简介：

简介：

简介：摘要目的探讨血清miRNA-145（miR-145）、miRNA-21（miR-21）和miRNA-197（miR-197）对非小细胞肺癌（NSCLC）的诊断价值。方法回顾性分析2019年1月至2020年6月四川省泸州市中医医院收治的65例NSCLC患者的临床资料，以60名健康体检者为健康对照组。采用实时荧光定量聚合酶链反应（qRT-PCR）检测所有受试者血清miR-145、miR-21和miR-197的相对表达量，分析其与NSCLC患者临床病理特征的关系。采用受试者工作特征（ROC）曲线分析miR-145、miR-21和miR-197单独及联合诊断NSCLC的效能。结果NSCLC组miR-145相对表达量低于健康对照组（1.05±0.40比1.38±0.44，t＝-4.326，P<0.01），miR-21、miR-197相对表达量均高于健康对照组（2.37±0.89比0.83±0.25，4.42±0.75比1.10±0.33，均P<0.01）。NSCLC组中，TNM分期Ⅲ～Ⅳ期患者血清miR-21和miR-197相对表达量均高于Ⅰ～Ⅱ期患者（均P<0.01），腺癌患者miR-145相对表达量高于鳞状细胞癌患者（P<0.01），淋巴结转移患者miR-21相对表达量高于无淋巴结转移患者（P<0.01）。miR-145、miR-21、miR-197单独诊断NSCLC时，曲线下面积（AUC）分别为0.842（95% CI 0.795～0.907）、0.868（95% CI 0.812～0.938）、0.857（95% CI 0.801～0.913），诊断最佳截断值分别为1.14、2.03、2.98；采用最佳截断值进行诊断时，单独检测灵敏度最高的是miR-145（83.76%），特异度最高的是miR-21（87.63%）；三者联合检测时AUC为0.939（95% CI 0.904～0.965），诊断灵敏度为91.58%，特异度为79.86%。结论miR-145、miR-21、miR-197相对表达量与NSCLC患者病理类型、TNM分期、淋巴结转移相关，可能成为辅助诊断NSCLC的标志物，三者联合检测可提高诊断效能和灵敏性。

简介：ThemicrostructureofsynthesizedAT(A-luminumtitanate)hasbeenimprovedefficient-ly,AndthemicrocrackofATformedduringcoolingandabnormalgraingrowthhavebeenlimitd.Therefore,thesinterabilityandmechanicalpropertiesofATmaterialhavebeenenhanced.

简介：

简介：

简介：摘要目的探讨外周血miRNA-146a、miRNA-155和miRNA-186在重症肺炎患者中的表达及临床意义。方法选择宁波市医疗中心李惠利医院东部院区2019年2月至2021年2月收治的重症肺炎患者79例为研究对象，按照入院28 d预后情况，分为存活组（58例）与死亡组（21例）；另选择同时段健康体检者60例为对照组。采集研究对象外周血标本，分离血清，采用实时荧光定量PCR法（qRT-PCR）测定外周血miRNA-146a、miRNA-155和miRNA-186表达。结果重症肺炎组急性生理学和慢性健康状态Ⅱ（APACHE Ⅱ）评分［（18.54±2.83）分］高于对照组［（3.18±0.57）分］（t=41.37，P < 0.05）。重症肺炎组外周血miRNA-146a相对表达量（0.32±0.07）低于对照组（1.08±0.21），而miRNA-155相对表达量（2.54±0.46）和miRNA-186相对表达量（3.16±0.38）均高于对照组［（0.42±0.09）、（0.89±0.17）］（t=30.07、35.16、43.08，均P < 0.001）。死亡组外周血miRNA-146a相对表达量（0.25±0.68）低于存活组（0.59±0.12），而miRNA-155相对表达量（3.97±0.78）和miRNA-186相对表达量（5.23±0.86）均高于存活组［（0.89±0.21）、（1.52±0.23）］（t=19.11、27.69、30.29，均P < 0.001）。APACHE Ⅱ评分与miRNA-146a呈线性负相关（r=-0.75，P=0.015），而与miRNA-155和miRNA-186呈线性正相关（r=0.82、0.70，P=0.002、0.021）。重症肺炎诊断中，miRNA-146a灵敏度为72.34%，特异度为62.50%；miRNA-155灵敏度为75.00%，特异度为62.96%；miRNA-186灵敏度为66.67%，特异度为48.65%。结论外周血miRNA-146a在重症肺炎中低表达，而miRNA-155和miRNA-186在重症肺炎中高表达，且miRNA-146a、miRNA-155和miRNA-186与患者预后密切相关。

简介：MicroRNA（miRNA）是一类广泛存在于人、动植物和病毒的内源长度约为21～25个核苷酸的非编码小RNA，且在不同组织、不同阶段存在特异性表达，在真核生物发育和基因表达中通过与靶mRNA形成完全或不完全互补配对从而扮演着重要角色。参与动物体发育、细胞增殖与死亡、细胞分化等各种过程。文章综述了miRNA对家禽发育生长、脂质代谢、疾病控制等方面的研究进展，为进一步研究miRNA在家禽中功能提供理论依据和事实参考。

简介：MicroRNAs(miRNAs)areaclassofshort,endogenously-initiatednon-codingRNAsthatpost-transcriptionallycontrolgeneexpressionviaeithertranslationalrepressionormRNAdegradation.ItisbecomingevidentthatmiRNAsareplayingsignificantrolesinregulatorymechanismsoperatinginvariousorganisms,includingdevelopmentaltimingandhost-pathogeninteractionsaswellascelldifferentiation,proliferation,apoptosisandtumorigenesis.Likewise,asaregulatoryelement,miRNAitselfiscoordinativelymodulatedbymultifariouseffectorswhencarryingoutbasicfunctions,suchasSNP,miRNAediting,methylationandcircadianclock.Thismini-reviewsummarizedthecurrentunderstandingofinteractionsbetweenmiRNAsandtheirtargets,includingrecentadvancementsindecipheringtheregulatorymechanismsthatcontrolthebiogenesisandfunctionalityofmiRNAsinvariouscellularprocesses.

简介：摘要：miRNA在基因调控方面起着重要作用，其异常表达和特异性表达提示其可成为诊断或预后的生物标志物。因此准确测定其表达水平对于其应用十分关键。但是由于miRNA序列短，同源序列相似度高，含量低，缺乏能够使其选择性扩增的共同特征，使得检测miRNA十分具有挑战性。本文对近年来miRNA的检测方法进行了概述，包括传统方法及其改进进展，以及一些新的检测方法，并对这些方法的优缺点进行了总结。

简介：SmallinterferingRNA(siRNA)andmicroRNA(miRNA)aresmallRNAsof18-25nucleotides(nt)inlengththatplayimportantrolesinregulatinggeneexpression.TheyareincorporatedintoanRNA-inducedsilencingcomplex(RISC)andserveasguidesforsilencingtheircorrespondingtargetmRNAsbasedoncomplementarybase-pairing.ThepromiseofgenesilencinghasledmanyresearcherstoconsidersiRNAasananti-viraltool.However,inlong-termsettings,manyvirusesappeartoescapefromthistherapeuticalstrategy.Anexampleofthismaybeseeninthecaseofhumanimmunodeficiencyvirustype-1(HIV-1)whichisabletoevadeRNAsilencingbyeithermutatingthesiRNAtargetedsequenceorbyencodingforapartialsuppressorofRNAi(RNAinterference).Ontheotherhand,becausemiRNAtargetingdoesnotrequireabsolutecomplementarityofbase-pairing,mutationalescapebyvirusesfrommiRNAspecifiedsilencingmaybemoredifficulttoachieve.Inthisreview,wediscussstratagemsusedbyvariousvirusestoavoidthecells'antiviralsi/mi-RNAdefensesandnotionsofhowvirusesmightcontrolandregulatehostcellgenesbyencodingviralmiRNAs(vmiRNAs).

简介：

简介：

简介：独角兽是西方神话传说中的一种虚构生物。形如白马，额前有一个螺旋角，代表稀有、高贵、高傲和纯洁。如今,这个西方世界的神兽，已经成为最具创新能力的成功食业的图腾.它能够在极短时间内，凭借自己独有的基因，率先深入挖掘新经济中的巨大机遇.

简介：Theinfluenceofinitialstrainstateonthedynamicresponseofanendbearingpileembeddedinisotropicsaturatedsoilisinvestigatedthroughthelinearizedtheoryofsmallelasticperturbationsuperposedonlargelystressedbodies.Thegoverningequationsforsoil,basedonBiot’sporoelasticitytheory,arederivedinthecylindricalcoordinates,andthepileismodeledbyusingtheone-dimensionalelastictheory.Theanalyticalsolutionsofpileimpedance,frequencyresponseofbothtwistangleandtimehistoryofvelocityresponseareobtainedbyusingofseparationofvariablestechnique.Finally,aparametricstudyoftheinfluenceofinitialstrainsonthetorsionalimpedance,twistangle,andvelocityresponseatthetopofthepileiscarriedout.

简介：

简介：无

简介：Asinreadingteaching,schematheoryhaslongbeentakingadominantpart.Accordingtothistheory,Englishteacherscanhelpthestudentstocalluptheirpriorknowledgebyplacinganemphasisonamoreefficientuseofpre-readingactivities.Oneoftheeffectiveactivitiesistomakegooduseofthepre-readingexercisesinthetextbook.Thispaperintendstoanalyzetheresultsoftwobriefinvestigations.Onewasaboutthepre-readingexercisesintheSEFC(NewEdition2010)Book1.Theotherwascar-riedoutamongthefirstyearstudentsfromNanhaiExperimentalHighSchoolofSouthChinaNormalUniversity.Englishteachersinhighschoolthuscantrainthestudentstomakelinksbetweenthetextandwhattheyalreadyknowaccordingtotheirinterests.

简介：Pre-paintedsteelisoneofthemostimportantstructuralmaterialofthe20thcenturywellknownforitsexcellentcorrosionresistanceandwiderangingapplications.Atypicalpre-paintedsteelusuallyconsistsofalayerofmetalcoatingsystem,preferablyzincorzincalloycoatingandacombinationoflayersofinorganic-organiccoatingsusuallyreferredtoaspaintsystem.Thecorrosionresistanceofthemetalcoatingaswellasthepaintsystemmayvaryconsiderablybasedontheircompositionandtheenvironment.Foroptimalcorrosionperformanceofpre-paintedsteel,ajudiciousselectionofbothmetalandinorganic-organiccoatingsystemsareessential.Thispaperreviewsdifferenttypesofpossiblecorrosionissuesinpre-paintedsteelsandmethodstooptimisetheirperformance.

简介：ThereisagoodcorrelationbetweenanomalousSchumannresonanceandseismicactivities.Pre-earthquakeanomalousSchumannresonanceisanimportantmanifestationofLAIC(Lithosphere-Atmosphere-IonosphereCoupling),whichisprobablyassociatedwithseismicionosphericdisturbance.TheobservationsystemparametersoftheYunnanSchumannresonancemonitoringstationsandtheresultsofstatisticalandseismiccasesanalysisareintroducedinthispaper.ThreedaysbeforetheMarch10,2011MS5.8Yingjiangearthquake,anomalousSchumannresonanceintheBycomponent(E-W)wasobservedbyYongshengstation,andtwodaysbeforetheLincangMS4.0earthquakeofJanuary23,2011,anomalousSchumannresonanceintheBycomponentwasdetectedbythesamestation.SpectrumanalysisresultsontheBycomponentobservationsofTonghaistationshowthatstrongdisturbanceswereobservedinspectraldensitycorrespondingtothefirstthreeSchumannresonantfrequenciesontheverydayoftheMS4.2HongheearthquakeofMay22,2011.ResearchshowsthatSchumannresonancemonitoringisafeasiblemethodforseismic-electromagneticprecursorsmonitoring.