简介:根据转录组数据库,通过同源克隆获得结缕草ZjSPL4基因,开放阅读框为1002bp,编码333个氨基酸,该基因具有SBP基因家族保守域。ZjSPL4编码的蛋白分子量为36.38163kD,理论等电点(pI)为8.79,总平均亲水性为-0.707,属于亲水性蛋白。二级结构预测结果显示ZjSPL4蛋白主要由无规则卷曲(Cc)、α螺旋(Hh)构成。ZjSPL4蛋白与其他植物的SBP蛋白总体相似度为53.71%,通过系统进化树分析发现ZjSPL4与谷子和二穗短柄草中的SPL基因亲缘关系较近。ZjSPL4蛋白亚细胞定位结果显示,其编码的蛋白定位于细胞核中。同时日本结缕草ZjSPL4基因受到GA、ABA、蔗糖、低温的诱导,说明该基因可能参与结缕草花芽分化以及抗逆反应等过程。因此,克隆分析结缕草ZjSPL4基因可为结缕草花芽分化与抗性机制研究以及分子育种提供一定参考。
简介:Inthisarticle,weinvestigatedeffectsofleadandcadmiumonthebiomassofginkgoseedlings,androlesoftheseedlingsinaccumulationcharacteristicsoftheheavymetalsandremediationofthesoilcontaminatedbyCd2+orPb2+aswellastheircombination.TheresultsshowedthatbothCd2+andPb2+significantlyorverysignificantlyreducedbiomassincrementofginkgoseedlings,withPb2+moreadversethanCd2+.TheCd2+-Pb2+compoundpollutionfurtherreducedbiomassincrementofginkgoseedlings.ThemassfractionsandenrichmentcoefficientofPb2+andCd2+inginkgoseedlingswereinanorderofroot>stem>leaf,whichwasinfavoroftransportationPb2+andCd2+fromroottostemandleave.TheCd2+-Pb2+compoundpollutionpromotedginkgoseedlingabsorptiontoCd2+andPb2+.ThecapacityoftheupwardtransportofPb2+wasgreaterthanthatofCd2+,whichsuggestedanobviousdose-effectandsynergisticeffect.Cd2+andPb2+accumulationsinginkgoseedlingswererelativelylarge.Theaccumulationinrootswashigherthanthatinstemsandleaves.Theabilityoftheabsorption,transportationandaccumulationofginkgoseedlingstoPb2+wasrelativelygreaterunderCd2+-Pb2+compoundpollution.ThephytoremediationefficiencyofCd2+andPb2+wasrelativelyhigh.
简介:Thetitlecompounds4,4'-oxobis(propham)((?)n)(C20H24N2O5,Mr.=372.41)wassynthesizedbytheadditionreactionofcorresponding4,4'-oxobisphenyldiisocyanatewithisopropanol.ThecrystalstructurewasdeterminedbyX-raydiffractionmethod.Thecrystalistriclinicsystem,spacegroupP-1witha=8.5107(17),b=9.1164(18),c=14.5701(3)A,α=80.44(3),β=85.25(3),γ=62.88(3)°,V=992.2(3)A3,Z=2,Dc=1.247g/cm3,F(000)=396,R=0.0520,wR2=0.1434for4336observedreflectionswith[I>2σ(I)].X-rayana...
简介:Cd忍耐和米饭幼苗的translocation上的H2O2预告的处理的效果用在Cd忍耐不同的二米饭栽培变种(N07-6和N07-63)被学习。malondialdehyde(MDA)的内容,减少的谷胱甘肽(GSH),非蛋白质thiols(NPT),phytochelatins(PC)和谷胱甘肽S-transferase(GST)的活动在暴露于各种各样的处理的二栽培变种之间被比较。结果证明50mol/LCd暴露显著地禁止了米饭生长,提高了GSH,NPT,PC和MDA的生产,并且增加了GST的活动,并且二栽培变种之间有重要差别。更多的Cd被搬运进N07-6的射击。H2O2预告的处理由进一步在根增加GSH,NPT和PC内容,以及GST活动减轻了Cd毒性。在N07-63的这些参数的增加度比在N07-6的那些高,建议N07-63的忍耐比N07-6更显著地被提高。氢过氧化物把Cdtranslocation归结为米饭射击,但是不同地在根影响了Cd内容。从上述结果,在到在二栽培变种之间的H2O2预告的处理的Cddetoxification和反应有显著差别,这可以被推测。
简介:Dustemissionsfromsmelters,asamajorcontributortoheavymetalcontaminationinsoils,couldseverelyinfluencesoilquality.Downwindsurfacesoilswithin1.5kmofazincsmelter,whichwasactivefor10yearsbutceasedin2000,inMaguTown,GuizhouProvince,ChinawereselectedtoexaminePb,Zn,andCdconcentrationsandtheirfractionationalongadistancegradientfromazincsmelter,andtostudythepossiblee?ectsofPb,Zn,andCdaccumulationonsoilmicroorganismsbycomparingwithareferencesoillocatedatadownwinddistanceof10kmfromthezincsmelter.Soilswithin1.5kmofthezincsmelteraccumulatedhighlevelsofheavymetalsZn(508mgkg-1),Pb(95.6mgkg-1),andCd(5.98mgkg-1)withlowratiosofZn/Cd(59.1-115)andPb/Cd(12.4-23.4).Compositepollutionindices(CPIs)ofsurfacesoils(2.52-15.2)were3to13timeshigherthanthereferencesoils.Inmetalaccumulatedsoils,exchangeablepluscarbonate-boundfractionsaccountedformorethan10%ofthetotalZn,Pb,andCd.Thesaturationdegreeofmetals(SDM)insoilswithin1.5kmofthesmelter(averaging1.25)wassixtimeshigherthanthatofthereferencesoils(0.209).Asmallersoilmicrobialbiomasswasfoundmorefrequentlyinmetalaccumulatedsoils(85.1-438μgCg-1)thaninreferencesoils(497μgCg-1),andanegativecorrelation(P<0.01)ofsoilmicrobialbiomasscarbontoorganiccarbonratio(Cmic/Corg)withSDMwasobserved.Microbialconsumptionofcarbonsourceswasmorerapidincontaminatedsoilsthaninreferencesoils,andashiftinthesubstrateutilizationpatternwasapparentandwasnegativelycorrelatedwithSDM(R=-0.773,P<0.01).Consequently,dustdepositedPb,Zn,andCdinsoilsfromzincsmeltingwerereadilymobilized,andweredetrimentaltosoilqualitymainlyinrespectofmicrobialbiomass.
简介:Inordertoexplorethefeasibilityofcryopreservationondormantforesttreeseeds,theseedviabilityof4species(Malusbaccata,Prunusdavidiana,P.armenicaandAmygdalupersica)withmoisturecontent(MC)changingwastestedafter30-daystorageinliquidnitrogenof-196℃.Theresultsshowedthatallthe4speciesofseedscankeepastableviabilityaftercryopreservation.Intheprocedure,theoptimumMCwas48g/kgforM.baccata,27g/kgforP.davidiana,31g/kgforP.armenicaand45g/kgforA.persica,...