简介:FurnitureofHongmu,creamofChineseculture,hasalonghistoryandenjoysgreatpopularityforitsclassicappearanceandelegantdisposition.Thetraditionalmethodoftheidentificationistoevaluatethemacroformandanatomicalfeatures.Inthispaper,anewmethodbasedonthedifferencesamongthechemicalcharacteristicsofbiologicalmetabolitesofsamplesisdeveloped.Intheresearch,highperformanceliquidchromatography(HPLC)fingerprintswereemployedtodiscriminatefourspeciesofHongmu,includingPterocarpusindicus,Pterocarpusmacarocarpus,Dalbergiamelanoxylon,andDalbergialouvelii.Theresultsshowedthat,accordingtotheretentiontimeandrelativepeakareaofdifferentcomponentsinwoodextracts,thedistinctioncanbeeasilymadebetweendifferentspecies.BasedontheHPLCfingerprints,similaritiesbetweensampleswereevaluatedbycorrelationcoefficients.Theresultsweregreaterthan0.81betweenthesamespecies,andlessthan0.21betweendifferentspecies,whichshowedweakcorrelationbetweendifferentspecies.Inordertoestimatetheoverallfeaturesofthefingerprints,hierarchicalclusteringandprincipalcomponentanalysis(PCA)wereusedandtheresultsshowedpronouncedclusteringeffectamongthesamespecies.TheinvestigationindicatedthatpatternrecognitioncouldaccuratelyreflectthesignificantdifferencesbetweendifferentspeciesofHongmu,andcanbeusedasareferenceforqualitycontrol.
简介:摘要目的建立以高效液相色谱法测定人血清中甲氨蝶呤浓度的方法。方法采用Shim-packVP-ODS(150mm×4.6mm,5μm)的色谱柱;流动相乙腈—0.3%磷酸(1585,V/V,用三乙胺调pH2.5);紫外检测波长为302nm;柱温40℃;流速0.8mL•min-1。结果MTX在0.1~100.0μg•mL-1质量浓度内线性关系良好,Y=0.2+30174X,r=0.9994;MTX低、中、高(0.25μg•mL-1、12.50μg•mL-1、100.00μg•mL-1)3种浓度的平均回收率分别为104.0%、102.3%、102.5%。日内、日间RSD均<3%,最低血清检测浓度50ng•mL-1。结论高效液相色谱法测定甲氨喋呤的血药浓度灵敏度高,结果准确可靠,操作简便,能满足MTX血清浓度的测定。
简介:摘要目的探讨高效液相色谱法测定川黄芩中的黄芩苷含量的方法学特点。方法取川黄芩样品粉末(共5批),比较70%乙醇、稀乙醇、甲醇、50%甲醇对其黄芩苷的提取效率。取黄芩苷对照品溶液10ul,连续5次注入液相色谱仪,研究黄芩苷进样量与峰面积的线性关系,并进行精密度试验、重复性试验、加样回收实验、耐用性实验,最后进行样品测定。结果测定波长选定为277nm,选用甲醇-水-磷酸(47530.2)作为测定流动相,70%乙醇为本品的提取溶剂,以回流提取3小时为提取方法。黄芩苷在进样量为30.31~1212.4μg之间时,与峰面积线性关系良好。结果表明,该法精密度、重现性、准确度及耐用性均较好。结论高效液相色谱法定量测定川黄芩中的黄芩苷,方法简单、准确度高。
简介:摘要目的建立荭草中齐墩果酸的定量分析方法。方法采用高效液相色谱法定量齐墩果酸,色谱柱KromasilC18反相柱(5µm×250mm×4.6mm),流动相乙腈-磷酸水溶液(75∶25),柱温为35℃,流速1.0ml/min,检测波长210nm。结果HPLC法测定结果显示,色谱峰形好,分离度高。齐墩果酸在0.4~10µg(r=1)呈良好的线性关系;供试品在48h内稳定(RSD=1.01%);该方法精密度高(RSD=1.10%),重复性良好(RSD=0.88%),平均加样回收率为101.31%(RSD%=2.11%)。结论所建立的方法适合作为荭草的含量测定方法。
简介:摘要目的建立高效液相色谱法测定二仙颗粒中淫羊藿苷的含量测定方法。方法固定相KromasilC18(5μm,250×4.6mm);流动相甲醇-0.4%磷酸溶液(5545);检测波长270nm,柱温35℃。结果淫羊藿苷在0.072~0.36μg范围内线性关系良好(r=0.9999),平均回收率为99.18%(n=5),RSD为1.89%。结论结果准确,重复性好,可用于该制剂的质量控制。
简介:Thepresentworkisaimedtostudythepharmacokineticparametersofoptimizedrepaglinidefloatingdrugdeliverysystem(FDDS)by24factorialdesigns,followedbycomparisonwithacommerciallyavailableformulation.Themaineffectsandinteractionsofformulationvariableswerestudiedbyusingnormalandparetocharts.Theoptimizedformulationshowsafickiandiffusiondrugreleasemechanism.Pharmacokineticparametersofthedesigneddrugdeliverysystemwereevaluatedinrabbitmodels.Meanwhileasimple,specifichighperformanceliquidchromatographicmethodwasdevelopedandvalidatedasperbiopharmaceuticalspecifications,thelinearitywasobservedattherangeof110-550ng/mL(r2=0.999).Byusingmethanol-phosphatebuffer(pH2.5)(70:30,v/v)asmobilephaseattheflowrateof1.0mL/minthevalidationshowsabetterretentiontimeof5.2minforrepaglinide.AndthesamevalidationmethodwasusedforpharmacokineticprofileanalysisofrepaglinidemarketedproductsandFDDS.Thecomparativepharmacokineticresultssuchastmax,half-life,areaunderthecurve,meanresidencetimeswereincreasedsignificantlyfortherepaglinideinFDDSthanthemarketedproductofrepaglinideexceptCmaxandeliminationrateconstant.