学科分类
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6 个结果
  • 简介:Anewmethodwasdescribedforusingarecurrentneuralnetworkwithbiasunitstopredictcontactmapsinproteins.Themaininputstotheneuralnetworkincluderesiduespairwise,residueclassificationaccordingtohydrophobicity,polar,acidic,basicandsecondarystructureinformationandresidueseparationbetweentworesidues.Inourwork,adatasetwasusedwhichwascomposedof53globulinproteinsofknown3Dstructure.Anaveragepredictiveaccuracyof0.29wasobtained.Ourresultsdemonstratetheviabilityoftheapproachforpredictingcontactmaps.

  • 标签: 循环神经网络 蛋白质 预测 血红素 三维结构
  • 简介:Thethree-dimensional(3D)structurepredictionofproteinsisanimportanttaskinbioinformatics.Findingenergyfunctionsthatcanbetterrepresentresidue-residueandresidue-solventinteractionsisacrucialwaytoimprovethepredictionaccuracy.Thewidelyusedcontactenergyfunctionsmostlyonlyconsiderthecontactfrequencybetweendifferenttypesofresidues;however,wefindthatthecontactfrequencyalsorelatestotheresiduehydrophobicenvironment.Accordingly,wepresentanimprovedcontactenergyfunctiontointegratethetwofactors,whichcanreflecttheinfluenceofhydrophobicinteractiononthestabilizationofprotein3Dstructuremoreeffectively.Furthermore,afoldrecognition(threading)approachbasedonthisenergyfunctionisdeveloped.Thetestingresultsobtainedwith20randomlyselectedproteinsdemonstratethat,comparedwithcommoncontactenergyfunctions,theproposedenergyfunctioncanimprovetheaccuracyofthefoldtemplatepredictionfrom20%to50%,andcanalsoimprovetheaccuracyofthesequence-templatealignmentfrom35%to65%.

  • 标签: 狂犬病 功能 蛋白识别 疾病预防
  • 简介:China'sFreeARTProgramwasinitiatedin2002asanemergencyresponsetosaveandimprovethelivesofAIDSpatientslivingmainlyinimpoverishedruralregionsofcentralChina.WithlittleexperienceinHIV/AIDStreatmentandcareandresourcelimitations,China'seffortstoprovidewidespreadaccesstofreeantiretroviraltherapyhasbeenaprocessfraughtwithdifficulty.However,theFreeARTProgramisprogressingfromanemergencyresponsetoastandardizedtreatmentandcaresystem.Thedevelopmentofnationalguidelines,trainingprograms,alaboratorysupportnetwork,anationalpatientdatabase,programsforspecialpopulationssuchaschildrenandpatientslivingwithcoinfections,andoperationalresearchhasimprovedthescopeandqualityofthefreetreatmentprogram.AsofJune30,2005,atotalof19,456patientsin28provinces,autonomousregions,andspecialmunicipalitieshadreceivedfreeART.ChallengesstemmingfromthenatureofChina'shealthsystemandpatientpopulationpersist,butwithstronggovernmentsupportandadiversesetofresources,Chinahasthecapacitytoovercomethesechallengesandtoprovidenationwideaccesstohighqualitytreatmentandcare.

  • 标签: 爱滋病 中国 治疗费用 农村 公共卫生
  • 简介:IncubationofdinoflagellateCrythecodiniumcohniichromosomesincytoplasmicextractsofunfertilizedXenopuslaeviseggsresultedinchromosomesdecondensationandrecondensation,nuclearenvelopeassembly,andnuclearreconstitution.DinoflagellateCrythecodiniumcohniiisakindofprimitiveeukaryotewhichpossessesnumerouspermanentlycondensedchromosomesanddiscontinuousdouble-layerednuclearmembranethroughoutthecellcycle.Theassemblednuclei,beingsurroundedbyacontinuousdoublemembranecontainingnuclearporesandtheuniformlydispersedchromatinfibersaremorphologicallydistinguishablefromthatofDinoflagellateCrythecodiniumcohnii.However,incubationofdinoflagellateCyrthecodiniumcohniichromosomesintheextractsfromdinoflagellateCrythecodiniumcohniicellsdoesnotinducenuclearreconstitution.

  • 标签: 纯化 甲藻染色体 爪蟾 蛙卵无细胞提取物 核重组 细胞核