简介：ＣｈａｎｇｉｎｇｔｈｅＤａｂａＭｏｕｎｔａｉｎｓｗｉｔｈＷｉｓｄｏｍａｎｄＳｗｅａｔＴｈｅｆｏｒｍｅｒｒｅｓｉｄｅｎｃｅｏｆＤｅｎｇＸｉａｏｐｉｎｇｉｎＧｕａｎｇ＇ａｎＣｏｕｎｔｙ．ＡｌｌａｒｏｕｎｄｔｈｅＤａｔａＭｏｕｎｔａｉｎａｒｅａｔｈｅｐｅｏ...

简介：无

简介：Objective:Toobservethetherapeuticeffectofacupuncturetherapyfor120casesofhyperplasiaofmammaryglands.Methods:Thesepatientswereclassifiedintoliver-qi-stagnationtype(n=53),phlegm-coagulationtype(n=30)andThoroughfare-Conceptionvesselmaladjustmenttype(n=37).AcupointsusedwereQimen(期门LR14),Wuyi(屋翳ST15),Weishu(胃俞BL21),etc..Acupuncturetreatmentwasconductedoncedailyand30treatmentsconstitutedonetherapeuticcourse.Results:After3coursesoftreatment,ofthe53,30and37casesoftheliver-qi-stagnation,phlegm-coagulationandThoroughfare-Conceptionvesselmaladjustmenttypes,43(81.1%),24(80.0%)and29(78.4%)werecured,7(13.2%),5(16.7%)and5(13.5%)markedlyimproved,3(5.7%),1(3.3%)and2(5.4%)improvedrespectively,and1(2.7%)ofThoroughfare-Conceptionvesselmaladjustmenttypefailed.Atwo-years'follow-upshowedthat3ofthecured92caseshadarelapse,andafter2morecoursesoftreatment,theywerecuredagain.Conclusion:Acupuncturetherapyiseffectiveforthetreatmentofhyperplasiaofmammaryglands.

简介：无

简介：AbstractBackground:Sweat secreted by eccrine sweat glands is transported to the skin surface through the lumen. The eccrine sweat gland develops from the initial solid bud to the final gland structure with a lumen, but how the lumen is formed and the mechanism of lumen formation have not yet been fully elucidated. This study aimed to investigate the mechanism of lumen formation of eccrine gland organoids (EGOs).Methods:Human eccrine sweat glands were isolated from the skin for tissue culture, and the primary cultured cells were collected and cultured in Matrigel for 14 days in vitro. EGOs at different development days were collected for hematoxylin and eosin (H&E) staining to observe morphological changes and for immunofluorescence staining of proliferation marker Ki67, cellular motility marker filamentous actin (F-actin), and autophagy marker LC3B. Western blotting was used to detect the expression of Ki67, F-actin, and LC3B. Moreover, apoptosis was detected using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis assay kit, and the expression of poly (ADP-ribose) polymerase and Caspase-3 was detected by Western blot. In addition, 3-methyladenine (3MA) was used as an autophagy inhibitor to detect whether the formation of sweat glands can be effectively inhibited.Results:The results showed that a single gland cell proliferated rapidly and formed EGOs on day 4. The earliest lumen formation was observed on day 6. From day 8 to day 14, the rate of lumen formation in EGOs increased significantly. The immunofluorescence and Western blot analyses showed that the expression of Ki67 gradually decreased with the increase in days, while the F-actin expression level did not change. Notably, the expression of autophagy marker LC3B was detected in the interior cells of EGOs as the apoptosis signal of EGOs was negative. Compared with the control group, the autophagy inhibitor 3MA can effectively limit the formation rate of the lumen and reduce the inner diameter of EGOs.Conclusion:Using our model of eccrine gland 3D-reconstruction in Matrigel, we determined that autophagy rather than apoptosis plays a role in the lumen formation of EGOs.

简介：类Polistes的黄蜂被认为钥匙到在Hymenoptera理解社会行为的进化。几研究证明了组织昆虫社会的发展被象外分泌腺一样的结构的进化伴随，它变得专业化执行特定的功能。这篇文章调查低亚硫酸钠的极端结构、细胞化学的特征Polistes的咽头的腺多色。这些腺在社会蜜蜂深入地被学习了，在他们仅仅在护士发生并且生产王浆的地方。我们的结果表明这些腺基本上没在个人之中或在性之间变化。他们被球形的房间，有一个大原子核的各个和开发得好的不平的endoplasmic组成。分泌物泡是丰富的，但是类脂化合物微滴没被观察显示这些腺不能在外激素合成有一个角色。酸性磷酸酶在lysosomes被检测，并且也在cytosol释放，但是不似乎与房间死亡相关。因此，我们的结果建议低亚硫酸钠P的咽头的腺。多色不能有一个专业化社会角色，但是能生产消化酶。

简介：AbstractObjective:This study was performed to explore the conversion of adipose-derived stem cells (ADSCs) into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland (hSG) cells were isolated, cultured, and identified. The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells. Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers.Results:The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers (cytokeratins 7, 14, and 18).Conclusion:These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation. ADSCs may serve as a potential source of cells for sweat gland regeneration.

简介：Objective:Mucoepidermoidcarcinoma(MEC)isthemostcommonprimarymalignancyofthesalivaryglands.Insulin-likegrowthfactor-IImRNA-bindingprotein-3(IMP3)isanimportantprognosticfactorinsomecancersandatoolthatdifferentiatesbetweenbenignandmalignantpancreaticlesions.ThisstudyaimedtoidentifyarelationshipbetweentheexpressionofIMP3andtheoutcomeofsalivaryglandMEC,aswellastodifferentiateMECfrompleomorphicadenoma(PA).Methods:Tissuespecimensfrom70casesofsalivaryglandMEC,40casesofPA,and10caseswithnormalsalivaryglandwereexaminedimmunohistochemicallyforIMP3.TheassociationamongtheexpressionofIMP3,clinicopathologicalcharacteristicsandpatient'ssurvivalwasassessed.Results:IMP3waspresentin51.4%ofMECbutabsentinPAandnormalsalivaryglandtissues.IMP3expressionwasassociatedwithage>60years,submandibularglandtumors,tumorsize>4cm,high-gradetumors,lymphnodemetastasis,involvementofsurgicalmargins,perineuralinvasion,distantmetastasis,advancedTNMstage,tumorrelapse,anddeath(P<0.05).IncreasedexpressionofIMP3,tumorsofthesubmandibulargland,andlymphnodemetastasiswereindependentprognosticfactorsofdisease-freesurvival(DFS).Inaddition,IMP3wasastrongpredictorofoverallsurvival(OS)togetherwithdistantmetastasisandintermediateandhigh-gradetumors.Conclusions:IMP3expressionishighlyimportantinevaluatingtheoutcomeofMEC.IMP3canbeusedtodifferentiateMECfromPAofsalivaryglands.

简介：

简介：无

简介：