简介：VegetativecellsofPorphyrayezoensisareisolatedwithseasnailenzymeandculturedonthesolidifiedagarmedium.Theresultsofexperimentsshowthattheisolatedcellscansurvive,divideandregeneratewellonthemediumsolidifiedwithagar.Thefirstdivisiononthesolidmediumstartsafter7days'culture,4dayslaterthantheliquidculture.Thesurvivalrateofisolatedcellsis71.3%onthesolidmedium,lowerthanthe86.2%ofthatinseawater.Thalli,thalloids,conchocelis,spermatangiaandmulticellularmassesaredevelopedonthesolid/mediuminthefirstmonth,slowlybutnormally.Spermatangiasacsdisappearwithin4weeks.Withoutaddingnutrientliquidontothesurfaceofsolidmediumorinjectingseawaterundertheagarlayerinordertokeepmoisture,thethalliandcellgroupsreleasemonosporestoformnewthalliinsteadofenlargingtheirareasafter5weeks'culturing.Somemonosporesregeneratenewthalli.Othermonosporeslosetheirpigmentsandminimizetheirvolumeanddividequicklytoformlightpinkcalli.After16weeks,numerouscallicanbeseenonthesolidmediumandafter24weeks'culturing,almostonlycalliandconchoceliscanbeseen.Ifthecalliareimmersedinseawater,themonosporesarereleasedandmaydevelopintoyoungthallus.

简介：ThenewspreadingconchosporesotPorphyrayezoensis,P.haitanensisandP.katadaivar.hemiphyllaareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthat(1)nomutantshavebeeninvestigatedinallcontrolgroup.(2)TheconchosporesofallthethreespeciesofPorphyraareeasytobeinducedbyNGfortheyareinthestageofbreeding,Inthelowestdose(10μg/mL)ofthisexperiment,themutantrateofP.yezoensisisupto57.9%,thatofP.haitanensis30.7%andthatofP.katadaivar.hemiphylla51.7%.(3)Themutantratecannotincreaseobviouslybyincreasingthereagentconcentrationorprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofthreespeciesofPorphyraaremuchsimilar,andthemutantrateisnear50%.TheoptimuminductionconcentrationofNGis10μg/mL,andtheoptimuminductiontimeis30minutes.

简介：TheyoungthalliofPorphyrayezoensisandP.haitanensisareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthatnomutantshavebeeninvestigatedinallcontrolgroups,whileintheinducedgroups,itiseasytofindmutantcellsormutantcellmassesinthethallus.ThepigmentationmutantsmaybetheresultofNGinducement.Themutantrateincreasesobviouslywiththemutagenconcentrationorbyprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofPorphyrayezoensisareraisedfrom11.2%to28.7%,andthoseofP.haitanensisfrom10.1%to20.2%.Accordingtoourexperiments,theamountofmutantcellsinthesameareadoesnotincreasewiththeinductionintensityTheamountofmutantcellsineverygroupofinducementofbothspeciesofPorphyrachangesverylittlewiththefixedfieldscheckmethod.Thelowestdose(25μg/mL)ofthisexperimenthasahighmutantrate.Themutantrateshowsthepercentageofmutantcellsinallthesurvivedcells,whiletheamountofmutantcellsreflectstheeffectofinducement.ThustheoptimuminductionconcentrationofNGis25μg/mL,andtheoptimuminductiontimeis30minutes.

简介：

简介：PhycoerythrinandphycocyaninwerepurifiedfromPorphyrayezoensisUedawiththeirbioactivitydeterminedinthisstudy.Continuousprecipitationwithammoniumsulfateatdifferentconcentrations(10%,20%,40%and50%)increasedthepurity(A564:A280)ofphycoerythrinto1.49,3.92foldoftherawextract(0.38)andthepurity(A615:A280)ofphycocyaninto0.70,3.33foldoftherawextract(0.21).Twomoretimesofchromatographywithhydroxylapatitesfinallymadethepurityofphycoerythrinandphycocyaninreach5.50,14.47foldoftherawextract,and5.10,24.29foldoftherawextract,respectviely.Theyieldofhighpurityphycoerythrinandphycocyaninwere0.21%and0.09%ofdriedP.yezoensisblade,respectively.Thephotodynamiccytotoxicexperimentshowedthatbothphycoerythrinandphycocyanininhibitedthegrowthoflivertumorcellssignificantly.Itwasfoundthat250mgL-1purifiedphycoerythrinandphycocyanininhibitedthegrowthofhepatocellularcarcinomacells24hafterlaser-irradiationby80%and59%,respectively,and100mgL-1purifiedphycoerythrinandphycocyanininducedtheapoptosisof31.54%and32.54%ofthecells,respectively,8hafterphotodynamictherapy.OuefindingsdemonstratedthatP.yezoensiscanserveasphotosensitizer(phycoerythrinandphycocyanin)producer.

简介：表示顺序标注的Porphyrayezoensis(EST)的genomic资源被利用识别简单顺序重复(SSR)，或微卫星。这个方法拿了为基因身份的建立或为高多型性的获得使用EST和微卫星的优点。当基因标记whenmicrosatellites被附在基因，微卫星能被使用。由生物信息学分析揭示了，1162从21954颗ESTscontained微卫星和聚合分析显示984这些EST掉进112contigs，当另外的178EST是单条时。290唯一的包含SSR基因的一个总数被识别。AACSSR是微卫星的最人口多的类型。在所有微卫星之中占优势的GC富有的microsatelliteswere。

简介：ThewildPorphyrayezoensiscollectedfromtheQingdaocoastwasusedtoprepareprotoplastsbyenzymedigestion.Thepurelinewasconstructedbycultivatingtheprotoplasts.The18SrDNAoftheP.yezoensispurelinewasclonedandsequenced.Sequenceanalysiswasexecutedforthissequenceandother22sequencesretrievedfromGenBank.Aphylogenetictreewasconstructedusingtheneighbor-joiningmethod.Theresultsrevealedahighdiversityof18SrDNAsequencesingenusPorphyraandtheconsiderablevariationof18SrDNAsequencesindifferentstrainsofthesamespeciesP.yezoensisandP.tenera.Significantdifferenceof18SrDNAsequencewasobservedbetweenP.yezoensisfromQingdao,China,andthetwostrainsofP.yezoensisfromJapan,butthethreestrainsofP.yezoensisformedastablecladeinthephylogenetictree.TheseresultsindicatethepossibilityofinterspeciesandintraspeciesdiscriminationofPorphyrausingthe18SrDNAsequences.

简介：这研究分析社区结构，水藻的数量变化和重要环境因素的效果并且在调查区域估计依附的绿水藻的全部的生物资源。从Porphyrayezoensis水产业椽子上的依附的绿水藻的调查的学习使用数据和关于到在Subei的2011年4月的从2010年10月的环境因素的数据变浅。椽子上的依附的绿水藻包括了Ulvaprolifera，Capsosiphongroenlandicus，U。linza，U。intestinalis，U。clathrata，和U。compressa。依附的绿水藻的生物资源变化展出了一根转换抛物线：生物资源最高(14898t)在4月，并且第二最高(2034t)在11月；它在2月(仅仅729t)是最低的并且从三月严厉地增加了到4月。种类差异在季节之中显著地不同。在9月和10月，什么时候P。yezoensis水产业椽子开始被建立，，依附的绿水藻从12月有高生物多样性到下一2月，，许多绿水藻种类在椽子上共存了生物资源是低的，并且从三月到4月作为严厉地增加的生物资源，种类差异落下到最小。在期间这次，C。groenlandicus与直到80%的最大的生物资源比例是显然主导的，当时U。prolifera比例指数地增加了到20%～40%。水温度在生物资源和依附的绿水藻的种类继任上有直接调整效果。社区动力学的评价和绿水藻的生物资源提供了需要在南部的黄海追踪大规模绿潮的起源的证据。