简介：AneuploidyembryosofPinctadamartensiiDunkerareproducedduringtetraploidinductionbyinhibitingthefirstpolarbodyineggsfromtriploidfertilizedwithhaploidspermswithcytochalasinBtreatment.Chromosomeanalysisrevealsthatthereare88.18±6.79%aneuploidyembryos,and28.70%aneuploidsinpearloystersofone-yearageTheseaneuploidshavefivechromosomalconditions,suchas2n+1(29),2n+2(30),3n-2(40),3n-1(41)and3n+1(43).Resultsofgrowthmeasurementshowthatthereisnosignificantdifferencebetweenaneuploids(asagroup)anddiploidsinbodysizeandweight(p＞0.10),buttheaneuploideisobviouslydifferentfromtriploid(p＜0.01).Themeanbodysizeandweightofaneuploidsindiploidcondition(2n±1and2n±2)aresignificantlysmallerthanthoseofdiploids(p＜0.01),butaneuploidswithintriploidcondition(3n±1and3n±2)arenotsmallerthandiploidsinbodysizeandweight(p＞0.1).ThisstudyindicatesPinctadamartensiiDunkercouldtolerateaneuploidyby7～14%ofthehaploidgenome,andthataneuploidsofthisspeciesareviableundercertainconditions.

简介：Inthisstudy,weexaminedtheeffectofelevatedtemperatureontheexpressionpatternsofgenes,i.e.,nacrein,irr,n16,n19,andhsp70inthepearloysterPinctadafucata.Theexperimentwascarriedoutat4temperatures,i.e.,20℃(ambient,control),24,28℃,and32℃.TheexpressionlevelsoftargetgenesinP.fucatawereassayedat0,6,24,48,and96hviareal-timepolymerasechainreaction.Resultsshowedthattheexpressionlevelsofnacreinandirrhadnosignificantvariationsamongdifferenttimepointsbelow28℃,butsignificantlyincreasedovertimeat32℃.Theexpressionlevelsofn16andn19didnotchangemarkedlyat20℃.Theformerincreasedsignificantlyat6hand24hwhilethelattersubstantiallydecreasedduring6–96hat24,28and32℃.Amongdifferenttemperatures,thelevelofn16wassignificantlylowerat20℃thanatothertemperaturesduring6–96h,andthelevelofn19significantlyvariedamongdifferenttemperaturesat48hand96h.Theexpressionlevelofhsp70wassignificantlyhigherat32℃thanat20,24and28℃at24h.TheseresultsdemonstratedthatelevatedtemperatureimpactedthephysiologicalprocessesofP.fucataandpotentiallyinfluenceditsadaptabilitytothermalstress.