简介:Nearisogeniclinescarryinglarge-effectQTL(qtl12.1),whichhasaconsistentinfluenceongrainyieldunderuplanddroughtstressconditionsinawiderangeofenvironments,wereevaluatedunderwaterstressinthefields.Thelinewhichgavehigheryieldunderdroughtwascrossedwithalocaleliteline,PMK3,andforwardedtoF2:3generation.SignificantvariationwasfoundamongtheF2:3linesforagronomictraitsunderwaterstressinthefields.Lowtohighbroadsenseheritability(H)forinvestigatedtraitswasalsofound.Waterstressindicatorssuchasleafrollingandleafdryingwerenegativelycorrelatedwithplantheight,biomassandgrainyieldunderstress.Bulkedsegregantanalysis(BSA)wasperformedwiththemarkersinthevicinityofqtl12.1,andRM27933wasfoundtobesegregatedperfectlywellinindividualcomponentsofdroughtresistantanddroughtsusceptiblebulkswhichwerebulkedbasedonyieldunderwaterstressamongF2:3lines.Hence,thissimpleandbreederfriendlymarker,RM27933,maybeusefulasapotentiallyvaluablecandidatemarkerforthetransferoftheQTLqtl12.1intheregionalbreedingprogram.BioinformaticanalysisoftheDNAsequenceoftheqtl12.1regionwasalsodonetoidentifyandanalyzepositionalcandidategenesassociatedwiththisQTLandtoascertaintheputativemolecularbasisofqtl12.1.
简介:QTLmappingforsevenqualitytraitswasconductedbyusing254recombinantinbredlines(RIL)derivedfromajaponica-japonicaricecrossofXiushui79/CBao.Theseventraitsinvestigatedweregrainlength(GL),grainlengthtowidthratio(LWR),chalkgrainrate(CGR),chalkinessdegree(CD),gelatinizationtemperature(GT),amylosecontent(AC)andgelconsistency(GC)ofheadrice.ThreemappingmethodsemployedwerecompositeintervalmappinginQTLMapper2.0softwarebasedonmixedlinearmodel(MCIM),inclusivecompositeintervalmappinginQTLIciMapping3.0softwarebasedonstepwiseregressionlinearmodel(ICIM)andmultipleintervalmappingwithregressionforwardselectioninWindowsQTLCartographer2.5basedonmultipleregressionanalysis(MIMR).ResultsshowedthatfiveQTLswithadditiveeffect(A-QTLs)weredetectedbyallthethreemethodssimultaneously,twobytwomethodssimultaneously,and23byonlyonemethod.FiveA-QTLsweredetectedbyMCIM,ninebyICIMand28byMIMR.ThecontributionratesofsingleA-QTLrangedfrom0.89%to38.07%.AlltheQTLswithepistaticeffect(E-QTLs)detectedbyMIMRwerenotdetectedbytheothertwomethods.FourteenpairsofE-QTLsweredetectedbybothMCIMandICIM,and142pairsofE-QTLsweredetectedbyonlyonemethod.Twenty-fivepairsofE-QTLsweredetectedbyMCIM,141pairsbyICIMandfourpairsbyMIMR.ThecontributionratesofsinglepairofE-QTLwerefrom2.60%to23.78%.IntheXiu-BaoRILpopulation,epistaticeffectplayedamajorroleinthevariationofGLandCD,andadditiveeffectwasthedominantinthevariationofLWR,whilebothepistaticeffectandadditiveeffecthadequalimportanceinthevariationofCGR,AC,GTandGC.QTLsdetectedbytwoormoremethodssimultaneouslywerehighlyreliable,andcouldbeappliedtoimprovethequalitytraitsinjaponicahybridrice.
简介:本研究利用以0—153为父本和SGK9708为母本构建的196个陆地棉重组自交系(F6:8)为材料对棉籽油分含量和蛋白质含量进行了遗传分析和QTL定位。通过四个环境下的群体材料的棉籽油分含量和蛋白质含量分析表明棉籽油分含量和蛋白质含量均为典型数量性状,其中棉籽油分含量存在超低亲本的超亲分离,而其蛋白质含量呈现超高亲本的超亲分离。相关性分析显示棉籽油分含量和蛋白质含量呈极显著负相关,同步提高两者在棉籽的的含量较为困难。基于包含186个标记,总长827.84cM,标记间平均距离4.45cM,覆盖棉花基因组18.6%的遗传连锁图谱,应用WinQTLcart2.5软件对四个环境下的棉籽油分含量和蛋白质含量进行了QTL定位,共检测到8个油分含量QTLs,解释表型变异5.42%~13.15%,其中稳定的QTL1个。4个蛋白质含量QTLs,解释表型变异4.35%~14.93%。本研究结果可为进行陆地棉种子营养品质性状的分子遗传改良奠定基础。
简介:摘要目的探究血常规检测在临床疾病中的运用价值及意义。方法于2010年2月到2013年3月,抽取我院肺炎患者54例作为观察组,另选取同期来我院检查的健康体检者54例作为对照组。对两组人群的血常规进行检测并对各个指标进行比较。结果观察组病患的血常规检测白细胞计数、中性粒细胞计数,均显著高于对照组;淋巴细胞计数显著低于对照组。差异均有统计学意义(均P<0.05)。观察组病患白细胞轻度升高者、中度升高者、重度升高者以及总升高数者占比均显著高于对照组。差异均有统计学意义(均P<0.05)。结论血常规检测在临床疾病中的检测意义显著,能明确病情,减轻患者痛苦,安全性好,对诊断疾病具有积极意义,值得临床广泛推荐。
简介:基于电务试验车或高速动检车的信号动态检测技术能够准确掌握机车信号显示、轨道电路特性、补偿电容、点式应答器和无线通信等电务设备运用状态,及时发现并预防设备故障。就信号动态检测技术现状、发展方向、原理及典型处理方案进行阐述和分析,探讨信号动态检测技术的实践意义。