengestoinvestigatecochleardegen-erationfollowingacousticinjury.

简介：Thispaperpresentsaflexibleandhighspeeddigitalscanconverter(DSC)withtheabilitytohandlehighfrequencyultrasoundimaginginreal-time.Thecharacteristicsinimagingsystemsuchasfocuslengthoftransducer,theswingradiusandsamplinglengthetc.couldbechangedeasilyincompliancewiththeresearcher'sapplicationbasedonthisflexibledigitalscanconverter.Linearinterpolationisemployedtoachievethecoordinatetransformationsalgorithm.Custom-builtsoftwareisprogrammedtopreliminarilyhandlethealgorithmaccordingtodifferentultrasoundimagingapplications.HighperformanceFPGAwillimplementhighspeedinterpolationcalculationbasedonthepreliminarydatawhicharestoredintheDDR2SDRAMfromthesoftware.64bit66MHzPCIisemployedtoaccomplishhighspeeddatatransmission.Experimenthasshownthatmorethan500frameratecouldbeachievedbasedonthishighspeeddigitalscanconverter.ThedesignedflexibleandhighspeeddigitalscanconvertercouldbeusedincurrentFPGAbasedhighfrequencyultrasoundimagingsystem.

简介：High-frequencyimagetechniquehasbeenwidelyappliedinmedicaldiagnosisrecently.Forhighvoltageprotection,highspeedstageandtriggercontrolcircuitryaredifficulttoimplementahigh-frequencyultrasoundimagingsystem.Inthisstudy,weutilizedalinearservowithhighnoisetoleranceandanovelmulti-depthexpressionmethodtoovercomethoseissuesindevelopedhigh-speedimagesystem.B-modeimageofthechickenphantomby25MHztransducershowstheresolutionoflateralandaxialresolutionsareupto123μmand59μmrespectively.Inaddition,theexperimentdemonstratesthattheaxialresolutionanddepthoffield(DOF)canbeimprovedbytimegaincompensation(TGC)andmulti-depthmethod.Theresultsindicatethattheproposedsystemcouldachieveover24fpsfor1mmscandistanceand100linesperframe.Inthefuture,thedevelopedsystemispotentialforotherclinicalapplicationssuchasophthalmologyanddermatology.

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简介：Ananomeshhasbeendevelopedthatnearlytriplessolarcellefficiency.Theresearchteammanagedtoreducethereflectivityofthematerialsused,effectivelyincreasingtheamountofcapturedlight.Inadditiontothis,thenewsolarcellsarealsomuchthinnerthancurrentonesonthemarket.Thestructureiscreatedbysandwichingmetalwithnanomeshandisbeingcalled'PlasomnicCavity

简介：Poly(methylmethacrylate)(PMMA),usedasdenturebasematerialsinden-tistry,hassomeinherentdefects,suchaslowertoughnessorhigherbrittlenesswhichisoneofthemainreasonsofthefractureofdenturebaseinclinical.Therefore,manyscientistshavefocusedonhighimpactstrengthdenturebasematerials.Inthispa-per,usingMethylmethacrylate-butadiene-styrene(MBS)asareinforcementagent,

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简介：目的：提高高职类体育院校推拿手法学的教学质量。方法：进行了多项教学改革，包括按认知规律重新安排教学内容，引入PBL教学理念，改善教学条件，增加了多媒体及实践教学，丰富了教学资源，改革了考核评价方案等。结果与结论：通过各项改革，学生不仅学到了专业技能，还提高了分析、解决问题的能力。

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简介：<正>DearSir,Oculartraumaisatopicofunresolvedcontroversiesandtherearecontinuouscontroversialanddebatablediagnosticandmanagementstrategiesforopen-globeinjuries[1].Amongstmanytypesofoculartrauma,theopenglobeinjuryisthemostseriousduetoitsverypoorvisualprognosisandyoungpopulationofpatientsaremostlyaffected[2].Thetreatmentoutcomemaybeimprovedbypromptdiagnosis,andimmediatesurgicalrepairperformedtohighstandard[3].Amongsttheotherclinicalsignstheintraocularpressure(IOP)isfoundtobeparticularlyreducedandconventionallythisisconsideredasaveryreliableindicatorofoccultglobe

简介：Objective:Toevaluatethesafetyandefficacyofexternalhigh-intensityfocusedultrasound(HIFU)castrationforbreastcancerouspatientsaftermastectomyiftheyareatahighriskofrecurrence.Methods:Werecruited52consecutivepatientswithprimaryoperablebreastcancerwhoweretreatedwithmastectomywithexcisionofregionallymphnodes.PatientswerepositiveforERandPRimmuno-cytochemicalstaining,node-positive,un-menopause,over40yearsoldandweredividedintotwogroupsrandomly.Forcastration,26patientsreceivedoneortwotimesofHIFUtreatmentwithinfivedays,andtheotherpatientsreceivedradiotherapywithDT18Gy/9f/11days.Duringandafterthetreatment,localchangesandsystemicresponseofthepatientswereobserved.Results:After1monthtreatment,levelsofserumE1andE2weresignificantlydecreasedcomparedtobeforetreatmentinHIFUgroups(P<0.01andP<0.001).Thesamechangeswereoccurredinradiotherapy(RT)groups(P<0.05andP<0.01).ThelevelsofserumE1orE2inRTgroupswerehigherthaninHIFUgroups(P<0.05).Thesymptomdistributionof'climactericsyndrome'ofHIFUgroupsweresignificantlydifferentfromRTgroups(P<0.01).Thefollow-uptimewas4months.Theincidenceofamenorrheawas100%inallpatients.Noseriouscomplicationswereseen.Thetemperature,pulse,bloodpressure,andrespiratoryrateofthepatientswerealmostnormal.Conclusion:WehaveshownthattheuseofHIFUinthecastratingofpatientswithbreastcancerisfeasible,safeandeffective.ThistechnologymayprovidearapidnoninvasivealterativetoconventionalbilateraloophorectomyorRTcastration.

简介：客观：借助于高分辨率图象分析(HRIA)预言食道的上皮的发育异常的结果。方法：无征状的成年人从Linxian县的Heshun公社在1983为食管的汽球细胞学被检查。93严重发育异常和食管的122温和发育异常被选择。借助于与电视照相机装备的一台Axiomat显微镜，在染色奶头的有鳞的上皮的中间的层的保存得很好的房间的100个正常原子核随机被检验。结果：93个cytologically诊断的严重发育异常盒子，24,14和7分别地在3，5和9年里进行了到癌。在另外的48个盒子中，发育异常仍然是马厩或regressed到正常。另外的盒子被用作控制。根据染色质特征，盒子的正确诊断被HRIA在75.0%完成(18/24)，85.7%(12/14)并且(6/7)85.7%盒子检验了，分别地(P<0.001)。122cytologically诊断的温和发育异常，16,13和12个盒子分别地在3，5和9年里进行了到癌。另外的81个盒子仍然是马厩或regressed到正常。正确诊断被HRIA在93.8%做(15/16)，76.9%(10/13)并且(10/12)833%盒子检验了，分别地(P<0.001)。结论：HRIA检验的染色质原子特征能预言癌症前期的损害的结果并且从non-progressor区别progressor。它能为chemo预防试用的效率的评估被用作代理人端点biomarkers。

简介：Basedontheinvestigationofthefourdifferentcompositiondentalamalgamal-loysmadebysametechnologricalprocess,therelationbetweenphysicalpropertiesandmicrostructureofthemhasbeendiscussedindetail.Thetheoryfoundationhasbeenexaminedforthedesignofdentalamalgamalloy.Themainconclusionsareasfollows:

简介：AbstractBiosafety equipment is the key barrier enabling high containment laboratories to handle high risk agents that may cause serious and potentially lethal infections. This perspective thoroughly analyzes the development of many kinds of key biosafety technologies, and equipment for protection of laboratory workers and for high containment laboratory facilities in China. Over more than ten years of rapid development, China has had remarkable achievements in key biosafety technologies and equipment in high containment laboratories. These technologies basically meet the needs of high containment laboratories construction in China. Furthermore, according to the current global situation regarding the prevention and control of infectious diseases and the technical development level of biosafety equipment, this paper proposes targeted suggestions and notes that China still needs to increase investment in scientific research to provide further technical and equipment support enabling us to build a community with a bright future in terms of human biosafety.

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