学科分类
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4 个结果
  • 简介:Leber'scongenitalamaurosis(LCA)andrecentgenetherapyadvancementfortreatinginheritedretinopathieswereextensiveliteraturereviewedusingMEDLINE,PubMedandEMBASE.Adeno-associatedviralvectorswerethemostutilisedvectorsforoculargenetherapy.Conephotoreceptorcellsmightuseanalternatepathwaywhichwasnotreliantoftheretinalpigmentepithelium(RPE)derivedretinoidisomerohydrolase(RPE65)toaccessthe11-cisretinaldehydechromophore.Researcheffortsdedicatedontheprogressionofagene-basedtherapyforthetreatmentofLCA2.Suchgenetherapyapproacheswereextremelysuccessfulincanine,porcineandrodentLCA2models.TherecombinantAAV2.hRPE65v2adenoassociatedvectorcontainedtheRPE65cDNAandwasreplicationdeficient.ItsinvitroinjectionintargetcellsinducedRPE65proteinproduction.Thegenetherapytrialsthatweresofarconductedforinheritedretinopathieshavegeneratedpromisingresults.PhaseIclinicaltrialstocureLCAandchoroideremiademonstratedthatadeno-associatedviralvectorscontainingRPEgenesandphotoreceptorsrespectively,couldbesuccessfullyadministeredtoinheritedretinopathypatients.AphaseIIItrialispresentlyongoingandifsuccessful,itwillleadthewaytoadditionalgenetherapyattemptstocuremonogenic,inheritedretinopathies.

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  • 简介:Background:Thehospitalizationburdenofcongenitalheartdisease(CHD)atthenationalorregionallevelinChinaremainsunknown.WeaimedtoevaluatetherecentpatientcharacteristicsandtemporaltrendsofhospitalizationsforCHDinBeijing.Methods:PatientshospitalizedforCHDinBeijingfrom2007to2011wereidentifiedfromtheHospitalDischargeInformationSystem.Patientcharacteristicswererecorded,andtrendsinhospitalizationrateswereanalyzedbyPoissonregressionafteradjustmentforageandsex.Results:Atotalof53,064patientswereadmittedforCHDinBeijingduringthestudyperiod,amongwhom50.5%werechildrenyoungerthan5years,30.0%wereadults,18.5%hadsevereCHD,86.9%werenonpermanentBeijingresidents,and81.3%wereadmittedtocardiac-specifictertiaryhospitals.Thehospitalizationrateincreasedfrom10.2per100,000populationin2007to12.4per100,000populationin2011,representingasignificantincreaseafteradjustmentforageandsex(P=0.009).However,thetrendsvariedindifferentgroups.Therateshalvedin2011forbothnonsevereCHDandsevereCHDcomparedwiththeratesin2010,whereasincreasingtrendswerenoticedinchildrenaged1–4yearsandadultsfornonsevereCHDacrossthestudyperiod.Conclusions:TheoverallrateofCHDhospitalizationhasbeenincreasinginBeijing,whereasnotallpatientgroupshavethesametrend.Thehospitalizationratedecreasedamonginfants,whiletheratefornonsevereCHDincreasedsignificantlyamongadults.Thesefindingswillbeofimportanceforfutureallocationofhealthresources.

  • 标签: CONGENITAL HEART DISEASE HOSPITALIZATION TREND
  • 简介:AIMTo鉴别在透明婴儿透镜和先天的cataract.METHODSLens相关的miRNAs的中央上皮的透镜相关的microRNAs(miRNAs)的表示从PubMed被检索数据库。在透明婴儿透镜和先天的奔流的这些miRNAs的表示层次被茎环颠倒抄写聚合酶链反应(RT-PCR)决定。人权保护的算法被用来预言这些差别的目标基因表示了miRNAs。目标mRNA是validated.RESULTSSix透镜相关的miRNAs从屏蔽PubMed被检索数据库。在透明婴儿透镜的最丰富的miRNA根据茎环RT-PCR是miR-184。miR-182在先天的奔流是起来调整的。相反地,miR-204和miR-124是下面调整的。miR-204比miR-124在表示展出了更重要的减少。另外,Meis2被预言是用人权保护的算法的miR-204的目标。miR-204mimic/antagomirtransfection实验建议了在miR-204的表示之间的否定关联,建议他们在先天的奔流的致病的参与并且miR-182,miR-204和miR-124的Meis2.CONCLUSIONThe表示层次在透明婴儿透镜和先天的奔流的中央上皮之间不同。miR-204可以经由silencingMeis2行动调整透镜开发和先天的奔流形成。

  • 标签: 透镜相关的 miRNAs 先天的奔流 miR-204 Meis2
  • 简介:AIMTo用角膜的地形学和共焦的系统决定先天的aniridickeratopathy(CAK)的典型角膜的变化,并且那可能在早diagnosis.METHODSPatients帮助识别特征CAK,健康控制题目包括轴的长度经历了详细眼的考试,角膜的厚度,眼泪电影状况,角膜的地形学,并且在vivo扫描激光共焦的显微镜学(IVCM).RESULTSIn早阶段aniridickeratopathy,我测试的Schirmer(坐),分散tim另外,显著地,更多的眼睛与控制组相比展出了扁平的角膜。煽动性的树枝状的房间在aniridic上皮是在场的,与相对控制的显著地增加的密度(P<0.05)。栅如脊的特征和反常房间形态学从十六个CAK盒子在六被观察。在中央角膜区域,aniridic角膜有神经density.CONCLUSIONThese处于边线状况在角膜的形态学改变的增加的subbasal能有用证实CAK的诊断。

  • 标签: aniridic keratopathy 角膜的地形学 共焦的显微镜学