简介：

简介：TherootsofPaeoniaelactifloraPall.arewidelyconsumedascrudedrugsinAsiancountriesduetotheirremarkablebeneficialhealtheffects.Thepresentresearchwasundertakentoilluminatethedynamicchangesinmetabolitesandenzymesandfacilitateselectionoftheharvestingtimewhentheherbcanprovideoptimumhealthbenefits.P.lactiflorarootswereanalyzedat12stagesofgrowthformonoterpenoidglycosides,phenols,nucleosides,nucleobases,aminoacids,andpolysaccharidesbyhigh-performanceliquidchromatographywithphotodiodearraydetector,ultra-highpressureliquidchromatographycoupledwithtandemmassspectrometry,andUVspectrophotometry.Theenzymeactivitiesofplantβ-glucosidasesandesterasesweredeterminedbyUVmethods.ThetotalcontentofmonoterpenoidglycosidesandphenolspeakedinDecember.Fornucleosidesandnucleobases,thehighestcontentappearedinApril.ThemaximumphasicaccumulationofthetotalaminoacidstookplaceinMarch,andthecontentoftotalpolysaccharidesreachedapeakvalueinSeptember.December,April,andMarchwereselectedastheappropriateharvestingtimesforproducingnaturalmedicinalorhealthfoodproducts.Plantβ-glucosidasesandesterasesshowedthehighestactivityinDecemberandMay,respectively.Whentheactivityofβ-glucosidaseincreased,esteraseactivitydecreased,whilethecontentsofoxypaeonifloraandpaeoniflorinincreased.Whenesteraseactivityincreased,thecontentsofbenzoylpaeoniflorin,paeoniflorin,andgallicaciddecreased.Inconclusion,theresultsfromthepresentstudywouldbeusefulindeterminationofthesuitabletimeforharvestingP.lactiflorarootsformedicinalpurposes.