简介：Thesunisinarage.SincetheendofOctober,strongsolar（ofthesun)stormshavebeen（36）theearth,showingusthe37ofspaceweather.

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简介：Thispaperpresentsastudyoftheeffectofcultur-albackgroundonTOEFLscores.Itproceedsfromtherelationbetweencultureandlanguage,thenillus-trateswithactualquestionsfromvarioussectionsofTOEFLtestshowAmericanculturalbackgroundexertsaremarkableinfluenceonTOEFLscores,andcon-cludeswithrevelationswithregardtoEnglishteachinginthiscountry.

简介：Despitethefactthatironisoneofthemostabundantelementsoftheearth'scrust,irondeficienciesareseriousproblemsbothinhumannutrition[1]andinagriculture[2].Sixtoeightpercentoftheworld'spopulationispotentiallyaffectedbyirondeficiencyinducedanemia,aleadingcauseofmaternaldeathinAfricanandAsiancountrieswherepeoplerelymostlyonplantsfortheirdailyintakeofiron.Ironcanalsobealimitingfactorinthegrowthofeconomicallyimportantcropplantsbecauseofinadequatesoilchemistry,andsuchdeficienciescannoteasilybecorrectedbyamendingthesoil.Improvingtheplant'sabilitytoabsorbironinadverseconditionsandtoincreasetheiroverallcontentcouldoffersolutionstothesedramaticproblems.Thereforeunderstandingthemolecularmechanismsregulatingironuptakeandhomeostasisinplantshaspotentiallyimportantpracticalapplicationsbothinagricultureandhumanhealth[3].

简介：Hydrolyzedleather,obtainedfromindustrialleatherwaste,isanorganicnitrogenousfertilizerwithahighchromium(Cr)content.SoybeancropscanbeadverselyaffectedbythepresenceofresidualCrinsoilafterapplicationofhydrolyzedleatherfertilizer.TheaimofthisworkwastoassesstheeffectofhydrolyzedleatheronCrabsorption,rootdevelopment,andsymbioticnodulationofsoybeanplantsgrownonthreedifferentsoilsincludingtwoLixisolsandaGleysol.Thesesoilswerepreviouslyfertilizedwithhydrolyzedleatheratthreedifferentrates(0.0,0.1,and0.8kgm-2).Soybeanshootheight,rootdevelopment,nodulation,andmineralnutrientconcentrationsinplanttissuesweredeterminedafter45dofcropgrowth.TheapplicationofhydrolyzedleathersignificantlyincreasedCrcontentinsoilandCruptakebyplants.Surprisingly,Crwasstoredmainlyintheaerialpartoftheplants.ThepresenceofCrhadanadverseimpactonshootdrymatter(41%reduction)andnumberofnodules(49%reduction)perplantgrownonthesoilwiththelowestcationexchangecapacity.However,noothersignificanteffectwasobservedinnoduleweight,diameter,oranyothermeasuredparameters.

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简介：Background:Novelplayertrackingtechnologiescanchangetheunderstandingofperformancedeterminantsinteamsportsbyallowingtoaccuratelymeasuringtheactivitydemands.Theaimofthisstudywastoidentifyhowthedefensivepressureaffectsthetime-motionvariablesandthetechnicalactionsinbasketball.Methods:Twentyinternationalmaleplayers(age:16.05±2.09years,weight:73.13±8.10kg,height:183.10±5.88cm)playedtwo10minbasketballquarters,wheretheyusedaman-to-man1/4-courtdefenseuntilthe4thmin(F1/4),changedtoman-to-manfullcourt(FULL)for3minand,fromthe7thtothe10thminreturnedto1/4-courtdefense(S1/4).AcomputerizednotationalanalysiswasperformedusingSimiScoutandpositionaldatawerecapturedwiththeUbisenseRealTimeLocationSystem(meansamplingrate3.74±0.45Hzpertransmitter/player).Results:Thetime-motionvariablespresentedsimilarresultsbetweendefensiveconditions,showingatotaldistancecoveredaround90m/min.However,resultssuggestedpossibleverticaljumpimpairmentsinS1/4periods.Therewasmoredistancecoveredwhilejoggingintheoffensivecourt(38.15±12.17m/minoffensivecourtvs.32.94±10.84m/mindefensivecourt,p<0.05)andmoredistancecoveredwhilerunninginthedefensivecourt(16.41±10.27m/minoffensivecourtvs.19.56±10.29m/mindefensivecourt,p<0.05).Conclusion:Theseresultssuggesthowtoimprovetaskrepresentativenessduringspecificconditioningorgame-basedtrainingsituationsandalsotohelpcoaches’strategicdecisionsduringthegames.

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简介：昆虫手足在变形期间从想象的圆盘或幼虫的腿发展。涉及发展从的分子的机制对成年的腿幼虫糟糕被理解。此处，我们克隆从Bombyxmori(Bmrn)圆胖的锌手指基因的完整的长度，它包含了1419bp开的读框架，并且编码了473氨基酸蛋白质。反向的抄写聚合酶链反应和西方的污点分析证明Bmrn比在测试的另外的纸巾在外皮在高水平被表示，并且它在变形期间显示出很高的表示水平。生产的Bmrn击倒在跗骨和pretarsus背叛包括tarsomeres的熔化和减小，并且pretarsus的发展拘捕。而它在果蝇的相应基因被显示了影响三个跗骨的片断(t2-t4)，我们的数据证明Bmrn涉及跗骨和pretarsus的形成，建议改变腿包含了在在进化期间的基因规定的patterning的变化。

简介：γ-AminobutyricacidandGABAergicreceptorswerepreviouslyreportedtobedistributedinreproductivesystemsbesidesCNSandpredictedtoparticipateinthemodulationoftesticularfunction.γ-Aminobutyricacidtransporterwasimplicatedtobeinvolvedinthisprocess.However,thepotentialroleofγ-aminobutyrictransporterintestishasnotbeenexplored.Inthisstudy,weinvestigatedtheexistenceofmouseγ-aminobutyricacidtransportersubtypeI(mGAT1)intestis.Wild-typeandtransgenicmice,whichoverexpressingmGAT1inavarietyoftissues,especiallyintestis,wereprimarilystudiedtoapproachtheprofileofmGAT1intestis.MicewithoverexpressedmGAT1developnormallybutwithreducedmassandsizeoftestisascomparedwithwild-type.Testicularmorphologyoftransgenicmiceexhibitedovertabnormalitiesincludingfocaldamageofthespermatogenicepitheliumaccompaniedbycapillariesproliferationandincreaseddiameterofseminiferoustubuleslumen.Reducednumberofspermatidswasalsofoundinsomeseminiferoustubules.OurresultsclearlydemonstratethepresenceofGAT1inmousetestisandimplythatGAT1ispossiblyinvolvedintesticularfunction.

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简介：AbstractBackground:Ubiquitin-conjugating enzyme E2C (UBE2C) has been shown to be associated with the occurrence of various cancers and involved in many tumorigenic processes. This study aimed to investigate the specific molecular mechanism through which UBE2C affects breast cancer (BC) proliferation.Methods:BC-related datasets were screened according to filter criteria in the Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database. Then differentially expressed genes (DEGs) were identified using Venn diagram analysis. By using DEGs, we conducted the following analyses including Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), protein-protein interaction (PPI), and survival analysis, and then validated the function of the hub gene UBE2C using quantitative reverse transcription-polymerase chain reaction (RT-qPCR), cell counting kit-8 (CCK-8) assay, transwell assay, and Western blot assay.Results:In total, 151 DEGs were identified from the GEO and TCGA databases. The results of GO analysis demonstrated that the DEGs were significantly enriched with mitotic nuclear division, lipid droplet, and organic acid-binding. KEGG analysis showed that the peroxisome proliferators-activated receptor (PPAR) signaling pathway, regulation of lipolysis in adipocytes, and proximal tubule bicarbonate reclamation were significantly enriched in the signal transduction pathway category. The top three hub genes that resulted from the PPI network were FOXM1, UBE2C, and CDKN3. The results of survival analysis showed a close relationship between UBE2C and BC. The results of CCK-8 and transwell assays suggested that the proliferation and invasion of UBE2C knockdown cells were significantly inhibited (P < 0.050). The results of Western blot assay showed that the level of phosphorylated phosphatase and tensin homology deleted on chromosome 10 (p-PTEN) was obviously increased (P < 0.050), whereas the levels of phosphorylated protein kinase B (p-AKT), phosphorylated mammalian target of rapamycin (p-mTOR), and hypoxia-inducible factor-1 alpha (HIF-1α) were dramatically decreased (P < 0.050) in the UBE2C knockdown cell.Conclusion:UBE2C can promote BC proliferation by activating the AKT/mTOR signaling pathway.

简介：AbstractObjective:The objective of this study was to investigate the expression levels of microRNA-141-5p(miRNA-141-5p), MAPK1 and neutrophil elastase in patients with and without preeclampsia (PE), and the relationship between miRNA-141-5p and MAPK1 with respect to the secretion of elastase by neutrophils in patients with PE.Methods:Thirty patients with PE and 30 healthy pregnant (HP) women were recruited from The Second Hospital of Shanxi Medical University, Taiyuan, China, between February 2017 and July 2018. Neutrophils were isolated from 8 mL peripheral blood samples and cultured. We recorded neutrophil count and morphology during culture. Apoptosis was detected by flow cytometry in different groups at 0, 24, and 48 h. The expression levels of elastase were detected in neutrophils by enzyme-linked immunosorbent assay, whereas the expression levels of miRNA-141-5p in peripheral blood neutrophils were detected by real-time polymerase chain reaction. We used TargetScanHuman Release 7.2 to analyze the target genes of miRNA-141-5p. The expression of MAPK1 in peripheral blood neutrophils was detected by western blotting. Data were analyzed by SPSS version 21.0 software, and comparisons between groups were carried out with the Student t test.Results:There was no significant difference between the PE and HP groups (P > 0.050) with regard to age or body mass index. The weight of newborns in the PE group (2846.00 ± 600.00 g) was significantly lower than that in the HP group (3055.00 ± 230.68 g). The number of neutrophilic granulocytes(NGs) in blood samples from the PE group was significantly higher than that in the HP group (P = 0.003). There was no significant difference between the groups with regard to morphology. Apoptosis in the PE group was delayed when compared with the HP group at different time points. The P value of apoptosis in the PE and HP groups were respectively 0.790, < 0.001 and 0.030 at 0 h, 24 h and 48 h. The expression levels of miRNA-141-5p in the PE group were significantly lower than those in the HP group (P < 0.050). The expression levels of MAPK1 in neutrophils from the PE group were significantly higher than those in the HP group (P < 0.050) by western blot. The expression levels of elastase in neutrophils from the PE group were significantly higher than those in the HP group (P < 0.050). Furthermore, the number of NGs in peripheral blood from the PE group was higher than that of the HP group; however, the levels of apoptosis were lower. The expression levels of miRNA-141-5p in NGs decreased, the expression of MAPK1 increased, and the secretion of neutrophil elastase in the NG medium increased in the PE group than those in the HP group.Conclusion:Collectively, our analysis suggested that miRNA-141-5p may be involved in the pathogenesis of PE by regulating the MAPK1 signaling pathway to activate neutrophils and increase the secretion of elastase.

简介：Purpose:Thepurposeofthisstudywastocomparethecoordinationbetweenthetrunkandthepelvisduringasustainedasymmetricrepetitiveliftingtaskbetweenagroupwithahistoryoflowbackpain(LBP;HBP)andagroupwithnohistoryofLBP(NBP).Methods:Volunteerslifteda11-kgboxfromankleheightinfronttoashelf45°off-centeratwaistheight,andloweredittothestartpositionat12cycles/minfor10min.Liftingsidewasalternatedduringthetrial.Continuousrelativephasewasusedtocalculatecoordinationbetweenthepelvisandtrunkrotationatthebeginning(Min1),middle(Min5),andendofthebout(Min9).Results:Whiletherewerenomaineffectsforgroup,asignificantinteractionbetweentimeandgroupindicatedthat,inthefrontalplane,theNBPgroupcoordinationwasmoreanti-phasetowardtheendofthebout,withnosuchdifferencesfortheHBPgroup.Analysisofsagittal-axial(bendandtwist)coordinationrevealedtheHBPgroupcoordinationwasmorein-phaseattheendoftheboutovertheentirecycleandfortheliftingphasealone,withnosuchdifferencesfortheNBPgroup.Conclusion:DifferencesbetweengroupsdemonstrateresidualconsequencesofLBPinanoccupationalscenario,eventhoughtheHBPgroupwaspain-freefor>6monthspriortodatacollection.Morein-phasecoordinationintheHBPgroupmayrepresentacoordinationpatternanalogousto'guardedgait'whichhasbeenobservedinotherstudies,andmaylendinsightastowhytheseindividualsareatincreasedriskforre-injury.

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简介：昆虫的embryonic以后开发高度依赖于ecdysteroid荷尔蒙20-hydroxyecdysone。万圣节前夕基因更鬼(spok，cyp307a2)被记录了在果蝇melanogaster和Bombyxmori涉及ecdysteroidogenesis。我们这里描述克隆和万圣节前夕基因的描述更鬼(Lsspok，Lscyp307a2)在小棕色的planthopperLaodelphaxstriatellus，hemipteran昆虫种。LsSPOK有三个保存昆虫的P450主题，也就是说Helix-K，PERF主题和heme有约束力的领域。Lsspok的时间、空间的表示模式被量的聚合酶链反应评估。通过fouth中间形态和早第五中间形态的阶段，Lsspok显示出二表情在秒天和第五天的第四中间形态的美女的山峰，和二马槽在第一天第四和第五中间形态。在白天5第四中间形态的美女上，Lsspok清楚地在prothoracic腺被定位的胸有高抄本水平。在美女阶段的Lsspok的双strandedRNA的饮食的介绍成功地将目标基因击倒，蜕皮激素的减少的表达式水平受体(LsEcR)基因，引起的nymph的致命性和推迟的开发。在Lsspok-dsRNA-exposed美女的20-hydroxyecdysone的摄取没增加Lsspok表示水平，但是几乎完全救LsEcRmRNA水平并且在幸存和发展上减轻否定效果。因此，我们的数据建议ecdysteroidogenic小径在昆虫被保存，LsSPOK为在在L的ecdysteroidogenesis的特定的步负责。striatellus。

简介：BACKGROUND:Thestellateganglionblock(SGB)playsaprotectiveroleinfocalcerebralischemia/reperfusioninjury.ThehumanSGBcanbesimulatedbytransectionofthecervicalsympathetictrunk(TCST)inrats.OBJECTIVE:ToobservetheeffectsofTCSToninduciblenitricoxidesynthase(iNOS)levelsandcerebralinfarctvolumeinthehippocampusofratswithcerebralischemia/reperfusioninjury,andtoanalyzethemechanismofaction.DESIGN,TIMEANDSETTING:Acompletelyrandomized,controlled,neuropathologicalexperimentwasperformedattheInstituteofNeurologicalDisease,TaiheHospital,YunyangMedicalCollegebetweenMarchandSeptember2006.MATERIALS:Atotalof93Wistarrats,aged17-18weeks,ofeithergender,wereusedforthisstudy.2,3,5-triphenyltetrazoliumchloridewaspurchasedfromChangshaHongyuanBiologicalReagentCompany,China.RabbitiNOSantibodyandgoatanti-rabbitIgGantibodyweretheproductsofWuhanBosterBiologicalReagentCo.,Ltd.,China.METHODS:Tenratswererandomlyselectedforthesham-operatedgroup.Cerebralischemia/reperfusioninjurywasinducedbymiddlecerebralarteryocclusion(MCAO)usingthesuturemethodintheremainingrats.Fortysuccessfulratmodelswererandomlyandequallydividedintothefollowingtwogroups:(1)TCSTgroup:subsequenttoTCST,MCAOwasperformedfor2hours,followedby24hoursreperfusion;(2)modelgroup:ratsunderwentexperimentalproceduressimilartotheTCSTgroup,withtheexceptionofTCST.Ratsinthesham-operatedgroupweresubjectedtoexperimentalproceduressimilartothemodelgroup;however,thethreadwasonlyintroducedtoadepthof10mm.MAINOUTCOMEMEASURES:Following24hoursofreperfusion,functionalneurologicaldeficitswerescored.BraintissuesectionsfromtenratsofeachgroupwereusedtomeasurecerebralinfarctvolumebyTTCstaining.HippocampaltissuesectionsofanadditionaltenratsfromeachgroupwereusedtodetectiNOSlevelsusingthestreptavidin-peroxidaseimmunohistochemis

简介：Interactionsbetweenecologicalcommunitiesofherbivoresandmicrobesarecommonlymediatedbyasharedplant.Atripartiteinteractionbetweenapathogenicfungus-hostplant-herbivorousinsectisanexampleofsuchmutualinfluences.Insuchasystemafungalpathogencommonlyhasanegativeinfluenceonthemorphologyandbiochemistryofthehostplant,withconsequencesforinsectherbivoreperformance.HerewestudiedwhetherthebiotrophicfbngusPodosphaeraferruginea,attackingthegreatburnetSanguisorbaofficinalis,affectscaterpillarperformanceoftheendangeredscarcelargebluebutterflyPhengaristeleius.Ourresultsshowedthatthepathogenicftmgusaffectedthenumberandsizeofinflorescencesproducedbyfood-plantsand,moreimportantly,hadindirect,plant-mediatedeffectsontheabundance,bodymassandimmuneresponseofcaterpillars.Specifically,wefoundtherelationshipbetweencaterpillarabundanceandvariabilityininflorescencesizeonaplanttobepositiveamonghealthyfood-plants,andnegativeamonginfectedfood-plants.Caterpillarsthatfedonhealthyfood-plantsweresmallerthanthosethatfedoninfectedfood-plantsinonestudiedseason,whiletherewasnosuchdifferenceintheotherseason.Weobservedtherelationshipbetweencaterpillarimmuneresponseandtheproportionofinfectedgreatburnetswithinahabitatpatchtobepositivewhencaterpillarsfedonhealthyfood-plants,andnegativewhencaterpillarsfedoninfectedfood-plants.OurresultssuggestthatthisbiotrophicfungalinfectionofthegreatburnetmayimposeasignificantindirectinfluenceonP.teleiuscaterpillarperformancewithpotentialconsequencesforthepopulationdynamicsandstructureofthisendangeredbutterfly.

简介：WRKY抄写因素响应关於生命、不能生活的压力有许多规章的角色。在这研究，我们孤立被稻瘟病真菌Magnaporthegrisea和植物生长素导致的米饭WRKY基因(OsWRKY31)。这基因编码211氨基酸的残余的多肽并且属于可能在单音的简易窄床和dicot植物的分叉以后发源的米饭WRKY基因家庭的亚群。OsWRKY31被发现对洋葱外皮房间的原子核局部性短暂地表示OsWRKY31-eGFP熔化蛋白质。与一个Gal4DNA有约束力的领域熔化的OsWRKY31和它的异种的分析显示OsWRKY31在酵母举办transactivation活动。OsWRKY31基因的Overexpression被发现与M对感染提高抵抗。grisea，和展出的转基因的线减少了侧根形成和延伸与相比野类型并且RNAi植物。线与在表示上显示出许多防卫相关的基因的组成的表示例如PBZ1和OsSci2，以及早植物生长素反应基因，例如OsIAA4和OsCrl1基因。而且，植物与对在高集中的外长地供应的IBA，NAA和2,4-D在表示上不太敏感，在OsWRKY31基因的表示上建议那可能改变植物生长素反应或运输。这些结果也建议OsWRKY31可能在米饭是在植物生长素反应和防卫反应的信号转导小径的一个普通部件。

简介：Smoothened(SMO)是表明小径的刺猬的一个重要成员。我们为RNA干扰构造了特定的recombinantlentiviral向量，指向SMO基因(NM_005631)观察它在人的雄激素敏感的前列腺癌症房间线的SMO表示，房间增长和房间周期上的效果，LNCaP，并且在雄激素无关的前列腺癌症房间线，PC3。四个siRNA序列被设计并且插入了到lentiviral向量pGCSIL-GFP构造四recombinant向量。有最高的介入效率的向量是有在293T房间包装向量(pHelper1.0和pHelper2.0)为分别地感染LNCaP和PC3房间线由liposome装配lentivirus粒子的co-transfected。SMOmRNA，肿瘤房间增长和房间周期的表示水平被量的实时聚合酶链反应(qRT-PCR)测量，3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)试金和流动cytometry分别地。顺序结果证明recombinantlentiviral向量成功地被构造。pGCSIL-GFP-723有最高的介入效率，在co-transfection，LNCaP和PC3房间与排队以后的命名Lv-SIL-SMO723被感染。与控制组相比，显著地显示出的结果减少了(P<0.05)LNCaP和PC3，S阶段房间的更低的吝啬的百分比和G2/M的更高吝啬的百分比的SMOmRNA表情分阶段执行房间，以及显然减缓增长(P<0.01)在感染的组的LNCaP。然而，PC3的增长没被改变(P>0.05)。在结论，recombinantlentivirus粒子能压制SMO表示，在LNCaP和PC3房间线调整房间周期并且显著地禁止LNCaP房间然而并非PC3房间的增长。