简介:Mt.QomolangmaistheloftiestmountainatthetopofHimalayas;themountainpeakisshapedlikeapyramidandpenetratesdirectlyintotheskyofferingaspectacularview.
简介:TheTechniqueofStoneMa-sonryatRdoSbusLungPaThetechniqueofmasonryasusedinconstructioninTibetcanbedatedbacktotheYumbulagangduringtheTuboKingdomanditstillprevailsalloverTibet.Buildingsofallkindsontheplateau,suchaswatchtowersandcivilianresidences,haveadoptedthistechnique.AvarietyofbuildingsshowthishistoricTibetaninheritanceanditsevolution.ToapplythisstonemasonrytechniquetobuildingconstructioncloselyConnectswiththe
简介:AVillageattheFootofMt.MeliZHOUZHENGSkirtedbytheLan-cangjiangandNu-jiangriv-ers,MeliMountainstandsma-jesticallyattheborderbetw...
简介:WhyethnicTibetansareneverdauntedbythehardshipstheysufferwhilemakingpilgrimagestosacredmountainsandlakesbywalkingroundthem?IputthisquestiontoamanwhohadwalkedroundthesacredMt.KhawahGepohineasternTibet28times.Hereishisanswer:"Toendurethosehardshipsduringthepilgrimages,ImeantopunishmyselfforthesinsIhavecommitted,inordertogainfreedomfromanxietyandincreasethevirtuesinmysoul,myinnerworld."Withhelpfromsomelamas,Ilearnedbyheartthewordsofprayerpilgrimskeeputteringwhilewalkingroundthesacredmountain:
简介:Theimmuneefficiencyofarecombinantadenovirustype5withtype35fibercontainingHIV-1gaggene(rAd5/F35-mod.gag)wasinvestigatedinBALB/cmice,inwhichtherAd5/F35-mod.gagwasfirstlyidentifiedwithPCR,thentransfectedto293cellsandtheinvitroexpressionlevelofGagproteinwasdeterminedbyWesternblottingandindirectimmuno-fluorescentassay.MicewereimmunizedwithintramuscularinjectionsofrAd5/F35-mod.gag,rAd5-mod.gagorDNAandwereboostedafter3weeks.Totesttheeffectofpre-existinganti-viralimmunityonimmunization,micewerealsoinjectedwithAd5-GFPvectorandthenimmunized4and7weekslaterwithAd5/F35-mod.gagvector.TheP24-specificIgGantibodyinseraofimmunizedmicewasdeterminedbyELISAandthespecificcytotoxicTlymphocyte(CTL)responsewasassayedbyintracellularcytokinestaining.ItwasdemonstratedthattherAd5/F35-mod.gagvectorcouldexpressefficientlytheHIVGagproteinin293cellsinvitroandinducestrongHIV-specificimmuneresponsesinvivo.ThestrongestCTLandserumIgGresponseoccurredwhenmicewereimmunizedtwicewithinjectionofrAd5/F35alone,buttheanti-Ad5antibodyafterprimaryinfectionwithadenoviruscouldinhibitthespecificimmuneresponsesinducedbyrAd5/F35vector.ItisconcludedthatsingleimmunizationwithrecombinantadenovirusrAd5/F35-mod.gagcaninducespecificCTLandserumIgGantibodyresponsesinmice,buttheimmunogenicityofrAd5/F35iscomparablyweakerthanthatofrAd5.