简介：AbstractOxidative stress is caused by the imbalance between the generation of free radicals/reactive oxygen species (ROS) and the antioxidant defense systems, which can activate various transcription factors and affect their transcriptional pathways. Oxidative stress plays an important role in the occurrence and development of leukemia and is closely related to the treatment and prognosis of leukemia. The standard chemotherapy strategies for the pre-treatment of leukemia have many drawbacks. Hence, the usage of antioxidants and oxidants in the treatment of leukemia is being explored and has been preliminarily applied. This article reviews the research progress of oxidative stress and leukemia. In addition, the application of antioxidants treatment in leukemia has been summarized.

简介：Theinteractionsofoxidativeradicals(Br2,HOetc.)withN-phosphoryldipeptidederivatives(NDM-TrpOMeandNDT-MetOMe)havebeeninvestigatedbyusingpulseradiolysisatdifferentpHvalues.IthasbeenfoundthatBr2andHOradicalsoxidizetheMet-siteandTrp-siteinthedipeptidederivativesviaformationofthethree-electron-bondedintermediateandindolylradicalsimultaneously.Thentheintramolecularelectrontransferalongthepeptidebackboneoccurs.Therateconstantsofelectrontransfer,k,havebeendeterminedandthereactionmechanismhasbeendeduced.

简介：Theoxidativesplittingprocessofcis-syn1,3-dimethyluracilcyclobutanedimer(DMUD)inaqueoussolutionwasinvestigatedusingpulseradiolysistechnique.TheresultsindicatedthatDMUDcanbesplittedinto1,3-dimethyluracil(DMU)byOHradicals(OH)andBr2radicalanions(Br2^-),butnotbyazideradicals(N3^).TheoxidativemechanismsthatanH-abstractedfromDMUDforOHoxidativesplittingandanelectrontransferfromDMUDtoBr2-,weresuggested.Relatedkineticparametersweredetermined.

简介：Athree-dimensionalgeometricmodeloftheoxidativecouplingofmethane(OCM)packed-bedreactorloadedwithNa2WO4-Mn/SiO2particulatecatalystwassetup,andanimprovedStanschkineticmodelwasestablishedtocalculatetheOCMreactionsusingthecomputationalfluiddynamicsmethodandFluentsoftware.Thesimulationconditionswerecompletelythesamewiththeexperimentalconditionsthatthevolumevelocityofthereactantwas80mL/minunderstandardstate,theratioofCH4/O2was3,thetemperatureandpressurewere800°Cand1atm,respectively.Thecontourofthecharacteristicsparametersinthecatalystbedwasanalyzed,suchasthespeciesmassfractions,temperature,theheatfluxonsidewallsurface,pressure,fluiddensityandvelocity.TheresultsshowedthatthecalculatedvaluesmatchedwellwiththeexperimentalvaluesontheconversionofCH4andtheselectivitytoproducts(C2H6,C2H4,CO2,CO)inthereactoroutletwithanerrorrangeof±2%.ThemassfractionsofCH4andO2decreasedfrom0.6and0.4inthecatalystbedinletto0.436and0.142intheoutlet,wherethemassfractionsofC2H6,C2H4,COandCO2were0.035,0.061,0.032and0.106,respectively.Duetotheexistenceoflaminarboundarylayer,thecontoursofeachcomponentbentupwardsinthevicinityoftheboundarylayer.ThisOCMreactionwasvolumeincreasereactionandthetotalmolesofproductsweregreaterthanthoseofreactants.Theflowfieldinthecatalystbedmaintainedconstanttemperatureandpressure.Thefluiddensitydecreasedgraduallyfrom2.28kg/m3intheinletofthecatalystbedto2.22kg/m3intheoutletofthecatalystbed,whilethevelocityincreasedfrom0.108m/sto0.115m/s.

简介：AbstractRespiratory health in the general population declines regardless of the presence of pulmonary diseases. Oxidative stress has been implicated as one of the mechanisms involved in respiratory dysfunction. This review was to evaluate studies that relate oxidative stress factors with pulmonary function among the general population without prior respiratory illnesses. The search yielded 54 citations. Twenty-one studies qualified for incorporation in this review. Owing to the heterogeneity of the review, studies were discussed based on identified oxidative stress factors responsible for pulmonary dysfunction. Oxidative stress biomarkers, including gene polymorphisms of nuclear factor erythroid 2-related factor 2, heme oxygenase 1, glutathione S transferase, superoxide dismutase, and lipid peroxidation products were involved in lung function decline. In addition, the antioxidant status of individuals in reference to dietary antioxidant intake and exposure to environmental pollutants affected oxidative stress and pulmonary function, as indicated by forced expired volume in one second, forced vital capacity, and forced expiratory flow at 25%-75%. This review indicated that oxidative stress is implicated in the gradual decline of lung function among the general population, and gene polymorphism along the antioxidant defense line and/or their interaction with air pollutants reduce lung function. Different polymorphic forms among individuals explain why the rate of lung function decline differs among people. Dietary antioxidants have respiratory health benefits in antioxidant gene polymorphic forms. Therefore, the genetic composition of an individual may be considered for monitoring and identifying people at risk of respiratory illnesses.

简介：Oxidativedesulfurization(ODS)ofAzericrudeoilwasstudiedundertheelectricfield.Bymeansoforthogonalexperiments,theoptimumconditionsfordesulfurizationofcrudeoilwereidentified,whiletheeffectofoxidativedesulfurizationundertheelectricfieldwascomparedwiththatofthesingleoxidativedesulfurizationmethod.Thetestresultsshowedthatthedesulfurizationreactiontakingplaceundertheelectricfieldwouldbebetterthanthetraditionalmethod.Thedesulfurizationrateofcrudeoilcouldreachupto77.06%at115℃atadistilledwateramountof10m%,adesulfurizerdosageof200μg/g,andademulsifierdosageof50μg/g.

简介：BACKGROUND:Ithasbeensuggestedthatmelatonin(MT)canprotectsecondaryneuronalinjury.However,theprotectiveeffectofMTonneuronalinjuryinischemia/reperfusionmodelsinvitrostillhasnotbeenproved.OBJECTIVE:ToinvestigatetheprotectiveeffectofMToncentralischemicinjuryofnervecellsandanalyzeitspossiblemechanism.DESIGN:Contrastobservationalstudy.SETTING:DepartmentofBiochemistryandMolecularBiology,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology.MATERIALS:Ratsaged7-8daysandweighing10-12gwereprovidedbyMedicalExperimentalAnimalCenter,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,MTwasprovidedbySigmaCompany,USA.METHODS:TheexperimentwascarriedoutintheLaboratoryofBiochemistryandMolecularBiology,TongjiHospital,HuazhongUniversityofScienceandTechnologyfromOctober2002toMarch2004.TheeffectsofMTontheneurodegenerationinducedbyoxygen-glucose-deprivation(OGD)weretestedinculturedratcerebellargranulecells.NeurondamagewasquantitativelyassessedbyTypanBlueexclusionandMTTassayatdifferenttimepointsafteroxygen-glucose-deprivation(90minutes).DNAgelelectrophoresisandacridineorangestainwereperformedtodeterminethenatureofcelldamage.Andfluorescencespectrophotometerwasusedforquantificationofintracellularmalondialdehyde(MDA)atvarioustimeintervals.MAINOUTCOMEMEASURES:Correlationbetweendegreesofneuronalinjuryandreperfusiontimes,apoptosis,andproductionofMDAincells.RESULTS:①Theneuroninjurywasaggravatedwithreperfusiontime.②TheprotectiveeffectofMTwastime-anddose-dependentwhenitsconcentrationwasnothigherthan10μmol/L.⑧WhenneuronswereexposedtoOGDfor90minutes.partofthecellsexhibitedtypicalfeaturesofapoptosis:internucleosomalDNAcondensationandDNAladderonagarosegelelectrophoresis.MTaddedtocellsrecoveringfromOGDexertedneuroprotectiveactionagainstOGD-inducedapoptosis.④InOGDexposedculture

简介：这研究的目的是决定包括伪-ketoacids(伪-ketoglutarate和pyruvate)，抗氧化剂的效果喂奶并且glutamate/malate联合，对老鼠精子上的氧化应力。我们的结果显示出那H2O2(250渭molL?1)导致的损坏例如损害活动性，腺苷triphosphate(ATP)弄空，精子蛋白质phosphorylation的抑制，减少的acrosome反应和减少的生存能力，能被精子的孵化显著地与伪-ketoglutarate阻止(4mmolL?1)或pyruvate(4mmolL?1)。没有在媒介的外长的H2O2，pyruvate的增加(4mmolL?1)显著地增加了superoxide阴离子(O2?在精子暂停的路)水平(P鈮?0.01)，而伪-ketoglutarate的增加(4mmolL?1)并且喂奶(4mmolL?1)有95kDa的尺寸的显著地提高的tyrosine-phosphorylated蛋白质(P鈮?0.04)。同时，伪-ketoglutarate，pyruvate，喂奶，在媒介补充的glutamate和malate能为精子活动性被用作重要精力来源和供应ATP。在结论，现在的结果证明伪-ketoacids能是为保护老鼠精子免受H2O2攻击的伤害的有效抗氧化剂并且能是有效部件改进Biggers，Whitten和Whittingham媒介的抗氧化剂能力。

简介：新聚合物，有氧化(PSFN)的poly-silicon-ferric凝结剂被把KMnO_4和稳定器M加到poly-silicon-ferric(PSF)生产凝结剂。PSFN的微性质与光显微镜，传播电子显微镜(TEM)，扫描的紫外/可见的吸收(UVA)，infrared(红外)分光计和oxidation-reductionpotential(ORP)被调查米分别地，与PSF的相比。由PSFN的凝结行为被PSF与那相比调查。结果证明PSF的微性质由于KMnO_4的增加极大地被改变了，并且在那里存在在PSFN独立的KMnO_4。一种四面体结构可以被Mn的thecomplexation有点象Si-O-Si契约的连接一样形成(也许在各种各样的原子价)与PSF。PSFN在更低的剂量比PSF有更低的混浊移动并且当剂量到达明确的数量时，完成一样，当由PSFN的移动ofUV_(254)在有大约17%的最大的差别的全部剂量范围上几乎由PSF比那高时。PSFN让更多的氧化以基本状况比那以酸的状况工作，并且为由PSF比那对待各种各样的水给申请的更强壮的能力。

简介：AIM:NitrativeandoxidativeDNAdamagesuchas8-nitroguanineand8-oxo-7,8-dihydro-2'-deoxyguanosine(8-oxodG)formationhasbeenimplicatedininitiationand/orpromotionofinflammation-mediatedcarcinogenesis.TheaimofthisstudyistoclarifywhethertheseDNAlesionsparticipateintheprogressionofintrahepaticcholangiocarcinoma.METHODS:Weinvestigatedtherelationoftheformationof8-nitroguanineand8-oxodGandtheexpressionofhypoxia-induciblefactor-1α(HIF-1α)withtumorinvasionin37patientswithintra-hepaticcholangiocarcinoma.RESULTS:Immunohistochemicalanalysesrevealedthat8-nitroguanineand8-oxodGformationoccurredtoamuchgreaterextentincanceroustissuesthaninnon-canceroustissues.HIF-1αcouldbedetectedincanceroustissuesinallpatients,suggestinglowoxygentensioninthetumors.HIF-1αexpressionwascorrelatedwithinduciblenitricoxidesynthase(iNOS)expression(r=0.369andP=0.025)and8-oxodGformation(r=0.398andP=0.015).DoubleimmunofluorescencestudyrevealedthatiNOSandHIF-1αco-localizedincanceroustissues.Notably,theformationof8-oxodGwascorrelatedsignificantlywithlymphaticinvasion(r=0.386andP=0.018).Moreover,8-nitroguanineand8-oxodGinnon-canceroustissueswereassociatedsignificantlywithneuralinvasion(P=0.042andP=0.026,respectively).TheseresultssuggestthatreciprocalactivationbetweenHIF-1αandiNOSmediatespersistentDNAdamage,whichinducestumorinvasivenessviamutations,resultinginpoorprognosis.CONCLUSION:Theformationof8-nitroguanineand8-oxodGplaysanimportantroleinmultiplestepsofgeneticchangesleadingtotumorprogression,includinginvasiveness.

简介：

简介：Oxidativestressiscloselyassociatedwithsecondarycelldeathinmanydisordersofthecentralnervoussystemincludingstroke,Parkinson’sdisease,Alzheimer’sdisease.Amongmanyaberrantoxidativestress-associatedproteins,DJ-1hasbeenassociatedwiththeoxidativestresscelldeathcascadeprimarilyinParkinson’sdisease.Althoughprincipallyexpressedinthecytoplasmandnucleus,DJ-1canbesecretedintotheserumunderpathologicalcondition.Recently,aclosepathologicalassociationbetweenDJ-1andoxidativestressinstrokehasbeenimplicated.Tothisend,weandothershavedemonstratedtheimportantroleofmitochondriainneuroprotectionforstrokebydemonstratingthatthetranslocationofDJ-1inthemitochondriacouldpotentiallymitigatemitochondrialinjury.Here,wediscussourrecentfindingstestingthehypothesisthatDJ-1notonlyfunctionsasaformofintracellularprotectionfromoxidativestress,butthatitalsoutilizesparacrineand/orautocrinecuesinordertoaccomplishextracellularsignalingbetweenneighboringneuronalcells,resultinginneuroprotection.ThisarticlehighlightsrecentevidencesupportingthestatusofDJ-1askeyanti-oxidativestresstherapeutictargetforstroke.

简介：

简介：AbstractObjective:Clinically, low-dose aspirin and progesterone are frequently used to prevent pregnancy loss. We investigated the effect of these drugs on the biological behavior of human extravillous trophoblasts in vitro.Methods:HTR-8/SVneo cells were cultured in vitro and treated with different concentrations of aspirin and progesterone. The proliferation, invasion, and apoptosis of HTR-8/SVneo cells were assessed using a cell counting Kit-8 assay, Matrigel Transwell assay, and Hoechst staining, respectively. Reverse transcriptase polymerase chain reaction was used to verify the expression of related genes. Reactive oxygen species (ROS) levels were detected using the 2,7-dichlorofluorescin diacetate assay.Results:Low-dose aspirin alone, progesterone alone, or aspirin plus progesterone upregulated the proliferation and invasion and decreased the apoptosis of HTR-8/SVneo cells. Moreover, the expression of marker of proliferation Ki-67 (MKI67), matrix metalloproteinases 2 (MMP2), and MMP9 was increased. In addition, low-dose aspirin plus progesterone exerted stronger anti-apoptosis effects than low-dose aspirin and progesterone alone. Interestingly, aspirin upregulated the expression of progesterone receptor (PGR). Treatment with hydrogen peroxide (H2O2) promoted ROS production in HTR-8/SVneo cells; however, low-dose aspirin plus progesterone significantly restricted H2O2-mediated ROS production and apoptosis in HTR-8/SVneo cells.Conclusions:These data suggest that low-dose aspirin and progesterone promote proliferation and invasion and cooperatively reduce oxidative stress and apoptosis in trophoblasts in vitro. These results may provide an experimental basis for the combined application of aspirin and progesterone to prevent unexplained recurrent spontaneous miscarriage, especially in patients with trophoblast dysfunction.

简介：Biodiesel(fattyacidalkylesters),analternatetofossilfuel,hasthetendencyofautoxidationandhencerequiresantioxidantsforlongtermstorage.TheinfluenceofsyntheticandnaturalantioxidantsontheoxidativestabilitywasanalysedforfreshFAMEs(fattyacidmethylesters)obtainedfromheveabrasiliensisat140C.HigheractivitywasobservedforsyntheticantioxidantsfollowingtheorderofGA>BHT>DTBP>Q>GT-M>PH-M>GT-C,whereastheoxidativestabilityofstoredFAMEssamplesmeasuredat110Crevealsanearlyinversetrend.StoragestabilitywastestedfortheFAMEsobtainedfromheveabrasiliensisstoredat30C,afteradditionofsyntheticandnaturalantioxidants—butylatedhydroxytoluene(BHT),2,6-di-tert-butylphenol(DTBP),quercetin(Q),gallicacid(GA),methanolextractsfromgreentea(GTM),pomegranatehull(PH-M),andchloroformextractofgreentea(GT-C).Antioxidantactivitiesabove1500ppmwasintheorderofDTBP>BHT>GA>GT-C>GT-M>Q>PH-M.SyntheticantioxidantshavebeenfoundmoreefficienttoimprovethestoragestabilityofFAMEsobtainedfromheveabrasiliensis.DTBPinparticularhasthehighestprotectionfactor.

简介：TheWisonEngineering(China)Company,Ltd.hasannouncedthatithassuccessfullydevelopedanewtechnologyformanufacturingbutadienethroughoxidativedehy-drogenationofbutylenefollowingthecompletionofPDPfora75kt/a

简介：目的methotrexate(MTX)的最重要的副作用是mucositis。这研究的目的是在收到methotrexate的老鼠在肠的损坏和氧化应力上评估沉金摘录的效果。方法实验在划分成六个组的男Wistar白化体老鼠上被执行。首先组织收到的正常盐口头上地，第二个组收到了沉金摘录(100mg瑡潩?????????????????假吗？？

简介：Byusingthebimodalmesoporoussilica(BMMS)asthecarrierandbutyltitanateasthetitaniumsource,theTiO2/BMMScatalystwasprepared.ThesampleswerecharacterizedbyXRD,XRF,N2adsorptionanddesorption,FTIR,UVvis,SEM,EDS,andTEMtechniques.ThetestresultsshowedthatTiO2wasamorphous,theTiO2/BMMScatalysthadanorderedbimodalmesoporousstructure,andthechemicalinteractionexistedbetweenBMMSandTiO2.SincetheTiO2/BMMShadalowerbandgap,itsphotocatalyticactivitywasbetterthanTiO2.UnderUVirradiationaone-potPODSsystemwassetup,usingTiO2/BMMSasthecatalyst,H2O2astheoxidant,andmethanolasthesolvent.TheTiO2/BMMScatalystshowedbetterphotocatalyticactivitythanthemono-modalmesoporousTiO2/SBA-15catalyst,andthedesulfurizationrateofdibenzothiophene(DBT)overTiO2/BMMScatalystcouldreach99.2%.TheTiO2/BMMScatalystalsohadsogoodstabilitythatthedesulfurizationrateofDBTdidnotdropapparentlyafter8cyclesofreusing,andcouldstillbecloseto90%.

简介：Inthiswork,theremovalofthiophenefromsimulatedoilhasbeenstudiedbyusingtheadsorption,extractionandoxidation/adsorptionmethods,respectively.Intheadsorptivedesulfurizationprocess,differentcommercialadsorbentswereusedtoeliminatethiopheneatambientpressureandmildtemperature,andtheresultsshowedthatcarbonpowderhadthebestadsorptionability.Intheextractivedesulfurizationprocess,thebestdesulfurizationresultwasobtainedwhenDMFisused.Intheoxidative/adsorptivedesulfurizationprocedureusingsynthesizedpotassiumferrateastheoxidantandphosphomolybdicacidsolutionasthecatalyst,thiophenewasoxidizedandremovedfromhydrocarbonsincombinationwithactivecarbonadsorption,andtheresidualsulfurcontentofsimulatedoilcouldbereducedto15.3mg/Lfromtheoriginallevelof200mg/L,withthedesulfurizationratereaching92.3%.

简介：Zygosaccharomycesrouxiiisasalt-tolerantyeastspeciescapableofremovingcadmium(Cd)pollutantfromaqueoussolution.Presently,thephysiologicalcharacteristicsofZ.rouxiiunderthestressofsodiumchloride(NaCl)andCdarepoorlyunderstood.ThisstudyinvestigatedtheeffectsofNaClandCdonthegrowth,oxidativestressandantioxidantenzymeactivitiesofZ.rouxiiafterstresstreatmentfor24h.ResultsshowedthatNaClorCdalonenegativelyaffectedthegrowthofZ.rouxii,butthegrowth-inhibitingeffectofCdonZ.rouxiiwasreducedinthepresenceofNaCl.FlowcytometryassayshowedthatunderCdstress,NaClsignificantlyreducedtheproductionofreactiveoxygenspecies(ROS)andcelldeathofZ.rouxiicomparedwiththoseintheabsenceofNaCl.Theactivitiesofsuperoxidedismutase(SOD),catalase(CAT),andperoxidase(POD)ofZ.rouxiiweresignificantlyenhancedby2%–6%NaCl,whichlikelycontributedtothehighsalttoleranceofZ.rouxii.ThePODactivitywasinhibitedby20mgL-1CdwhiletheSODandCATactivitieswereenhancedby8mgL-1Cdandinhibitedby20mgL-1or50mgL-1Cd.Theinhibitoryeffectofhigh-levelCdontheantioxidantenzymeactivitiesofZ.rouxiiwascounteractedbythecombineduseofNaCl,especiallyat6%.ThisprobablyaccountedforthedecreaseinCd-inducedROSproductionandcelldeathofZ.rouxiiafterincubationwithNaClandCd.OurworkprovidedphysiologicalcluesastotheuseofZ.rouxiiasabiosorbentforCdremovalfromseawaterandliquidhighlysaltyfood.