简介：ObjectiveTheaimofthisstudyistoinvestigatetheeffectoftransfectedhTERTgeneoncellapoptosisofnewbornratcochlearbasilarmembranecells(CBMCs).MethodsCBMCsisolatedfromnewbornratcochlearweretransfectedusingaplasmidcontaininghumantelomerasereverasetranscriptasegene(pCI-neo-hTERT),andwerescreenedusingG418toobtainstabletransfectedcelllines.Cellapoptosisratewasanalyzedbyflowcytometry.hTERTandapoptosisrelatedgenesexpressionweredetectedbyreversetranscriptasepolymerasechainreaction(RT-PCR).ResultshTERTgeneexpressionwasdetected72hoursaftergenetransfectionintransfectedcells.TheapoptoticrateoftransfectedCBMCssignificantlyreduced.Expressionofapoptosisrelatedgenescorrespondinglychanged.ConclusionTransfectionofhTERTgeneleadstoreducedapoptosisrateinnewbornratCBMCs.andlowerexpressionofapaf1,Caspase3andBCL2intransfectedcellsascomparedtothatofnormalCBMCs.

简介：AbstractObjective:To compare and analyze the pass rate and screening strategy of hearing rescreening for newborns with high risk factors.Methods:Retrospective chart review of high-risk newborns who failed their initial newborn hearing screen and subsequently underwent secondary hearing tests from June 2011 to June 2018 in Guangzhou Women and Children's Medical Center were performed.Results:Eight hundred and sixty-eight newborns with high risk factors were included in the study. The 57-70 days (83.5%) and 71-84 days (83.4%) group had the highest pass rate compared with 42-56 days (75.8%) and < 42 days (68.3%) group. As for different screening strategies, the pass rate of OAE(otoacoustic emissions), AABR (auto auditory brainstem response) and OAE + AABR was the highest in 57-70 days group and 71-84 days group, respectively. The OAE + AABR had the lowest pass rate compared to the other two modalities. When the pass rate was compared as different risk factors, the 57-70 days and 71-84 days group also had the highest pass rate compared with 42-56 days and < 42 days group and the pass rate had no significant differences among various risk factors group.Conclusion:Our results showed that all the pass rate of OAE, AABR and OAE+ AABR was the highest in 57-70 days group and 71-84 days group with significant difference, suggesting that the delayed screening time (>57 days) may increase the re-screening pass rate and reduce anxiety of parents, which is of great significance for clinical work.

简介：ObjectiveTopresentanexperimentalmethodthatallowsisolationofgreaterepithelialridge(GER)andlesserepithelialridge(LER)cellsfrompostnatalratcochleaeusingacombinatorialapproachofenzymaticdigestionandmechanicalseparationandtoinvestigatearetrovirus-mediatedgenetransfertechniqueforitspossibleutilityinimmortalizationoftheGERandLERcelllines,inanefforttoestablishaninvitromodelsystemofhaircelldifferentiation.MethodsGERandLERcellsweredissectedfrompostnatalratcochleaeandimmortalizedbytransferringtheSV40largeTantigenusingaretrovirus.Theestablishedcelllineswereconfirmedthroughmor-phologyobservation,immunnocytochemicalstainingandRT-PCRanalysis.TheHath1genewastransferredintothecelllinesusingadenovirus-mediatedtechniquestoexploretheirpotentialtodifferentiateintohaircells.ResultsTheestablishedcelllineswerestablymaintainedformorethan20passagesanddisplayedmanyfeaturessimilartoprimaryGERandLERcells.Theygrewinpatchesandassumedapolygonalmorphology.ImmunostainingshowedlabelingbySV40largeTantigenandIslet1(aspecificmarkerforGERandLER).AllpassagesofthecelllinesexpressedSV40largeTantigenonRT-PCRanalysis.Thecellsalsoshowedthecapabilitytodifferentiateintohaircell-likecellswhenforcedtoexpressHath1.ConclusionRetrovirus-mediatedgenetransfercanbeusedinestablishingimmortalizedprogenitorhaircelllinesinnewbornrat,whichmayprovideaninvaluablesystemforstudyinghaircelldifferentiationandregenerationfornewtreatmentofsensoryhearinglosscausedbyhaircellloss.

简介：ObjectiveUsingopticalimaginginbrainstemslices,westudiedevokedresponsesinthemousecochlear(CN)andvestibular(VN)nuclei.MethodsTheuseofopticalimagingallowedustovisualizethespatiotemporalpatternsofexcitatorypropagationintheCNandVN.OpticalrecordingscandifferentiateexcitatorypropagationintheventralCN(VCN)fromthatinthedorsalCN(DCN).Furthermore,weassessedtherelativedistributionofNMDAandnon-NMDAreceptorsintheseregionsusingtheglutamateantagonistsAPV(NMDAreceptorantagonist)andCNQX(non-NMDAreceptorantagonist)inmousebrainstemslicesduringpostnataldays1to3.ResultsTheaveragesensitivitytoAPVwas99.1%intheVCN,76.0%intheDCNand64.9%intheVN.TheaveragesensitivitytoCNQXwas0.9%intheVCN,24.0%intheDCNand35.1%intheVN.TheseresultsindicatethattheeffectofAPVdominatedintheVCN,whilebothAPVandCNQXwereeffectiveantagonistsintheDCNandVN.IntheVN,thedistributionofAPV-andCNQX-sensitivecellswasalmostcompletelyuniform.However,intheDCNthedistributionofAPV-andCNQX-sensitivecellswashighlycomplex.TheareathatwasmoresensitivetoCNQXwaslocatedinthesuperficiallayeroftheDCNwhiletheareawithahighersensitivitytoAPVwaslocatedprogressivelyinthedeeplayer.ConclusionThisopticalrecordingdatasuggeststhatthereisadifferentialdistributionofNMDAandnon-NMDAreceptormediatedneurotransmissionintheVCN,DCNandVN.

简介：摘要：我国回归社会人员重新步入社会时所遇到的最普遍的问题便是再就业，并且作为一个社会问题长期存在，涉及到人权、经济、社会治安等方面。本文意在通过设计远程面试平台，从而通过科技赋能把其运用到监狱中，以远程面试平台为载体，通过监狱提供的信息以及面试与交谈，企业以较为客观的角度更加直观地了解回归社会人员的个人能力，减少对这一群体的偏见，顺利搭建监狱和社会之间的桥梁，便利回归社会人员与企业的直接沟通，解决回归社会人员就业难的问题。同时，远程面试适用于临近回归社会人员，旨在给他们提供绿色通道，减少出狱后的社会排斥，以此解决回归社会人员就业难的问题，帮助他们走上正轨的同时降低再次犯罪率，从而减少社会中的不利因素，促进构建和谐稳定的社会关系。