简介：Nutrient-poor,sandysoilsformtheprevailingsubstrateatpost-miningsitesoftheLusatianregion(Brandenburg,Germany)andpresentachallengeforvegetationdevelopment.Westudiedtheorganicacidquantityandcompositionofthreecommonlyoccurringpioneerplantspecies,thelegumesLotuscorniculatusL.andTrifoliumarvenseL.andthegrassCalamagrostisepigeios(L.)Roth,todetermineifplantgrowthandexudationdifferedwith(non-sterilizedsoil)andwithout(sterilizedsoil)anindigenoussoilmicrobialcommunity.Weinvestigatedwhetherorganicacidswerefoundintherhizosphereandsurroundingsoilandwhetherthisinfluencednutrientmobilization.Thisstudyconsistsoflinkedfieldinvestigationsandagreenhouseexperiment.Plantsweregrowninthegreenhouseineithersterilizedornon-sterilizedsandysoilfromareclamationsiteintheLusatianmininglandscape(WelzowSu¨d,EastGermany).Aftersevenmonths,theplantbiomass,rootmorphology,organicacids,andwater-solublenutrientsandrootcolonizationwitharbuscularmycorrhizalfungi(AMF)anddarkseptateendophytes(DSE)wereanalyzed.RootsofallthreeplantsinthefieldandgreenhouseexperimentswerehighlycolonizedwithAMF.CalamagrostisepigeiosandT.arvensehadasignificantlyhighercolonizationfrequencywithDSEthanL.corniculatus.Thequantityandcompositionoforganicacidsstronglydifferedamongplantspecies,withthehighestnumberoforganicacidsfoundforL.corniculatusandlowestforC.epigeios.Thequantityoforganicacidswasgreatlyreducedinallplantsundersterilizedsoilconditions.However,thecompositionoforganicacidsandplantgrowthinsterilizedsoilwerereducedforbothlegumes,butnotforC.epigeios,whichhadahigherbiomassundersterilizedconditions.Changesinnutrientconcentrationsinthefieldrhizospheresoilrelativetothoseinthecontrolweremeasurableaftersevenmonths.Whilethespectrumoforganicacidsandthegrowthoflegumesseemedtobedependentonahighly

简介：由把OsRacDcDNA用作探查屏蔽光周期的genomic图书馆敏感妖怪男性无菌的米饭行Nongken58S，包含编码OsRacD的区域的2kb倡导者and396bp的积极克隆被获得。与reversePCR从正常米饭变化Nongken58(Nongken58N)克隆的OsRacD的倡导者相比，相同是99.8%，并且不同核苷酸在在倡导者的预言的反应元素外面，建议在在长天的条件下面的米饭变化Nongken58S和Nongken58N之间的富饶是没在OsRacD在上游的规定序列的结构归因于差别，但是到基因的发展规定微分表示。

简介：理解photoperiod/thermo-sensitive的男绝育机制遗传因子的男性无菌[P(T)公克]在米饭的线，光周期或温度的遗传上的研究进步在米饭的敏感遗传因子的男绝育被考察。一个新想法被建议解释P(T)公克米饭的绝育机制。富饶转变从对无菌肥沃是有光周期或温度的主要效果的无菌的基因的合作规定的结果敏感基因，然而并非在P(T)公克米饭的所谓的pgms基因。次要效果的基因，在绝育上展出积聚的效果，是为绝育转变的批评温度的重要因素。无菌的线保持越多次要效果的基因，越多降低绝育转变的批评温度。如果所有次要效果的基因是同型结合的，绝育转变的批评温度将是不变的。为引起P(T)公克米饭的策略也被建议。为绝育转变的批评光周期和温度的选择索引应该被放根据品种的类型和生态的区域。强加选择压力是为为绝育与更低的批评温度引起P(T)公克米饭的一种关键技术，并且改进整个特点和联合能力的全面表演为引起P(T)公克米饭线是重要的。

简介：Thisstudywasconductedtodevelopandcharacterizeanovelcytoplasmicmalesterile(CMS)sourcewhichwasidentifiedfromDongxiangwildrice(Oryzarufipogon)bycrossingDongxiangwildriceasfemalewithZhongzao35,anindicainbredvariety,asmaleandcontinuousbackcrossingwithZhongzao35.ObservationunderopticalmicroscopemanifestedthatthisnovelCMSbelongedtotypicalabortiontypewithlesspollencomparedwithwildabortivetypecytoplasm(CMS-WA).SequentialplantingshowedthatthisnovelCMShascompleteandstablemalesterility.Testcrossexperimentshowedthatallthe24testedmaterialsincludingmaintainerandrestorerlinesofCMS-WAandHongliantypecytoplasm(CMS-HL)andotherindicainbredvarietiesarethemaintainerswithcompletemaintainingability,suggestingthatthisnovelCMShasfertilityrestorationtotallydifferentfromCMS-WAandCMS-HLandbelongstoanoveltypeofCMS.Sofar,weonlydiscoveredauniquefertilityrestorationsourceforthisnovelCMS.InheritanceanalysisshowedthatthefertilityrestorationofthisCMSwasgovernedbythreepairsofindependentdominantgenes.ProspectforapplicationofthisnovelCMSsysteminhybridricebreedingwasalsodiscussed.

简介：植物温度(Tp)和它与米饭殖民地和灌溉水的小气候的关系用thermo敏感的遗传因子的男性被学习无菌(TGMS)米饭线，Pei'ai64S。在温度的每日的变化的重要差别在150厘米(TA)的高度在Tpand空气温度之间被检测。从8:00到20:00，Tp比TA，而是他们在到下7:00的21:00期间是类似的的低。最大的Tp比最大的TA一个小时早发生了，尽管他们两个都在6:00到达了最小。Tp波动不到TA。在一样的高度，在6:0013:00期间，Tp比空气温度(Ta)高，并且Tp比Ta一个小时早到达了最大值。在在阳光充足的白天的剩余的时间期间，到近或甚至有点的Tpwas比Ta降低。在阴暗白天，比在整个天内的Ta高的Tpwas，和两个同时最大化。另外，Tpwas在夜里在白天由太阳的放射，云量和风速度，并且由灌溉水调整了。现在的学习显示了那29.6甠?潴?的TA?慥獲τ

简介：包括16不育系的米饭isonuclear-alloplasmic线和他们的维护者线的四个集合被使用91份SSR教材学习原子染色体和他们的相对关系的基因差异分析。169等位基因的一个总数在16根线被检测，与53.85%的多态的loci和每1.8的地点的等位基因的一个平均数字的频率，并且平均基因差异是0.228。无菌的线分享了的isonuclear-alloplasmicmale的四个集合146相同等位基因，相应于86.39%全部的等位基因；同时，在测试材料之中有23不同等位基因，是13.61%全部的等位基因。平均，78.70%相同等位基因和全部的等位基因的21.30%不同等位基因在isonuclear-alloplasmic不育系和他们的维护者线之间被检测。Therewere53.85%相同等位基因和在homozygousallonucleus不育系之中的全部的等位基因的46.15%不同等位基因。指纹为某不育系和维护者线被建立。测试的所有材料基于簇分析在0.2遗传距离被划分成三个组。HuanongA和HuayuA(包括的Huanong乐队HuayuB)的八根线在第一个组，KezhenA的四根线(包括KezhenB)在在第三个组的Zhenshan97A(包括的Zhenshan97B)的第二个组，和四根线。为theisonuclear-alloplasmic不育系，在Y(Yegong)类型和WA之间的类似系数(野未成功)打字或在CW(Raoping野米饭)和WA之间打到达87-98%。

简介：TheDNAfragmentsabout1600bpwereamplifiedusingrandomamplifiedpolymorphismDNA(RAPD)primerOPA12withthetemplatesofmitochondrialDNAofZhenshan97AandZhenshan97B,andweresequenced.ThenucleotidesequencesandlengthsofthefragmentsfromZhenshan97AandZhenshan97Bshowednodifference.Thepreciselengthofthefragmentwas1588bp.Sequencecharacterizedamplificationregion(SCAR)primerswerethendevelopedtodiscriminatethecytoplasmicmalesterile(CMS)linesandtheirmaintainerlines.Aspecific1588bpfragmentcouldbeamplifiedwithSCARprimers,CHI19F2/CHI19R2andCHI20F3/CHI23R3,inthemitochondrialDNAofZhenshan97A,butnotZhenshan97B.Furthermore,thespecificfragmentcouldbealsoamplifiedfromthetotalDNAfromgreenleaftissuesofZhenshan97AwithSCARprimers,butnotZhenshan97B.Withthecorrespondingprimers,thespecificfragmentcouldalsobeamplifiedfromthetotalDNAofgreenleavesofothertwoCMSlineswithwildabortivetypecytoplasm(CMS-WA),namelyZhenpinAandTianfengA,butnotintheirmaintainerlines.Moreover,usingtotalDNAastemplate,eachofthefourpairsofSCARprimerscouldalsobeusedtoamplifythe1588bpfragmentinCMS-ID(Indonesiapaddytype)lineⅡ-32A,butnotinII-32B,andthespecificfragmentwasamplifiedfromtheDNAofbothF1andF2seedlingsofShanyou63.Theresultsofdetectingthegeneticpurityofaman-mademixtureoftheseedsofZhenshan97AusingCHI20F3/CHI23R3werecompletelyconsistentwiththephenotypes.Takentogether,theseresultsindicatedthatthespecific1588bp-fragmentamplifiedbyCHI20F3/CHI23R3wastheuniqueamplificationproductsofCMSmitochondrialDNA,andcouldbeusedtodistinguishCMS-WAandCMS-IDlinesfromtheircorrespondingmaintainerlinesattheseedlingstage.

简介：为了在不同发育阶段调查织物transglutaminase活动，叶子和米饭的年轻圆锥花序的变化，从Honglian-typecytoplasmic不育系，YuetaiA和它的维护者线被切除，YuetaiB分别地。为在米饭的织物transglutaminase活动察觉的一个ELISAmeasurement协议很好被建立。结果显示织物transglutaminase活动被钙阳离子，和织物transglutaminase活动在衰老的叶子断然调整在年轻叶子比那显著地高。没有不同差别在YuetaiA和YuetaiB之间被注意。而且，从四个起核心作用到双性人织物transglutaminase活动逐渐地增加了的阶段年轻圆锥花序发展并且直到在双性人的最大值的withtheprogression起核心作用在YuetaiA.However的舞台，没有类似的变化在YuetaiB被观察。这显示tissuetransglutaminase涉及房间在败育花粉的规划死亡。

简介：Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.

简介：YuetaiA的种子样品，一种Honglian(HL)类型细胞质的男性无菌(厘米)在混合大米的线被调查估计种子纯净并且由在南京，江苏省和Lingshui模仿生物沾染物到YuetaiA分析离开类型植物的原因，海南省在20042006期间。YuetaiA的种子杂质主要源于在生物污染以后然而并非从它的绝育unstability恢复富饶基因(Rf)的基因飘移。十根维护者线，五根restorer线和三thermo敏感的遗传因子的男性，无菌的线在学习使用了的所有能传花粉给生产F1的YuetaiA和YuetaiB种，直接或间接地导致漂流进YuetaiA并且产生iso细胞质恢复产生的Rf基因。而且，高outcrossing率和有许多变化的YuetaiA的类似的标题日期容易在米饭生产力量使用了在生物污染的结果。在移开所有植物，Rf基因在YuetaiA混合了并且阻止Rf基因漂流进YuetaiA以后，YuetaiA和YuetaiB的种子纯净被提起了到100%。

简介：RiceisanimportantfoodcropinChina,andthedevelopmentofhybridriceisacrucialwaytoincreasegrainyield.Thecreationofdual-purposenuclear-sterilelinesfortwo-linehybridbreedinghasbecomevitalforcommercialricebreeding.WeconstructedthepC1300-2x35S::Cas9-sgRNAPTGMS2-1expressionvectorforeditingthemalefertilitygenePTGMS2-1intwowidelycompatiblericevarieties,93-11andHuazhan,byusingtheCRISPR/Cas9system.Weobtainedthemarker-freephotoperiod-/thermo-sensitivegenicmale-sterile(P/TGMS)linesinT1generation.Accordingtotheexperimentsinphytotronwithfourtemperatureandphotoperiodtreatments,wefoundthetemperatureisthemainfactorforrestoringthepollenfertilityofptgms2-1mutantsin93-11andHuazhan,andthephotoperiodalsohassomeeffectsonpollenfertilityintwodifferentricebackgrounds.Theapplicationofcultivatingnewmale-sterilelinesbygenomeeditingsystemwillsignificantlyacceleratethericebreedingprocess.