简介：Theelectromagneticbalanceheadisusedforbalancingtheelectricspindle.Thispaperanalyzestheinternalstructureoftheelectromagneticbalanceheadandintroducesthestructureofthecounterweightplateandtherangeofthebalanceinthemovingring.Theprincipleofhowtheelectromagneticfieldgeneratedbythestaticcoilactsonthemovingringisalsoinvestigated.ThemodelisbuiltinAnsoftMaxwellandtheexcitationparametersandboundaryconditionsaresetup.Throughtheanalysisofthemagneticfieldgeneratedbythestaticcoilinthepositionofthemovingring,itisverifiedthatthestaticloopcoilcandrivethecounterweightplate.

简介：老鼠卵母细胞在meiotic成熟，和这极化期间经历极化为最大化母亲的部件的保留的不对称的房间部门的必需品为早开发被要求。没有常规中心体，meiotic锭子少些集中了杆并且是塑造桶的。到外皮的meiotic锭子的迁居被外皮的一个本地重组和极化伴随。LGN是涉及房间极性和锭子组织的规定的保存蛋白质。在现在的学习，我们在老鼠卵母细胞成熟期间描绘了LGN的本地化动力学并且在meiotic锭子组织上分析了LGNupregulation和downregulation的效果。在幼芽的泡阶段，LGN细胞质地并且在外皮被散布。在成熟期间，LGN本地化到meiotic锭子仪器，外皮的LGN变得更少在肌动朊帽子区域专注。过多的LGN由伸长导致meiotic锭子组织缺点集中的锭子和提高的杆，而由RNA干扰的LGN的弄空导致meiotic锭子变丑和染色体错误排列。而且，LGN的N终点有全身的LGN的能力调整锭子组织，而LGN的C终点控制外皮的本地化和极化。我们的结果表明LGN外皮地在老鼠卵母细胞被极化并且为meiotic锭子组织是批评的。

简介：Spindlecellcarcinomaofthebreastisararetumor.Thistumorcanproliferaterapidlyandcausecysticchangesbecauseofinternaltissuenecrosis.Weevaluateda54-year-oldwomanwithrightbreastlump.Mammographyshowedacategoryfourmasswithadiameterof2.5cm.Ultrasonography(US)revealedacomplexcysticlesion,andfine-needleaspiration(FNA)cytologydemonstratedbloodyfluidandmalignantcells.Partialbreastresectionandsentinellymphnodebiopsywereperformed.Immunohistologyrevealedspindlecellswithpositiveresultsforcytokeratin(AE1/AE3)andvimentin,partiallypositiveresultsfors-100,andnegativeresultsfordesminandα-actin.ThepathologicalstagewasIIA,andbiochemicalcharacterizationshowedthatthetumorwastriplenegative.SixcoursesofFEC-100chemotherapy(5-fluorouracil500mg/m2,epirubicin100mg/m2,andcyclophosphamide500mg/m2)wereadministered.Radiotherapywasperformed.Thiscaseisdiscussedwithreferencetotheliterature.

简介：Thecharacteristicsoftheparticulatemousecentromereenrichedfractionfromisolatednucleiobtainedinourlaboratorywereinvestigatedbyindirectimmunofluorescence,testoftheactivityofmicrotubuleorganizingcenter(MTOC),SDS-PAGE,andfluorescenceinsituhybridization.MostoftheparticlesofthefractionarecomplexesofDNAandkinetochoreproteinsandshowMTOCactivity.TheDNAisolatedfromthefractioncanhybridizewithDNAintheregionsoftheprimaryconstrictionsofallchromosomesofascionsoftheprimaryconstrictionsofallchromosomesofascitescells.Thekinetochoreproteinsisolatedfromthefractionaremainlythosewithmolecularweightof55KDand59KD.Resultssuggestedthatthefractionobtainedisacentromereenrichednuclearfractionasindicatedinourpreviousreport.

简介：AbstractObjective:The meiotic spindle controls chromosome movement and mediates various functions essential for fertilization and early postfertilization events. This study aimed to examine whether vitrification causes meiotic damage in vitro-matured metaphase II (MII) human oocytes, and whether the meiotic spindle morphology influences the subsequent developmental outcomes.Methods:The spindle characteristics of MII human oocytes in vitro matured were studied before and after vitrification using PolScope imaging and immunofluorescence staining. The developmental competence of oocytes was also examined.Results:A total of 419 human MII oocytes were obtained from 593 intracytoplasmic sperm injection cycles at our hospital. Of these oocytes, 54 were used for immunofluorescence staining, whereas the other oocytes were examined by PolScope imaging and classified into three groups according to the meiotic spindle morphology: (A) normal morphology, (B) weak refraction and short meiotic spindle, and (C) no detectable meiotic spindle. The three groups demonstrated statistically significant differences in terms of survival after vitrification. However, differences were not found in terms of oocyte chromosome structure and meiotic spindle morphology on immunofluorescence staining performed before and after vitrification. Oocyte survival, fertilization, and early embryonic development rates were significantly higher in Group A than in Groups B and C with or without vitrification. While vitrification had no effect on these metrics in Group A, Groups B and C demonstrated significantly lower fertilization and cleavage rates after vitrification/warming.Conclusions:Screening for normal meiotic spindle morphology and chromosome configuration before vitrification may increase the yield of healthy viable oocytes for various assisted reproductive technologies.

简介：TheprecisionforgingprocessissimulatedbycommercialsoftwareDeform3Dusingarigidvisco-plasticmodeltopredictthestatusofmetalflowandthedistributionofequivalentplasticstrain,providingguidanceformakingdecisionontheoptimalchoiceofprocessparametersandmouldstructure.TrialforgingwasusedtoverifytheeffectivenessofFEMsimulationresults.

简介：Thewell-distributionspindleLiFePO4(LFP)nanoparticlesascathodeoflithiumsecondarybatteriesweresynthesizedbyasolvothermalreactionrouteatlowtemperature(180°C)inwhichtheascorbicacidwasusedasreducingagent.InordertoguaranteethatthepHvaluesofthermalsystemswerenotaffectedtoomuchandthereducibilityofthesystemwasenhancedatthesametime,glucosewaschosenasanauxiliaryreductantinthisreaction.TheobtainedpowderswerecharacterizedbyX-raydiffraction(XRD),scanningelectronmicroscopy(SEM),andlaserparticleanalyzer.Theresultsshowthatthecarbon-coateduniformspindleolivineLiFePO4/C-glucoseparticles(glucoseasauxiliaryreductant,LFP/C-G)arepreparedwiththesize500–600nmandwithoutanyimpurityphases.Theirelectrochemicalpropertieswereevaluatedbyelectrochemicalimpedancespectroscopy,cyclicvoltammetry,andgalvanostaticcharge/dischargetests.LFP/C-Ghasahigherconductivityandbetterreversiblecapabilitythancarbon-coatedLFP(LFP/C).ThehighestdischargecapacityofLFP/C-Gis161.3mAh·g-1at0.1Cand108.6mAh·g-1at5.0C,respectively.TheresultsimplythattheneatcrystalnanostructureofLFP/C-Ghasexcellentcapacityretentionandcyclingstability.Theaddingofglucoseisthekeyfactorforthewelldistributionandneatcrystalstructureofnanoparticles,thustheelectrochemicalperformancesofmaterialsareimproved.

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简介：Theauthorsinvestigateadisturbance-compensatingandenergy-savingcontrolmethodforairturbinespindlesequippedwitharotationcontrolsystemdesignedforuseinultra-precisionmilling.Thecontrollabilityandenergy-conservingcharacteristicsoftheproposedmethodusingtheproposedpneumaticregulatingdevice,calledahigh-precisionquick-responsepneumaticpressureregulator,weredemonstratedexperimentallypreviously.Herein,theproposedrotation-controlledairturbinespindleisfirstsummarised.Subsequently,theproposedrotation-controlledairturbinespindleissettoamillingmachineandappliedtothemillingofanalloytoolsteel,togaindataandvalidatetheeffectivenessofthein-processtoolwearestimationmethodthathavebeenrecentlydevelopedandproposedbytheauthors.Particularly,toevaluatethepossibilityofapplyingtheproposedestimationmethodtothree-dimensionalmilling,millingexperimentswheretheairturbinespindleissetata45°inclinewereconductedandtheresultsindicateahighpossibilityoftheproposedmethod.

简介：Theauthorsinvestigateadisturbance-compensatingandenergy-savingcontrolmethodforairturbinespindlesequippedwitharotationcontrolsystemdesignedforuseinultra-precisionmilling.Thecontrollabilityandenergy-conservingcharacteristicsoftheproposedmethodusingtheproposedpneumaticregulatingdevice,calledahigh-precisionquick-responsepneumaticpressureregulator,weredemonstratedexperimentallypreviously.Herein,theproposedrotation-controlledairturbinespindleisfirstsummarised.Subsequently,theproposedrotation-controlledairturbinespindleissettoamillingmachineandappliedtothemillingofanalloytoolsteel,togaindataandvalidatetheeffectivenessofthein-processtoolwearestimationmethodthathavebeenrecentlydevelopedandproposedbytheauthors.Particularly,toevaluatethepossibilityofapplyingtheproposedestimationmethodtothree-dimensionalmilling,millingexperimentswheretheairturbinespindleissetata45°inclinewereconductedandtheresultsindicateahighpossibilityoftheproposedmethod.

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