简介:MultitoxinBt-cropsexpressinginsecticidaltoxinswithdifferentmodesofaction,forexample,CryandVip,areexpectedtoimproveresistancemanagementintargetpests.WhileCry1Aresistancehasbeenrelativelywellcharacterizedinsomeinsectspecies,thisisnotthecaseforVip3A,forwhichnomechanismofresistancehasyetbeenidentified.HereweappliedHT-SuperSAGEtoanalyzethetranscriptomeoftheguttissueoftobaccobudwormHeliothisvirescens(F.)laboratory-selectedforVip3Aaresistance.Fromatotalof1324252sequencereads,5895126-bptagswereobtainedrepresenting17751nonsingletonuniquetranscripts(UniTags)fromgeneticallysimilarVip3Aa-resistant(Vip-Sel)andsusceptiblecontrol(Vip-Unsel)strains.Differentialexpressionwassignificant(≥2.5foldor≤0.4;P<0.05)for1989sequences(11.2%oftotalUniTags),where420representedoverexpressed(OE)and1569underexpressed(UE)genesinVip-Sel.BLASTNsearchesmapped419UniTagstoH.virescenssequencecontigs,ofwhich,416(106OEand310UE)wereunambiguouslyannotatedtoproteinsinNCBInonredundantproteindatabases.GeneOntologydistributed345ofannotatedUniTagsin14functionalcategorieswithmetabolism(includingserine-typehydrolases)andtranslation/ribosomebiogenesisbeingthemostprevalent.AUniTaghomologoustoaparticularmemberoftheREsponsetoPAThogen(REPAT)familywasfoundamongmostoverexpressed,whileUniTagsrelatedtotheputativeVip3Aa-bindingribosomalproteinS2(RpS2)wereunderexpressed.qRT-PCRofasubsetofUniTagsvalidatedtheHT-SuperSAGEdata.ThisstudyisthefirstprovidinglepidopteranguttranscriptomeassociatedwithVip3Aaresistanceandafoundationforfutureattemptstoelucidatetheresistancemechanism.
简介:最近,建设部试点项目大连锦绣园采用的一种HT-800双组分墙体保温材料,经1年跟踪监测后,证明该材料施工方便,与保温板材相比,减少裁剪、拼装、粘接、嵌缝等工艺,节省施工费用,压缩工期;成型后不空鼓、裂纹、脱落;使用时,冬不结露、返凉,夏不返热;业主入住后进行装修时,也不会损坏墙体保温效果。
简介:Characteristicsofiontemperaturemeasuredwithcharge-exchangerecombinationspectroscopy(CXRS)werestudiedinOhmic,lower-hybrid-wave(LHW)drivenandion-cyclotron-resonance-frequency(ICRF)heatedplasmasinHT-7.TheresultsindicatethatthecentraliontemperatureTi0followstheone-thirdpowerlawintheproductofcentralline-averageddensityneandplasmacurrentIpinOhmicdischargesandisthereforeconsistentwiththeArtsimovichscalinglawTi0=K·(Ip·Bt·ne·R2)1/3.ItisshownthatthereisanappreciableincreaseofiontemperatureduringtheoperationwithbothLHWandICRFandthattheincrementofiontemperatureinthoseshotsismainlyduetotheenergytransferviacollisionsbetweenionsandelectronsratherthatbydirectheatingoftheions.
简介:SawtoothcontrolexperimentswereperformedonHT-7withalimiterconfigurationandontheExperimentalAdvancedSuperconductingTokamak(EAST)withadoublenullcon-figuration.Thesawtoothperiodcanbemodifiedbylowerhybridwave(LHW)andioncyclotronresonancefrequency(ICRF).Differentsawtoothbehaviorwasobservedwiththesameplasmadensity,LHWpowerbutdifferentplasmacurrentsonHT-7.TherewasadwelltimebetweenLHWaddedandthesawtoothstabilizationonHT-7whilethesawtoothstabilizationimmediatelyhappenedwhenLHWpowerwasinjectedonEAST.Thepossiblemechanismofthesawtoothcontrolisdiscussedinthispaper.Alltheexperimentalresultshaveprovedthatpowerdepositionoftheradiofrequency(RF)shouldbethekeyfactorforsawtoothcontrol.
简介:Experimentsonlowerhybridwave(LHW)couplingwereinvestigatedintheHT-7tokamak.GoodcouplingofLHWplasmahasbeendemonstratedatdifferentconditionsintheHT-7tokamak.RelevantresultshaveprovedthatLHW-plasmacouplingisaffectedbythephasedifferencebetweenadjacentwaveguides.Furthermore,theedgedensityaroundthegrillandrelevantcouplingcanbeadjustedbychangingtheplasmalineaveragedensityorthegapvaluebetweentheLHgrillandthelastclosedfluxsurfaces(LCFS).ItisfoundthatthecouplingofLHWsbecomespoorwhentheedgedensityaroundtheLHgrillislargeenoughintheHT-7tokamak,andthatcouplingremainsgoodwithaproperedgedensity.WithincreasingLHWpower,itisalsofoundthatthereflectioncoefficients(RCs)increaseduetonon-lineareffectsunderconditionsoflowedgerecycling,butcandecreaseunderhighedgerecycling.Theedgedensitydependsmainlyonthecompetitionbetweentheponderomotiveforce(PMF)andtheedgerecyclingintensityintheHT-7tokamak.
简介:SawtoothactivityonHT-7tokamakhasbeeninvestigatedexperimentallymainlybyusingsoftx-raydiodearrayandmagneticprobes.Theirbehaviorsandoccurrencesarecorrelatedcloselytothedischargeconditions:theelectrondensityNe,theelectrontemperatureTe,thesafetyfactorqaonplasmaboundaryandwallconditionetc.Whencentralline-averagedelectrondensityNe(0)isover2.0×1013cm-3,majorsawtoothactivityemergeswithaperiodofupto6.5msandafluctuationamplitudeofupto2~30%ofSXRradiationsignal.Insomecasessuchasthesafetyfactorbetween4.2~4.7andZeff=3.0~6.0,amonstersawtoothactivityoftenemergeswithoutapparentdeteriorationofplasmaconfinementandwithoutmajordisruption.Duringtheseevents,abundantMHDphenomenaareobservedincludingpartialsawtoothoscillations.Inthispaper,theobservedsawtoothbehaviorsandtheirdependenceontheandtheirdependencedensityNeandwallconditioninohmicallyheatedplasmaareintroduced,theresultsarediscussedandpresented.
简介:TheICRF(IonCyclotronRangeFrequency)cleaningtechniquehasbeenusedasaroutinewallcleaningmethodintheHT-7superconductingtokamak.Inawiderangeoftoroidalfield,thcremovalrateofresidualgasbyICRFcleaningwasabouttwentytimeshigherthanthatofglowdischargecleaning(GDC).AtdifferentgaspressureandRFpowerlevels,theICRFcleaningisstudiedcarcfully.Agoodimpurityclcaningeffectandaveryhighhydrogenremovalratewereobtained.Theremovalrateofhydrogenby5kWICRFcleaningachievedwas1.6×10-5Torr.1/s.AndtherelationshipsamongpressureP,outgassingrateQ,atomiclayersLabsorbedonsurfaceandthecleaningmodewerediscussedbriefly.
简介:Thispaperreportsforthefirsttimeanexperimentalstudyonthepowerdepositionprofileoftheioncyclotronrangefrequencies(ICRF)powerdepositingonelectronsinHT-7.ThefastFouriertransform(FFT)analysisandthebreakinslope(BIS)methodareutilizedtoobtaintheinformationofthepowerdeposition.Theresultsindicatethattheelectronswereheateddirectly,andtheelectronsabsorbedaround20%oftheinputpowerofthedischargeofinterest.
简介:摘要目的5-羟色胺受体(5-HT1A和5-HT3受体)在七氟烷诱发老龄大鼠脑神经毒性中的作用。方法清洁级健康雄性SD大鼠24只,18~20月龄,体重600~750 g,采用随机数字表法分为3组(n=8):分别吸入50%O2(C组)、1.5%七氟烷+50%O2(LS组)及3%七氟烷+50%O2(HS组),持续2 h。于吸入七氟烷前1 d及结束后1 d时行旷场实验,记录中央格停留时间、跨格次数和站立次数;于吸入七氟烷前6 d时及结束后第1天行水迷宫实验,记录逃避潜伏期、游泳总距离和穿越平台次数。行为学实验结束即刻取海马组织,采用RT-PCR法和免疫组化法检测5-HT1A和5-HT3受体的mRNA表达水平及阳性细胞数。结果与C组比较,HS组中央格停留时间延长,跨格次数及站立次数减少,逃避潜伏期延长,游泳总距离增加,穿越平台次数减少,5-HT1A和5-HT3受体的mRNA表达下调,阳性细胞数减少(P<0.05)。结论七氟烷诱发老龄大鼠脑神经毒性的机制可能与下调5-HT1A和5-HT3受体活性有关。