简介：VegetativecellsofPorphyrayezoensisareisolatedwithseasnailenzymeandculturedonthesolidifiedagarmedium.Theresultsofexperimentsshowthattheisolatedcellscansurvive,divideandregeneratewellonthemediumsolidifiedwithagar.Thefirstdivisiononthesolidmediumstartsafter7days'culture,4dayslaterthantheliquidculture.Thesurvivalrateofisolatedcellsis71.3%onthesolidmedium,lowerthanthe86.2%ofthatinseawater.Thalli,thalloids,conchocelis,spermatangiaandmulticellularmassesaredevelopedonthesolid/mediuminthefirstmonth,slowlybutnormally.Spermatangiasacsdisappearwithin4weeks.Withoutaddingnutrientliquidontothesurfaceofsolidmediumorinjectingseawaterundertheagarlayerinordertokeepmoisture,thethalliandcellgroupsreleasemonosporestoformnewthalliinsteadofenlargingtheirareasafter5weeks'culturing.Somemonosporesregeneratenewthalli.Othermonosporeslosetheirpigmentsandminimizetheirvolumeanddividequicklytoformlightpinkcalli.After16weeks,numerouscallicanbeseenonthesolidmediumandafter24weeks'culturing,almostonlycalliandconchoceliscanbeseen.Ifthecalliareimmersedinseawater,themonosporesarereleasedandmaydevelopintoyoungthallus.

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简介：客观：到从膝系带的文化成纤维细胞房间并且到学习这些房间的生物特征。方法：前面的十字形的系带(ACL)和从新西兰白兔子的中间的并行的系带(MCL)的房间在vitro是有教养的。骨胶原的细胞的生长和表示被分析。而且，一在里面vitro创伤闭合模型被建立，ACL和MCL房间愈合被比较。结果：为所有这些房间的最大的生长与Dulbecco的家被获得修改的鹰与10%胎儿的牛的浆液补充的媒介，而是RPMI1640和火腿是媒介不是合适的维持这些房间的F12。从新西兰白兔子的ACL和MCL房间的形态学在vitro是相似的，但是MCL房间变得比ACL房间快。两种房间类型在我由ACL房间生产了打III的大杂烩类型的文化，而是比率生产了骨胶原的类似的数量比由MCL房间生产了高。创伤闭合试金证明在在损害以后的36个小时，在ACL文化创造的没有房间的地区被ACL房间部分占据；相反，在MCL文化的受伤地区被房间几乎完全盖住。结论：尽管从新西兰白兔子的ACL细胞和MCL细胞在文化在形态学显示出类似的外观，细胞的生长和象在vitro愈合一样的骨胶原的生物化学的合成是显著地不同的。在在vitro的房间的二种类型的内在的性质的这些差别可能在vivo贡献这些系带的微分愈合潜力。

简介：Litopenaeusvannamei,aeuryhalinespecies,canbeculturedatawiderangeofsalinities.Theemergenceoffreshwaterpond-cultureofL.vannameiisanimportantpreludetothecontinueddevelopmentofshrimpcultureinChina.Inthisstudy,wecomparedtherespiratorymetabolismofjuvenileL.vannameiculturedinfreshwaterandsaltwaterbymeasuringtheiroxygenconsumptionrate(OCR),ammonium-typenitrogenexcretionrate(AER)andpyruvatekinase(PK)andlactatedehydrogenase(LDH)activitiesatdifferentmoltingstagesinordertophysiecologicallycharacterizejuvenileL.vannameiunderfreshwaterconditions.TheresultsshowedthatOCRwassignificantlyhigherinsaltwaterthaninfreshwateratallstagesofmoltingcycle.However,variationofOCRamongmoltingstagesinsaltwaterwassimilarwiththatinfreshwater,andthehighestOCRwasobservedatpost-moltingstage.Atallstagesofmoltingcycle,AERwassignificantlyhigherinfreshwaterthaninsaltwater,andthehighestwasobservedatpost-moltingstage.TheactivityofPKwassignificantlyhigherinsaltwaterthaninfreshwater.Conversely,theactivityofLDHwashigherinfreshwaterthaninsaltwateringeneral.SignificantvariationofPKandLDHactivitiesinmoltingcyclewasobservedinsaltwaterandfreshwater.TheresultsindicatedthataerobicmetabolismofjuvenileL.vannameiwasmoreactiveinsaltwaterthaninfreshwater;whileitsproteinmetabolismwasmoreactiveinfreshwaterthaninsaltwater.

简介：AnargonatmosphericpressureplasmajetwasemployedtotreatL929murinefibroblastsculturedinvitro.Experimentalresultsshowedthat,comparedwiththecontrolcells,thetreatmentoffibroblastswith15sofplasmaledtoasignificantincreaseofcellviabilityandcollagensynthesis,whilethetreatmentof25splasmaresultedinaremarkabledecrease.Explorationofrelatedmechanismssuggestedthatcoldplasmacouldup-regulateCyclinD1geneexpressionanddown-regulatep27geneexpressionatalowdose,whileitcoulddown-regulateCyclinD1expressionandup-regulatep27expressionatahigherdose,thusalteringthecellcycleprogression,andthenaffectingcellviabilityandcollagensynthesisoffibroblasts.

简介：瞄准：在首先有教养的人的胎儿的hepatocytes(HFH)调查肝炎B(HBV)的感染和复制。方法：人的胎儿的hepatocytes在没有浆液的中等、HBV积极的浆液是有教养的被增加进媒介学习hepatocytes的危险性到HBV感染。上层清液为ELISA被收集HBsAg和HBeAg的试金，和为HBV-DNA的量的荧光PCR试金日报。白朊和HBcAg，CK8和CK18表情被免疫组织化学在有教养的hepatocytes检测。lactatedehydrogenate(LDH)的内容被测量发现房间膜的正直。结果：一个稳定的hepatocyte文化系统被建立。HBV能感染hepatocytes并且复制，并且HBcAg表示能被免疫组织化学在象hepatocyte一样房间检测。在上层清液的HBV-DNA能从d被检测2到d18并且HBsAg和HBeAg在d3-d上是积极的18在HBV感染以后。在媒介的HBV从d增加了0到d6并且当房间日益增多地正在松开他们的hepatocyte显型，随后减少了。结论：HBV能感染人的胎儿的hepato-cytes并且复制。这个在试管内模型在与人的HBV入口联系进房间和随后的复制的早事件上允许详细研究。

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简介：嗅觉的ensheathing房间(OEC)是有axonal的glial房间的一种唯一的类型支持生长的性质。OEC移植作为axonal损害和demyelinating疾病的有希望的试验性的治疗出现了。然而，OEC的一些基本细胞的性质仍然保持不清楚。在这研究，我们发现不同OEC候补选手人口基于单个孤立的房间的微速摄影的成像展出了不同迁移的性质，可能由于他们的不同细胞骨架组织。而且，OEC潜水艇人口在单个房间的迁居试金显示了不同吸引人的迁移的回答到lysophosphatidic酸(LPA)的一个坡度。最后，我们发现了人口自发地转变了成对方的那个OEC代用品。一起，这些结果示威，第一次到我们的知识，不同OEC潜水艇人口显示不同迁移的性质试管内并且提供新证据当一个单个房间与韧性的功能的显型打字，支持OEC的观点。

简介：ObjectiveTostudygentamicininjurymechanismsusingpostnatalmousecochlearspiralgangcells(SGC).MethodsSGCswereisolatedusingacombinatorialapproachofenzymaticdigestionandmechanicalseparationfromP2～6Kunmingmousecochleae.After4days,culturedSGCswerefixedwith4%paraformaldehydeatroomtemperatureforimmunocytochemicalexaminationusingthemethodsofS-Pandthemonoclonalantibodyagainstmouseneurofilamentprotein(Neurofilament-68/200Kda,NF-L+H).SGCswererandomlydividedintoablankcontrolgroupandthreegentamicintreatmentgroups(mediumgentamicinconcentrationat50mg/L,100mg/Land150mg/Lrespectively),SGCswerecollectedandexaminedunderatransmissionelectronmicroscopeafterbeingculturedfor48h.ResultsSGCprimaryculturewassuccessful.SGCcytoplasmandneuritesweredyedbrownishyellowbythemonoclonalmouseneurofilamentproteinantibody.SGCsshowedclassicalbipolarneuronappearance.Underthetransmissionelectronmicroscope,.gentamicintreatedSGCsshowedmorphologicalfeaturesdifferentcomparedtothoseintheblankcontrolgroup,whichmightindicateapoptosis.ConclusionOurresultsindicatethatgentamicinhasdirecttoxiceffectsoncochlearSGCsinmiceandtheinjurymechanismiscloselyrelatedwithapoptosis.Damagetomitochondriamayplayanimportantroleintheprocess.

简介：Carbon,nitrogen,phosphorus,siliconcompositionofculturedtwodifferentsizedphytoplanktoncommonspeciesofThalassiosirarotulaandSkeletonemacostatumfromtheJiaozhouBayweremeasured.Carbon,nitrogen,phosphorus,siliconcontentsincellwereobvioushigherinT.rotulathaninS.costatum,butthepercentsofnitrogen,phosphorus,siliconcontentsincelldrymassinT.rotulawerelowerthanthoseinS.costatum.Thedrymassconcentrationsofnitrogen,phosphorus,siliconinS.costatumweremuchhigherthanthoseinT.rotula,particularlysilicon,theformerwas6.4timesofthelatter,showingthatS.costatumcouldmoreassimilatetheseelements.Especially,S.costatumhadcompetitivedominanceforassimilatingsilicon,whichisbeneficialtoitsbecomingamajordominantspeciesinrelativeshortsiliconoftheJiaozhouBay.Thereweresomedifferencesinnumericalvalueofnutrientratiosbothlaboratory-culturedphytoplanktonanddifferentsizedsuspendedparticulates(mainlyphytoplankton)intheJiaozhouBay,whichwascausedbythechangesofenvironment.Highcontentsofcarbon,nitrogonandrelativelowphosporus,silicon,highmolarratioofnitrogentophosphorus(farhigherthanRedfieldvalue)andlowratioofsilicontophosphorusandsilicontonitrogen(farlowerthanRedfieldvalues)inthetwodiatomsanddifferentsizedsuspendedparticulateswereconsistentwiththoseintheseawater.RelativeshortsiliconintheseawaterandphytoplanktonshowedthatsiliconwaspossiblyaffecttingphytoplanktongrowthintheJiaozhouBay.

简介：ＴＢｕｒｎＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅ，ＳｏｕｔｈｗｅｓｔｅｒｎＨｏｓｐｉｔａｌ，ＴｈｉｒｄＭｉｌｉｔａｒｙＭｅｄｉｃａｌＣｏｌｅｇｅ，Ｃｈｏｎｇｑｉｎｇ４０００３８，Ｃｈｉｎａ（ＣｈｉＬＸ，ＹａｎｇＺＣ，ＷａｎｇＸａｎｄＬｉＡ）Ｔｈｉｓｓｔ...

简介：Objective:ToexplorereciprocalactionbetweenBMP-2(bonemorphogeneticprotein-2)andBMP-3forbetterunderstandingofthemechanismofBMPduringbonefractureunion.Methods:rhBMP-2wasaddedintotheculturedfibroblastswiththeconcentrationof1200ng/ml.TheexpressionofBMP-3infibroblastswasdetectedbyimmunohistochemistry.EukaryoticexpressionvectorpcDNA3-BMP-3wastransfectedintothefibroblasts.AftertheeffectiveexpressionofBMP-3wasidentified,BMP-2wasalsodetectedbyimmunohistochemistryinBMP-3expressioncells.ThefibroblaststransfectedwithemptyvectorpcDNA3wereusedasthecontrol.Results:ExogenousrhBMP-2couldpromotetheexpressionofBMP-3infibroblasts.BMP-3alsocouldbedetectedinthesecells.Conclusions:BMP-2andBMP-3couldreciprocallyadjusttheexpressioninfibroblasts.

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简介：细菌和植物可将二氧化碳转化为更有用的化合物，但人类仍在努力通过实践实现这一挑战。现在，面对这一方向开发的催化系统，以高的周转率使CO2还原为CO已迈出新的一步。

简介：ShortinterferingRNA(siRNA)iswidelyusedforstudyingpost-transcriptionalgenesilencingandholdsgreatpromiseasatoolforbothidentifyingfunctionofnovelgenesandvalidatingdrugtargets.TwosiRNAfragments(siRNA-aand-b),whichweredesignedagainstdifferentspecificareasofcodingregionofthesametargetgreenfluorescentprotein(GFP)gene,wereusedtosilenceGFPexpressioninculturedgfptransgeniccellsofrice(OryzasativaL.;OS),cotton(GossypiumhirsutumL.;GH),Fraserfir[Abiesfraseri(Pursh)Poir;AF],andVirginiapine(PinusvirginianaMill.;PV).DifferentialgenesilencingwasobservedinthebombardedtransgeniccellsbetweentwosiRNAs,andtheseresultswereconsistentwiththeinactivationofGFPconfirmedbylaserscanningmicroscopy,Northernblot,andsiRNAanalysisintestedtransgeniccellcultures.ThesedatasuggestthatsiRNA-mediatedgeneinactivationcanbethesiRNAspecificindifferentplantspecies.TheseresultsindicatethatsiRNAisahighlyspecifictoolfortargetedgeneknockdownandforestablishingsiRNA-mediatedgenesilencing,whichcouldbeareliableapproachforlarge-scalescreeningofgenefunctionanddrugtargetvalidation.

简介：GinsenosideRg1（Rg1）hasanti-agingandanti-neurodegenerativeeffects.However,themechanismsunderlyingtheseactionsremainunclear.TheaimofthepresentstudywastodeterminewhetherRg1affectshippocampalsurvivalandneuriteoutgrowthinvitroafterexposuretoamyloid-betapeptidefragment25–35(Aβ25–35),andtoexplorewhethertheextracellularsignal-regulatedkinase（ERK）andAktsignalingpathwaysareinvolvedinthesebiologicalprocesses.Weculturedhippocampalneuronsfromnewbornratsfor24hours,thenaddedRg1tothemediumforanother24hours,withorwithoutpharmacologicalinhibitorsofthemitogen-activatedproteinkinase（MAPK）familyorAktsignalingpathwaysforafurther24hours.Wethenimmunostainedtheneuronsforgrowthassociatedprotein-43,andmeasuredneuritelength.Inaseparateexperiment,weexposedculturedhippocampalneuronstoAβ25–35for30minutes,beforeaddingRg1for48hours,withorwithoutAktorMAPKinhibitors,andassessedneuronalsurvivalusingHoechst33258staining,andphosphorylationofERK1/2andAktbywesternblotanalysis.Rg1inducedneuriteoutgrowth,andthiseffectwasblockedbyAPI-2（Aktinhibitor）andPD98059（MAPK/ERKkinaseinhibitor）,butnotbySP600125orSB203580（inhibitorsofc-JunN-terminalkinaseandp38MAPK,respectively）.Consistentwiththiseffect,Rg1upregulatedthephosphorylationofAktandERK1/2;theseeffectswerereversedbyAPI-2andPD98059,respectively.Inaddition,Rg1significantlyreversedAβ25–35-inducedapoptosis;thiseffectwasblockedbyAPI-2andPD98059,butnotbySP600125orSB203580.Finally,Rg1significantlyreversedtheAβ25–35-induceddecreaseinAktandERK1/2phosphorylation,butAPI-2preventedthisreversal.OurresultsindicatethatRg1enhancesneuriteoutgrowthandprotectsagainstAβ25–35-induceddamage,andthatitsmechanismmayinvolvetheactivationofAktandERK1/2signaling.更多还原

简介：Inthispaper,theauthorspointoutanddemonstratethedifferenceoftheconceptsconcerningco-satisfiableandco-validbetweenonefirst-orderlanguageandtwofirst-orderlanguages,andputforwardtheconceptsaboutuniformco-identicaltruthanduniformco-satisfiability.Thussometheoremsinthebook“ACourseinMathematicalLogic”,writtenbyJ.L.BellandM.Machover,arecorrected.

简介：介绍了Ｐｕ－Ｉ型吸附剂的开发和性能，对它在少量ＣＯ、ＣＯ２脱除和ＣＯ制取工艺的应用也进行了分析。

简介：Thisstudyperformsthequantitativeanalysisandcomparisontoacousticsignalcharact,eristicsofLargcyellowcroaker(Pseudosciaenacrocea)attwodifferentages.Thesoundswercrecordedfromthefishesinanet-cage.Twoexponentialoscillationfunctionsarebuilttofittheacousticsignalofthefishes.Thesignalcharacteristicoftheoscillationfrequencyandattcnuationcoefficientwasdescribedquantitatively.Simulationcurvesofthefunctioncouldfitwellacousticsignals.Boththeaverageoscillationfrequencyandattenuationcoefficientofthefittedsignalsfromthe13-15-month-oldfishesarclowerthanthosefromthe7-8-month-oldfishes.Theresultssuggestthattheoscillationfrequencyandattenuationcoefficientoftheacollsi.icsignalfunctionmayberelevanttothephysicalproccssofsoundproductionandagecharacteristicsofLargeyellowcroaker.Thisstudymaybevaluablefortheacousticapplicationtotheartificialcultureofthespecies.