简介:Eucalyptusisveryrecalcitranttoinvitroculture.Inthisresearch,anefficientshootorganogenesissystemwasdevelopedusing60-day-oldplantsofEucalyptusglobulusgrowninvitroandnon-aeratedliquidmediumtoimproveshootproliferation.Cultureswereinitiatedwithhypocotylsandleafsegmentsfromplantletscultivatedonsemisolid1/2MSmodifiedmediumsupplementedwith4.44lM6-Benzyladenine(BA)and16.1lM1-Naphthaleneaceticacid(NAA).Calliweretransferredtoshootinductionmedium,witheither0.5or2.7lMNAA.Shootmultiplicationwascarriedouton4.44lMBA+0.5lMNAAmedium,andsemisolidandnon-aeratedliquidsystemswerecomparedforimprovingshootproliferation.RootingofadventitiousshootswasevaluatedonmediumcontainingNAAorIndole-3-butyricacid-IBA(5and16lM).Callogenesiswasobtainedfrombothtypesofexplants,althoughshootformationwasonlyobtainedfromleaf-derivedcalli.Shootproliferationon4.44lMBA+0.5lMNAAresultedinthemostshoots/callus.Non-aeratedliquidmediumwasmoreefficientinpromotingshootmultiplication(53.5shoots/callus)thanwassemisolidmedium(28.5shoots/callus).Levelsofphenoliccompoundsweresignificantlyreducedintheshootscultivatedinliquidmedium.Efficientrooting(76%)wasobtainedusing16lMIBA.
简介:Airentrainmentisknowntobeoneofefficientandinexpensivemethodstopreventcavitationdamagesinhydropowerprojects.Theshapeofsuddenexpansion-fallisusedasacommondeviceformitigatingcavitationerosions.Thecomplexflowpatternswithcavitationarenumericallysimulatedbyusingtherealizablek-εturbulencemodelandtheair-watermixturemodel.Thecalculatedresultsarecomparedwellwiththeexperimentalresultsaswellasthoseobtainedwiththek-εturbulencemodelwiththeVolumeOfFluid(VOF)Model.Thecalculatedresultsagreewellwiththeexperimentaldatafortheaerationcavityandwallpressure.Moreover,theairconcentrationnearsidewallissimulatedbyamixturemodel.ItisfoundthatthemixtureturbulencemodelissuperiortotheVOFturbulencemodel.