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简介：Twonewfuranfragmentisomerizedlimonoids,meliazedalidesAandB(compounds1and2),wereisolatedfromthefruitsofMeliaazedarachLinn..TheirchemicalstructureswereelucidatedonthebasisofHR-ESI-MSand1Dand2DNMRdata,whichbelongedtonimbolinin-andtrichilin-class,respectively.Compound2exhibitedweakinhibitoryeffectonNOproductioninlipopolysaccharide(LPS)-activatedRAW264.7macrophageswithIC50being37.41μmol·L^-1.

简介：TherootbarkofMorusalbaL.orwhitemulberryiswidelyusedastraditionalmedicineinChina,JapanandKorea.Majorclassesandtypesofphenoliccompoundsisolatedfromtherootbarkareflavonoids(kuwanons,morusin,cyclomorusinandsanggenons),benzofurans(moracinsandmulberrofurans),andstilbenoids(mulberrosides).SomeoftheflavonoidsandbenzofuransareproductsofDiel-Aldertypeadducts.Otherclassesofcompoundsincludetriterpenes,phenolicacidsandcoumarins.Morusin,aprenylatedflavonoid,wasfirstisolatedfromtherootbarkofM.alba,andlaterfromtheleaf,stembarkandtwigoftheplant.Thepotentanti-cancerpropertiesofmorusinhaveattractedmuchattentionwithresearchon-goingandnewfindingsbeingpublished.Thecompoundinhibitsangiogenesis,tumourprogressionandtumourmigration,andtriggersapoptosis,cellcyclearrestandautophagyincolorectal,cervical,prostate,breast,hepatoma,pancreatic,glioblastoma,gastric,ovarianandlungcancercelllines.Theanti-canceractivitiesofmorusinareexecutedviavariousmoleculartargetsandsignallingpathways.Itisanticipatedthaton-goinginvitrostudieswillprogressgraduallytoinvivostudiesusinganimalmodelsbeforeeffortstowardsdrugdevelopmentcanbeinitiatedforclinicaltrials.

简介：摘要目的分析祁阳县2012－2017年麻疹流行病学特征，探讨麻疹消除策略。方法采用描述流行病学方法对祁阳县2012－2017年实验室确诊麻疹病例进行描述性流行病学分析。结果祁阳县2012－2017年麻疹发病率在0.09－8.22/10万之间，呈较低的发病水平。发病年龄以8月龄以下儿童最多42.15%。发病儿童麻疹免疫史较低，8月龄—18月龄儿童1次免史25%，18月龄—3岁儿童1次免疫史42.85%、2次免疫28.57%，3岁—7岁儿童2次免疫史20%，1次免疫史30%，7岁以上的人群2次免疫史40%。结论提高麻疹类疫苗接种是控制麻疹发病的关键。

简介：Objective:MemorystemTcells(Tscm)haveattractedattentionbecauseoftheirenhancedself-renewal,multipotentcapacity,andanti-tumorcapacities.However,littleisknownaboutTscminpatientswithrenalclearcellcarcinoma(RCC)andtheroleofWntsignalinginthesecells.WeevaluatedTscmfromRCCpatientsconcerningtheiractivationofWntsignalinginvitroandexploredthemechanismofpreferentialsurvival.Methods:FlowcytometryidentifiedsurfacemarkersandcytokinesproducedfromaccumulatedTscminthepresenceoftheglycogensynthasekinasebetainhibitorTWS119.Apoptosiswasevaluatedafterinductionusingtumornecrosisfactor-alpha.ImmunofluorescenceandWesternblotanalyseswereusedtoinvestigatetheactivationofthenuclearfactor-kappaB(NF-КB)pathway.Results:RCCpatientshadasimilarpercentageofCD4~+andCD8~+Tscmashealthydonors.ActivationofWntsignalingbyTWS119resultedintheaccumulationofTscminactivatedTcells,butreversalofdifferentiatedTcellstoTscmwasnotachieved.PreferentialsurvivalofTscmwasassociatedwithincreasedanti-apoptoticabilitymediateddownstreamoftheNF-КBactivationpathway.Conclusions:ThefindingthatTscmcanaccumulatebyWntsignalinginvitroinbloodfromRCCpatientswillhelpindevisingnewcancertherapystrategiesofTscm-basedadoptiveimmunotherapy,suchasdendriticcell-stimulatedTscm,andTcellreceptororchimericantigenreceptor-engineeredTscm.

简介：摘要目的分析南充市2012-2016手足口病原学特征，为制定针对性预防措施提供依据。方法收集该市2012-2016手足口病原学监测资料，采用描述流行病学方法进行分析。结果共检测1650例，肠道病毒通用型核酸阳性率67.4%(1112/1650)，其中CVA16组病毒构成比28.1%（312/1112），肠道EV71构成比38.3%（426/1112)，EV未分型构成比33.6%(374/1112)，以肠道EV71型的为主。监测病例数男女比例为1.421，阳性病例数男女比例是1.501，男性阳性率68.8%高于女性阳性率63.3%；0～3岁组的阳性率为65.2%（805/1234）。结论南充市2012-2016手足口病优势病原体为肠道EV71和未分型肠道病毒，以6岁以下患儿为主，男女生监测病例病毒阳性检出率无显著差异。

简介：AIM:Toconstructfunctionalhumanfull-thicknesscornealreplacements.METHODS:Acellularporcinecornealmatrix(APCM)wasdevelopedfromporcinecorneabydecellulariztion.Thebiomechanicalpropertiesofanterior-APCM(AAPCM)andposterior-APCM(PAPCM)werecheckedusinguniaxialtensiletesting.Humancornealcellswereobtainedbycellculture.Suspendingringwasdesignedbydeformationofanacupunctureneedle.MTTcytotoxicityassaywasusedtocheckthecytotoxicityofsuspendingringsoakingsolutions.Anewthree-dimensionalorganculturesystemwasestablishedbycombinationofsuspendingring,48-wellplateandmediumtogether.Ahumanfull-thicknesscornealsubstitutewasconstructedfromhumancornealcellswithAAPCMinanorgancoculturesystem.Biochemicalmarkerexpressionoftheconstructwasmeasuredbyimmunofluorescentstainingandmorphologicalstructureswereobservedusingscanningelectronmicroscopy.Pumpfunctionandbiophysicalpropertieswereexaminedbypenetratingkeratoplastyandfollow-upclinicalobservations.RESULTS:TherewerenocellsintheAAPCMorPAPCM,whereascollagenfibers,Bowman’smembrane,andDescemet’smembranewereretained.ThebiomechanicalpropertyofAAPCMwasbetterthanPAPCM.HumancornealcellsgrewbetterontheAAPCMthanonthePAPCM.Therewasnocytotoxicityforthesuspendingringsoakingsolutions.Fortheconstructedfull-depthhumancornealreplacementskeratocytesscattereduniformlythroughouttheAAPCMandexpressedvimentin.TheepitheliallayerwaslocatedonthesurfaceofBowman’smembraneandcomposedofthreeorfourlayersofepithelialcellsexpressingcytokeratin3.OnelayerofendothelialcellscoveredthestromalsurfaceofAAPCM,expressedNa+/K+ATPaseandformedtheendotheliallayer.Theconstructwassimilartonormalhumancorneas,withmanymicrovilliontheepithelialcellsurface,stromalcellswithalongshuttleshape,andzonulaoccludensontheinterfaceofendothelialcells.Theconstructwithstoodsurgicalprocedu

简介：目的了解广东省梅州市2012-2017年腹泻患者中诺如病毒(norovirus,NV)和轮状病毒(rotavirus,RV)的感染情况和流行趋势。方法采集梅州市监测门诊中'腹泻病例'的粪便标本和/或呕吐物标本3066份,采用实时荧光定量PCR法开展NV(GI、GII)核酸检测与RV抗原检测。结果检出NV和RV阳性823份,总阳性率为26.84%,其中NV阳性率为13.24%,RV阳性率为13.60%;2012-2017年连续检测显示NV阳性率呈波动趋势,RV阳性率呈逐年下降趋势;NV和RV感染具有季节性,高峰期分别在10-11月和1-2月。不同年龄组之间差异有统计学意义,且5岁及以下年龄组为主要感染对象。结论梅州市病毒性腹泻病原NV和RV在全年各月份均有检出,以冬春季为感染高峰。阳性率总体有所下降但仍需引起关注,继续加强监测,以减少疾病的流行。

简介：摘要目的分析溧阳市2012年—2016年结核病流行病学特征，掌握影响结核病发病的因素，为结核病防控工作提供科学依据。方法对溧阳市2012年—2016年结核病疫情资料进行描述性流行病学分析。结果溧阳市2012年—2016年累计报告肺结核1506例，5年肺结核报告平均发病率38.18/10万，各年度发病率差异有统计学意义(χ2=9.53，P＜0.05)。男女发病相对比为3.251，男性和女性肺结核患者5年平均发病率分别为57.59/10万和18.09/10万。发病例数最多的是45岁～年龄组，占15.67%，其次是55岁～年龄组和15岁～年龄组，分别占15.27%和14.81%。职业以农民为主，占55.51%。7～9月份的秋季为发病高峰，发病人数占总发病人数的29.28%，3～4月份为发病小高峰，发病人数占总发病人数的18.73%。5年登记平均肺结核发病率较高的为社渚和溧城，常州监狱、溧阳监狱中5年登记平均肺结核发病率亦较高。结论近年来溧阳市肺结核防控工作得到重视，防治措施落实有效。溧阳地区青壮年外出就业者居多有关，社会活动频繁，暴露机会较大。青少年内分泌系统变化大、学习压力大、身体锻炼少、对营养要求高，增加了感染风险。应关注本市外出就业人群及初高中学生的肺结核防控工作。监狱内肺结核高发，应高度重视监狱等人群高度聚集环境中的肺结核防控工作。