简介：Objective:Stromalinteractionmolecule1(STIM1)overexpressionhasbeenreportedtoplayanimportantroleinprogressionofseveralcancers.However,themechanismofSTIM1overexpressionanditsrelationshipwithhypoxiainpancreaticductaladenocarcinoma(PDAC)remainsunclear.Methods:STIM1andHIF-1αexpressionwastestedusingimmunohistochemistryintissuemicroarray(TMA)includingpancreaticcancerandmatchednormalpancreatictissues,andtheirrelationshipswithclinicopathologicalparameterswerestatisticallyanalyzed.q-PCR,Westernblot,ChIP,andluciferaseassaywereemployedto030analyzetranscriptionalregulationbetweenHIF-1αandSTIM1inpancreaticcancerPANC-1cells.Results:BothSTIM1andHIF-1αshowedhigherpositiveratesandup-regulatedexpressionincancertissuescomparedtothatofnormaltissues(P<0.05).TheKaplan–MeiermethodrevealedthathigherHIF-1αandSTIM1expressionlevelsweresignificantlycorrelatedwithdecreaseddisease-freesurvival(P=0.025andP=0.029,respectively).TheexpressionofHIF-1αshowedasignificantpositivecorrelationwiththatofSTIM1incancertissues(rs=0.3343,P=0.0011)andpancreaticcancercelllines.Furthermore,ChIPandluciferaseassaysconfirmedthatHIF-1αboundtotheSTIM1promoterandregulateditsexpressioninPANC-1cells.Conclusions:Inhypoxiamicroenvironment,up-regulatedexpressionofSTIM1mediatedbyHIF-1αpromotesPDACprogression.HIF-1αandSTIM1arepotentialprognosticmarkersand/ortherapeutictargetsforPDACtreatment.

简介：EffectsoftheorientationandapexangleonthesettlingvelocityofconicalparticlesinBinghamplas-ticfluidsarenumericallyinvestigatedoveraReynoldsnumberrangeof1≤Re≤100,Binghamnumberrangeof0≤Bn≤100,andconeanglerangeof20≤α≤150°.Governingequations(ofcontinuityandmomentum)aresolvednumericallyusingthefiniteelementmethodtoobtainvelocityandpressurefieldsthatarepostprocessedtoobtainvaluesofthedragcoefficien.Futthermore,theeffectofconeori-entationontheflowfieldisvisualizedandexploredintermsofstreamlinecontoursandthemorphologyofyielded/unyieldedregions.Finally,theobtainedvaluesofdragarecorrelatedviaasimplepredictiveexpressionintermsofthemodifiedReynoldsnumber.Theeffectoftheconeangleondragisfoundtobemoderatebecausethedragforceactingonthelateralsurfaceisasmallfractionoftheoveralldrag.

简介：Immisciblefluidflowisfrequentlyfoundinresourcerecoveryorsoilremediation.Theflowpatternintheporousmediaisaffectedbythewettabilityofporesurface.Inthisstudy,quartzsubstratesandmicrofluidicchipsaretreatedbysilicananoparticles(SNP)andtriethoxy(octyl)silane(TES)tofabricatethewater-wetandoil-wetsurfaces.Thewettabilityofthetreated-surfaceismeasuredintermofcontactangleforseveralcombinationsofsurroundingfluidandaliquiddroplet.Theeffectofthewettabilityonthefluidflowpatternisexploredbyinjectingoilandwateralternatelyintothesurface-treatedmicrofluidicchips.TheresultsrevealthattheSNP-coatedquartzsubstrateshowsstrongwater-wetpropertyandtheTEScoatingmakeswater-repellent/oil-wetsurfaces.Inaddition,itisfoundthatthemaximumandminimumoilandwatersaturationduringalternateinjectionofoilandwaterdependsonthesurfacewettabilityofthemicrofluidicchips.Thecharacteristicsofthepore-scalefluidflowpatternarealsodescribed.

简介：Theharmonysearchalgorithmisappliedtooptimumdesignsofafunctionallygraded(FG)-carbonnanotubes(CNTs)-reinforcedpipesconveyingfluidsubjectedtomovingload.ThestructureismodelledbyReddycylindricalshelltheoryandthefinalequationsareobtainedbyprincipalofHamilton.Basedondifferentialquadraturemethod(DQM),thedynamicdisplacementofsystemisderived.

简介：Aone-dimensional(1D)fluidsimulationofdualfrequencydischargeinheliumgasatatmosphericpressureiscarriedouttoinvestigatetheroleofthesecondaryelectronemissiononthesurfacesoftheelectrodes.Inthesimulation,electrons,ionsofHe+andHe2+,metastableatomsofHe*andmetastablemoleculesofHe*2areincluded.Itisfoundthatthesecondaryelectronemissioncoefficientsignificantlyinfluencesplasmadensityandelectricfieldaswellaselectronheatingmechanismsandionizationrate.TheparticledensitiesincreasewithincreasingSEEcoefficientfrom0to0.3aswellasthesheath’selectricfieldandelectronsource.Moreover,theSEEcoefficientalsoinfluencestheelectronheatingmechanismandelectronpowerdissipationintheplasmaandbothofthemincreasewithincreasingSEEcoefficientwithintherangefrom0to0.3asaresultofincreasingofelectrondensity.

简介：摘要 义 184-斜 7井是位于济阳坳陷沾化凹陷渤南洼陷中部断阶带的一口注气井，该井面临着上部地层造浆严重，东营底部、沙河街易垮塌，沙四段地层压力系数高且密度窗口窄的难题。在经过对各地质层位深入研究并和邻井对比分析后，制定了合理的钻井液技术措施，该井于 2018年 11月 6日 6:00钻至井深 4133m三开完钻。钻进过程中二开转换泥浆体系是关键，三开钻井液流变性调整是重点，并且高密度钻井液性能维护不同于常规钻井液，助剂配伍性要好，而且加量要及时、均匀而充足，保证其有效含量，形成优质泥饼，从而降低失水和摩阻，确保井壁稳定、防止复杂事故的发生。

简介：Thepulsed-sprayfluid-bedgranulation(PSFBG)processwasinvestigatedandoptimizedusingdefinitivescreeningdesign,arecentlyproposednewclassofthree-leveldesignofexperimentmethod.Suchadesignenabledquadraticmodelstobeestablishedthatdescribedtheeffectofsixinputprocessparameters-inletairtemperature,inletairhumidity,bindersprayrate,atomizationpressure,pulseperiod,andpulsewidth-onthegranulequalityinaPSFBGprocess.Mathematicalmodelsofthemeanparticlesize,relativesizedistributionwidth,productionyield,andporosityweredevelopedtoquantifytherelationshipsbetweentheinfluencingfactorsandcriticalqualityattributes.Onthebasisofconstraintsonthedesiredgranuleproperties,adesignspaceforPSFBGwasdeterminedandrangesoftheoperatingparametersweredefined.Anacceptabledegreeofpredictionwasconfirmedbyvalidationexperiments,demonstratingthereliabilityandeffectivenessofusingdefinitivescreeningdesigntostudythePSFBGprocess.Thismethodcanacceleratescreeningandoptimizationofthisprocesswithinalargemultidimensionaldesignspace.